Generation of feeding decisions in the fly brain

果蝇大脑中进食决策的生成

• 批准号: 9908070
• 负责人: Gabriella Rose Sterne
• 金额: $ 6.93万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-05-01 至 2021-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9908070
• 关键词: Afferent Neurons; Anatomy; Animal Model; Animals; Behavior; Behavioral; Biological Models; Brain; Caenorhabditis elegans; Collection; Comparative Study; Complex; Decision Making; Diabetes Mellitus; Disease; Drosophila genus; Environment; Feeding behaviors; Food; Generations; Genetic; Genetic Epistasis; Hunger; Individual; Ingestion; Insecta; Interneurons; Knowledge; Label; Mammals; Modality; Molecular Genetics; Motor; Motor Neurons; Movement; Mus; Nervous system structure; Neurons; Obesity; Olfactory Pathways; Organ; Organism; Output; Palate; Positioning Attribute; Process; Recording of previous events; Sensory; Societies; Sodium Chloride; Synapses; Taste Perception; Translating; Vertebral column; Water; Work; cell type; design; detector; experimental study; feeding; fly; food quality; human disease; in vivo; in vivo calcium imaging; insect disease; insight; neural circuit; neural network; neuron component; novel; programs; relating to nervous system; sugar; transmission process

Appropriate feeding decisions are essential to individual and species survival. In Drosophila, like mammals, neural circuits integrate information about food quality and internal nutritive state to generate feeding decisions. However, the neural circuitry that arrives at and carries out feeding decisions is unknown. Here, we propose to produce the first circuit-level view of Drosophila feeding decision-making circuitry. We will define feeding circuits starting at a previously identified subesophageal zone (SEZ) interneuron, Feeding neuron (Fdg), which generates a complex feeding sequence when activated. Furthermore, Fdg only responds to presentation of palatable food in starved flies, suggesting that Fdg receives information about both food quality and hunger state. Using candidate synaptic partners identified in a preliminary behavioral screen of a novel SEZ split-Gal4 collection, we will define the interneurons that relay information about hunger state and food quality to Fdg and investigate how neurons downstream of Fdg work together to generate a feeding motor sequence. The completion of these experiments will produce the first-ever functional wiring diagram of Drosophila gustatory circuitry and will provide insight into the types of computations neural circuits perform to generate and carry out feeding decisions. Since complex neural networks are thought to arise by extending and combining simple networks arising earlier in evolutionary history, the insights gained by delineating Drosophila feeding circuitry will form the basis for understanding the neural circuits that generate feeding decisions in more complex organisms.

适当的喂养决策对个体和物种的生存至关重要。在 果蝇，像哺乳动物一样，神经回路整合有关食物质量和内部的信息。 营养状态，以产生喂养决策。然而，神经回路到达和 执行喂养决策的情况不明。在这里，我们建议生成第一个电路级视图 果蝇的进食决策回路。我们将定义馈电回路， 先前确定的食管下区（SEZ）中间神经元，摄食神经元（Fdg）， 激活后会产生复杂的进食顺序此外，Fdg仅响应于 在饥饿的苍蝇中呈现可口的食物，这表明Fdg接收到关于 食物质量和饥饿状态。使用在初步研究中确定的候选突触伴侣， 行为屏幕的一个新的经济特区分裂Gal4收集，我们将定义中间神经元，中继 关于饥饿状态和食物质量的信息，并研究神经元如何 下游的Fdg一起工作以产生进给马达序列。完成 这些实验将产生有史以来第一个果蝇味觉功能线路图 电路，并将提供洞察类型的计算神经电路执行， 产生并执行喂养决策。由于复杂的神经网络被认为是 通过扩展和组合进化史上早期出现的简单网络， 通过描绘果蝇的进食回路获得的信息将成为理解 在更复杂的生物体中产生进食决定的神经回路。