ShEEP Request for BD FACSAria Fusion Cell Sorting Flow Cytometer

本提案描述了购买BD FACSAria Fusion细胞分选流程的资金申请 细胞计数器，4个激光器。在本建议书中，我们描述了我们获得设备的需求， 技术规格，新合作的潜力和新的研究途径， 将帮助退伍军人，并计划管理的设备。流式细胞仪 广泛应用于各种研究领域，该设备的使用特别是 概述并用于VA波士顿医疗保健系统（VABHS）的VA资助项目。细胞 分选流式细胞仪在任何VABHS研究设施中都不存在。然而，由于VABHS 调查人员目前必须使用附属机构，这会增加成本、时间和样本 可行性和程序问题限制了我们目前的能力。此外，最近在 技术被应用于VABHS的不同研究领域， 流式细胞术可用于确定细胞特异性靶。流式细胞仪，4 激光将允许细胞分选和分析多达11个生物标志物同时在细胞中， 一个极其多样化的细胞群。所要求的设备有几个 增强其功能和操作员安全的能力。具有细胞分选流程 细胞计数器的能力将显著提高我们理解病理生理学的能力， 许多影响退伍军人的疾病，包括癌症，睡眠相关疾病， 精神分裂症VABHS拥有细胞分选流式细胞术能力， 新的合作，吸引和留住一流的研究人员。此外，该系统还 包含在一个保护罩，这将加强对退伍军人事务部调查员的保护。

This proposal describes a request for funds to purchase a BD FACSAria Fusion cell sorting flow cytometer with 4 lasers. In this proposal, we describe our needs for obtaining the equipment, its technical specifications, the potential for new collaborations and new avenues of research that will aid veterans, and a plan for the administration of the equipment. Cell sorting flow cytometers are widely used in a variety of research fields and the use of this equipment is specifically outlined and used in VA funded projects at the VA Boston Healthcare System (VABHS). A cell sorting flow cytometer does not exist in any VABHS research facility. However, since VABHS investigators currently must use affiliated institutions, there are added costs, time, and sample viability and procedures issues that limit our current abilities. Further, recent advances in technology are being applied in the diverse fields of research at the VABHS where cell sorting flow cytometry can be used to determine cell specific targets. The FACS equipment with 4 lasers will allow for cell sorting and analysis of up to 11 biomarkers simultaneously in cell population for an extremely diverse set of cells. The requested equipment has several capabilities that enhance its function and the safety of the operators. Having cell sorting flow cytometer capabilities will markedly enhance our abilities to understanding the pathophysiology of numerous disorders that affect veterans including cancer, sleep-related disorders, and schizophrenia. Possessing cell sorting flow cytometry capabilities at the VABHS will allow for new collaboration and attract and retain top-rate researchers. Also, this system is also contained in a protective hood that will enhance the protection of VA investigators.