Microbial contributions to the host metabolome

项目摘要 该建议书包含一个详细的五年培训计划， 新兴科学家专注于了解微生物-宿主相互作用及其对人类疾病的影响。 申请人提出了一个专门构建的研究计划，以实现向 独立的学术研究生涯。通过概述的职业和科学计划，申请人的目标是 利用他之前在技术质谱和微生物学方面的培训， 通量代谢组学、微生物组测序、大规模数据生物信息学和人类糖尿病 生物学在一个结构化和指导的科学环境。莫希特博士和他的同事 Jain和加州大学圣地亚哥分校的Rob Knight博士已经建立，以促进申请人的科学和职业生涯 发展Jain博士和Knight博士的实验室将为申请人提供理想的环境， 国家的最先进的基础设施，分析技能，无数的资源和丰富的科学基础需要获得 必要的培训。此外，两位导师都有良好的指导记录，包括 年轻的研究人员过渡到教师职位和独立实验室。此外，专家 学术咨询委员会的杰出科学家从加州大学圣地亚哥分校和外部科学 社区将在一系列技术和科学领域增加互补的科学专业知识，同时 专注于申请人的职业发展。申请人的中心目标是（1）系统地绘制 存在于人体循环中的数百至数千种肠道微生物衍生的代谢物，以及（2） 确定肠道微生物衍生代谢物在人类糖尿病发展中的作用。对这些 为了实现这些目标，申请人提出利用现有技术的质谱法、微生物组测序和数据 信息学在大规模人口为基础的研究，包括成千上万的人的背景下。 总体而言，从拟议的实验中获得的经验，结构化的职业发展计划， 丰富的科学环境，以及无与伦比的导师和咨询团队将成为 申请人的独立学术生涯。

Project Summary This proposal contains a detailed five-year training program for the mentored career development of an emerging scientist focused on understanding microbe-host interactions and its influence on human disease. The applicant proposes a research program specifically constructed to enable transition towards an independent academic research career. Through the career and scientific plans outlined, the Applicant aims to leverage his prior training in technical mass spectrometry and microbiology with newly acquired skills in high throughput metabolomics, microbiome sequencing, large scale data bioinformatics and human diabetes biology within a structured and mentored scientific environment. A unique joint mentorship between Dr. Mohit Jain and Dr. Rob Knight at UC San Diego has been established to foster the Applicant's scientific and career development. Dr. Jain’s and Dr. Knight’s laboratories will provide an ideal environment for the Applicant, with state-of-the-art infrastructure, analytical skills, innumerable resources and rich scientific base required to obtain the necessary training. Additionally, both mentors have a proven track record of mentorship, including with young investigators transitioning to faculty positions and independent laboratories. Furthermore an expert academic advisory committee of distinguished scientists from the UC San Diego and external scientific community will add complementary scientific expertise in a spectrum of technical and scientific areas, while focused on the career development of the applicant. The applicant's central goal is (1) to systematically map the hundreds to thousands of gut microbial derived metabolites present in human circulation and (2) to determine the role of gut microbial derived metabolites in the development of human diabetes. Towards these goals, the Applicant propose to utilize state of the art mass spectrometry, microbiome sequencing and data informatics within the context of large scale population based studies encompassing thousands of individuals. Collectively, the experience gained from the proposed experiments, structured career development plan, enriched scientific environment, and unmatched mentor and advisory team will serve as the foundation for the applicant’s independent academic career.