Automated Multichannel Bidirectional Fiber Optic Rotary Device for Brain-Behavior Studies

这里提出的项目解决了对允许微创的方法的迫切需求 在小鼠大脑深处以高空间和高时间分辨率进行长期光学记录。 了解脑神经活动和行为之间的因果关系也在研究之列 这是NIAAA的优先事项，也是大脑倡议的主要目标之一。已经取得了很大进展 对行为过程中的神经回路动态进行光学扰动和监测。然而，实际情况是 实施在很大程度上仍然局限于一次大脑中的一个位置，仍然有迫切的需要 用于在大脑深处的多个位置同时进行微创长时间光学记录 空间和时间分辨率。多个地点的同时录音将使神经科学家获得 通过构建关于生化信号的时间和同步性的全球视图来获得新的见解 神经元、投射和不同的大脑区域。他们阐明了正常背后的神经机制 与酒精成瘾、药物成瘾、帕金森氏症等相关的行为和行为 自由活动啮齿动物的神经肌肉和神经精神疾病。 拟议的项目旨在设计一种自动化的多通道双向ForJ，将允许 通过旋转将光信号同时传输到大脑的多个位置或从大脑的多个位置同时传输 界面，对自由移动的鼠标的自然行为的机械影响最小。第一 目的是建立一个双通道双向FORJ原型装置。简而言之，设计原则是 使用角度编码器动态测量鼠标的移动并自动释放 通过接合无刷直流电机将缠绕的光纤解绕n，从而使光缆发生扭转 360度转弯次数。为了优化其效率，仅自动激活展开过程 当角度编码器达到实验确定的阈值时。第二个目标是执行 验证和定量表征总体光吞吐量、传输变化的测试 旋转、启动扭矩以及forJ与数据采集的同步。最后，在 两通道为J，四通道为J，将通过扩展通道容量来构建。该项目 在本申请中提出了一种独特的方法，将光学设计和旋转传感相结合 为了降低光学设计的复杂性，减少机械运动部件的数量，以便 提高信噪比。多通道双向ForJ潜在地提供酒精成瘾 研究社区扩大了调查酒精对学习和决策的影响的能力。 更广泛地说，它为神经科学界提供了研究因果关系的可能性 神经回路的动态生化活动与跨越空间和时间尺度的行为之间的关系。

The project proposed herein addresses an urgent need for approaches that allow minimally invasive long term optical recording deep in a mouse brain with high spatial and high temporal resolution. Understanding the causal relationship between brain neural activity and behavior is among the research priorities of NIAAA and one of the main objectives of the BRAIN initiative. A lot of progress has been made to optically perturb and monitor neural circuit dynamics during behavior. However, the practical implementation is still largely limited to one location in the brain at a time, and there is still an urgent need for simultaneous minimally invasive long time optical recording deep in the brain at multiple sites with spatial and temporal resolution. Simultaneous recordings at multiple sites will allow neuroscientists to gain new insights by constructing a global view on the timing and synchrony of biochemical signals among neurons, projections, and different brain regions. They shed light on neural mechanisms underlying normal behavior and behavior associated with alcohol addiction, drug addiction, Parkinson’s disease, and other neuromuscular and neuropsychiatric diseases in freely moving rodents. The proposed project aims to design an automated multichannel bidirectional FORJ that will allow the simultaneous transfer of optical signals to and from multiple sites of the brain through a rotating interface and with minimal mechanical impact on the natural behavior in a freely moving mouse. The first aim is to build a two-channel bidirectional FORJ prototype device. The design principle, in a nutshell, is to dynamically measure, using an angle encoder, the movement of a mouse and automatically release the torsion in the optical cable by engaging a brushless DC motor to unwind the twisted optical fiber by n number of 360° turns. To optimize its efficiency, the unwinding process is activated automatically only when the angle encoder reaches an experimentally determined threshold. The second aim is to perform tests to validate and quantitatively characterize overall light throughput, transmission variation over rotation, starting torque, and synchronization of the FORJ with data acquisition. Finally, on the basis of the two-channel FORJ, a four-channel FORJ will be built by extending the channel capacity. The project proposed in this application takes a unique approach by combining optical design and rotational sensing to reduce the optical design complexity and reduce the number of mechanical moving parts in order to improve signal-to-noise ratio. The multichannel bidirectional FORJ potentially offers the alcohol addiction research community expanded capability to investigate ethanol effects on learning and decision making. More broadly, it offers the neuroscience community the possibilities to investigate the causal relationship between dynamic biochemical activity of neuronal circuits and behavior across spatial and temporal scales.