ShEEP request for next generation sequencing system

ShEEP 请求下一代测序系统

• 批准号: 9906671
• 负责人: Nikhil C. Munshi
• 金额: --
• 依托单位: VA BOSTON HEALTH CARE SYSTEM
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2020-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9906671
• 关键词: Alcoholism; Alzheimer' s Disease; Cells; Chromium; Clinical; Coagulation Process; Code; Collaborations; Colorectal; Complement; Data; Development; Ensure; Equipment; Funding; Genetic; Genomics; Gold; Health; Human Genome; Institutes; Kidney; Label; Lead; Libraries; Maintenance; Malignant Neoplasms; Massachusetts; Molecular Target; Multiple Myeloma; Neurosciences; Output; Post-Traumatic Stress Disorders; Procedures; Reagent; Research; Research Personnel; Research Project Grants; Running; Scanning; Sheep; Site; Sleep; Spinal Cord; Standard Model; Stem cells; System; Talents; Technology; Time; Training; Veterans; Work; exome sequencing; flexibility; improved; instrument; medical schools; next generation sequencing; operation; programs; research facility; single cell sequencing; single-cell RNA sequencing; transcriptome; transcriptome sequencing; whole genome

The requested sequencing equipment is state-of-the-art sequencing machine, versatile, and will be used by all funded VA investigators at the VABHS. This is Illumina NextSeq-550 Sequencing System with 10X genomics controller to make single-cell-libraries. Our researchers at VABHS investigate diverse fields of research including cancer (myeloma & colorectal) and neuroscience (Alzheimer's, sleep, CTE, TBI & PTSD) as well as other fields of research (kidney, spinal cord, stem cells, coagulation, & alcoholism) that are aimed at aiding veterans. Currently, VA-funded projects have specifically outlined procedures using NGS sequencing and microarray scanning instruments, but they must use non-VA facilities to perform their VA research. We describe that VA investigators do not currently have the requested sequencing equipment at any VABHS research facility is a major need for this equipment. The equipment will also allow for exciting new developments for VA investigators research projects and give new opportunities for collaborations between VA and non-VA investigators. Obtaining this equipment can also strengthen new funding proposals and lead to collaborations including those that will complement genetic wide-association studies such as the Million Veteran Program. Lastly, having this equipment on site will allow us to attract and retain the best possible basic and clinical scientific talent in the world on topics that will serve our veterans. Drs. Zielinski and Prabhala will be responsible for being fully informed of the equipment operation and maintenance and will ensure that users are trained in flow cell cleaning and general- purpose cleaning.

请求的测序设备是最先进的测序机器，并且将由所有人使用 VABHS的VA资助调查人员。这是具有10倍基因组学的Illumina NextSeq-550测序系统 控制器制作单细胞上线。我们在Vabhs的研究人员研究了各种研究领域 癌症（骨髓瘤和结直肠癌）和神经科学（阿尔茨海默氏症，睡眠，CTE，TBI和PTSD）以及其他领域 旨在帮助退伍军人的研究（肾脏，脊髓，干细胞，凝血和酒精中毒）的研究。 目前，由VA资助的项目专门概述了使用NGS测序和微阵列的程序 扫描工具，但他们必须使用非VA设施来进行VA研究。我们描述了VA 研究人员目前尚未在任何VABHS研究设施中都有要求的测序设备 该设备的主要需求。该设备还将为VA调查人员提供令人兴奋的新发展 研究项目并为VA与非VA调查人员之间的合作提供了新的机会。获得 该设备还可以加强新的资金建议，并导致合作，包括 补充遗传广泛关联研究，例如百万退伍军人计划。最后，拥有此设备 现场将使我们能够吸引和保留世界上最好的基本和临床科学人才 这将为我们的退伍军人服务。博士。 Zielinski和Prabhala将负责完全了解 设备操作和维护，并将确保用户接受流动池清洁和一般培训 - 目的清洁。