ShEEP request for next generation sequencing system

ShEEP 请求下一代测序系统

• 批准号: 9906671
• 负责人: Nikhil C. Munshi
• 金额: --
• 依托单位: VA BOSTON HEALTH CARE SYSTEM
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2020-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9906671
• 关键词: Alcoholism; Alzheimer' s Disease; Cells; Chromium; Clinical; Coagulation Process; Code; Collaborations; Colorectal; Complement; Data; Development; Ensure; Equipment; Funding; Genetic; Genomics; Gold; Health; Human Genome; Institutes; Kidney; Label; Lead; Libraries; Maintenance; Malignant Neoplasms; Massachusetts; Molecular Target; Multiple Myeloma; Neurosciences; Output; Post-Traumatic Stress Disorders; Procedures; Reagent; Research; Research Personnel; Research Project Grants; Running; Scanning; Sheep; Site; Sleep; Spinal Cord; Standard Model; Stem cells; System; Talents; Technology; Time; Training; Veterans; Work; exome sequencing; flexibility; improved; instrument; medical schools; next generation sequencing; operation; programs; research facility; single cell sequencing; single-cell RNA sequencing; transcriptome; transcriptome sequencing; whole genome

The requested sequencing equipment is state-of-the-art sequencing machine, versatile, and will be used by all funded VA investigators at the VABHS. This is Illumina NextSeq-550 Sequencing System with 10X genomics controller to make single-cell-libraries. Our researchers at VABHS investigate diverse fields of research including cancer (myeloma & colorectal) and neuroscience (Alzheimer's, sleep, CTE, TBI & PTSD) as well as other fields of research (kidney, spinal cord, stem cells, coagulation, & alcoholism) that are aimed at aiding veterans. Currently, VA-funded projects have specifically outlined procedures using NGS sequencing and microarray scanning instruments, but they must use non-VA facilities to perform their VA research. We describe that VA investigators do not currently have the requested sequencing equipment at any VABHS research facility is a major need for this equipment. The equipment will also allow for exciting new developments for VA investigators research projects and give new opportunities for collaborations between VA and non-VA investigators. Obtaining this equipment can also strengthen new funding proposals and lead to collaborations including those that will complement genetic wide-association studies such as the Million Veteran Program. Lastly, having this equipment on site will allow us to attract and retain the best possible basic and clinical scientific talent in the world on topics that will serve our veterans. Drs. Zielinski and Prabhala will be responsible for being fully informed of the equipment operation and maintenance and will ensure that users are trained in flow cell cleaning and general- purpose cleaning.

所要求的测序设备是最先进的测序机，多功能，将被所有人使用 资助了退伍军人事务部的调查员这是Illumina NextSeq-550测序系统，具有10 X基因组学 控制器来制作单细胞库。我们的研究人员在VABHS调查研究的不同领域，包括 癌症（骨髓瘤和结直肠）和神经科学（阿尔茨海默氏症，睡眠，CTE，TBI和PTSD）以及其他领域 研究（肾脏，脊髓，干细胞，凝血和酗酒），旨在帮助退伍军人。 目前，VA资助的项目已经具体概述了使用NGS测序和微阵列的程序 扫描仪器，但他们必须使用非VA设施进行VA研究。我们描述了VA 研究人员目前在任何VABHS研究机构都没有要求的测序设备， 这是设备的主要需求。该设备还将为退伍军人事务部调查人员带来令人兴奋的新发展 研究项目，并为VA和非VA调查人员之间的合作提供新的机会。获得 这种设备还可以加强新的资金建议，并导致合作，包括那些将 补充遗传广泛关联研究，如百万退伍军人计划。最后，拥有这些设备 现场将使我们能够吸引和留住世界上最好的基础和临床科学人才的主题 为我们的退伍军人服务Zielinski博士和Prabhala博士将负责充分了解 设备操作和维护，并确保用户接受流动池清洁和一般培训- 目的清洗。