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Targeted Exosome-Associated AAV-Mediated Gene Therapy to Eliminate Metastatic Neuroendocrine Cancers

靶向外泌体相关 AAV 介导的基因治疗可消除转移性神经内分泌癌

基本信息

批准号：
9907789
负责人：
Joseph N Garner
金额：
$ 24.31万
依托单位：
TECHNOLOGY COMMERCIALIZATION PARTNERS, LLC
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-07-01 至 2022-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9907789
关键词：
Affinity Animal Model Animals Apoptosis Binding Biodistribution Biological Markers Biological Response Modifier Therapy Bioluminescence Biomanufacturing Bioreactors Bypass Cancer Burden Cancer Patient Cancer cell line Carcinoid Tumor Cell Death Cells Cessation of life Clinic Clinical Trials Cloning Coupled Cytosol Data Dependovirus Diagnosis Diarrhea Drug Kinetics Electron Microscope Endocrine system Energy Metabolism Engineering Enzymes Excision Flow Cytometry Flushing Future Gene Delivery Gene Proteins Genes Goals Heart failure Hormone secretion Hormones IgG1 Impairment In Vitro Inner mitochondrial membrane Islet Cell Tumor Islets of Langerhans Legal patent Light Liver Luciferases Lung Malignant - descriptor Malignant Carcinoid Syndrome Malignant Neoplasms Maximum Tolerated Dose Mediating Membrane Potentials Metastatic Neoplasm to the Liver Mitochondria Modeling Monoclonal Antibodies Morphology Mus Names Neoplasm Metastasis Neoplasms Nervous system structure Neuroendocrine Tumors Neurosecretory Systems Normal Cell Octreotide Operative Surgical Procedures Pancreas Patients Peptide Receptor Pharmaceutical Preparations Play Property Proteins Protons Quality of life Radiation therapy Resistance Rhodopsin Role SDZ RAD SSTR2 gene Safety Scheme Signal Transduction Source Surface Survival Rate System Techniques Technology Therapeutic Therapeutic Effect Time Translations Treatment Efficacy Western Blotting Xenograft procedure anti-cancer base cancer cell cancer therapy chemotherapy clinical efficacy confocal imaging exosome expression vector gene therapy imaging system improved in vivo in vivo imaging system innovation light gated luciferin medullary thyroid carcinoma mitochondrial membrane mouse model neuroendocrine cancer novel optogenetics pre-clinical promoter protein expression response side effect somatostatin receptor 2 subcutaneous targeted treatment therapeutic gene transmission process tumor tumor growth

项目摘要

ABSTRACT Neuroendocrine (NE) malignancies are hormone secreting neoplasms that arise from endocrine and nervous system. Multiple NE tumors (NETs) have been diagnosed, such as pancreatic neuroendocrine cancers, medullary thyroid cancers, and pulmonary neuroendocrine carcinoids. Most NE cancer patients are metastatic at the time of initial diagnosis which makes the complete resections via surgery impossible. The current chemotherapies, including Octreotide, Sunitinib, Everolimus and peptide receptor, have marginal curative benefits and severe side effects. Thus, an effective targeted therapy is critical for patients with metastatic NE cancers. We have recently developed a novel technique, named “mitochondrial chemo-optogenetics”, by expressing a heterologous light-gated channelrhodopsin protein in the IMM of cancer cells, and depolarizing IMM potentials and inducing cell death by using luciferase-luciferin bioluminescence as the endogenous light source. Our preliminary data showed that this new mitochondrial gene therapy caused substantial NE cancer cell death in vitro and stopped NE tumor growth and even reduced tumor size in a subcutaneous NE cancer xenograft mouse model. Additionally, we have built an innovative NE cancer-targeted gene delivery platform by tagging our new anti-somatostatin receptor 2 (SSTR2) monoclonal antibody (mAb) to the surface of exosome. However, a targeted gene therapy, such as mAb-Exo-AAV carrying our mitochondrial chemo-optogenetics therapeutic gene, is urgently needed to achieve substrate-induced mitochondrial depolarization and selective elimination of cancer cells in vivo. Moreover, the therapeutic efficacy of the gene therapy in metastatic a model is essential because most diagnosed NE cancer patients are metastatic. The specific objective of this application is to develop, produce and evaluate an innovative NE cancer-targeted mitochondrial gene therapy to selectively destroy and eliminate NETs in vivo. The following two specific aims over a 12-month period are propose. Aim 1: To develop, produce and characterize the NE-cancer targeted mitochondrial gene therapy. A high-quality anti-SSTR2 mAb-Exo-AAV will be constructed by cloning a cancer promoter (cfos) and the fused blue light- producing luciferase and light-gated rhodopsin gene, i.e. cfos-NLuc-2A-ABCB-CoChR (~3.3 kb), into the engineered pAAV-MCS promoterless expression vector, and produced using our stirred-tank bioreactor-based exosome-AAV biomanufacturing platform and surface tagging technology. The anti-SSTR2 mAb-Exo-AAV will then be evaluated for its cancer specific targeting and in vitro anti-cancer efficacy. Aim 2: To evaluate the therapeutic values of the mitochondrial gene therapy using preclinical NET metastatic animal model. Most NE cancer patients are initially diagnosed with metastases and have already developed carcinoid syndrome. Therefore we will evaluate the maximal tolerated dose (MTD), pharmacokinetics (PK), anti- NET efficacy, and liver metastases reduction of the developed gene therapy using metastatic model.

摘要