ShEEP Request for BD FACSAria Fusion Cell Sorting Flow Cytometer

本提案描述了购买BD FACSAria融合细胞分选流程的资金请求 带有4个激光的细胞仪。在这份建议书中，我们描述了我们获得设备的需求，其 技术规范、新合作的潜力和新的研究途径 将帮助退伍军人，并制定设备管理计划。细胞分选流式细胞仪 被广泛应用于各种研究领域，而这种设备的使用特别是 在退伍军人管理局波士顿医疗系统(VABHS)的退伍军人管理局资助的项目中概述和使用。一间牢房 VABHS的任何研究机构都不存在分选流式细胞仪。然而，由于VABHS 调查人员目前必须使用附属机构，还有增加的成本、时间和样本 生存能力和程序问题限制了我们目前的能力。此外，最近在以下方面的进展 技术被应用于VABHS的不同研究领域，其中细胞分选 流式细胞术可以用来确定细胞的特异性靶点。FACS设备有4个 激光将允许在细胞内同时对多达11个生物标志物进行细胞分选和分析 一组极其多样化的细胞的种群。所请求的设备有几个 增强其功能和操作员安全的能力。具有细胞分选流程 细胞测定仪的功能将显著提高我们理解病理生理的能力 影响退伍军人的众多疾病，包括癌症，睡眠相关障碍，以及 精神分裂症。在VABHS拥有细胞分选流式细胞仪能力将允许 新的协作，吸引和留住一流的研究人员。另外，这个系统也是 包含在保护罩中，将加强对退伍军人管理局调查人员的保护。

This proposal describes a request for funds to purchase a BD FACSAria Fusion cell sorting flow cytometer with 4 lasers. In this proposal, we describe our needs for obtaining the equipment, its technical specifications, the potential for new collaborations and new avenues of research that will aid veterans, and a plan for the administration of the equipment. Cell sorting flow cytometers are widely used in a variety of research fields and the use of this equipment is specifically outlined and used in VA funded projects at the VA Boston Healthcare System (VABHS). A cell sorting flow cytometer does not exist in any VABHS research facility. However, since VABHS investigators currently must use affiliated institutions, there are added costs, time, and sample viability and procedures issues that limit our current abilities. Further, recent advances in technology are being applied in the diverse fields of research at the VABHS where cell sorting flow cytometry can be used to determine cell specific targets. The FACS equipment with 4 lasers will allow for cell sorting and analysis of up to 11 biomarkers simultaneously in cell population for an extremely diverse set of cells. The requested equipment has several capabilities that enhance its function and the safety of the operators. Having cell sorting flow cytometer capabilities will markedly enhance our abilities to understanding the pathophysiology of numerous disorders that affect veterans including cancer, sleep-related disorders, and schizophrenia. Possessing cell sorting flow cytometry capabilities at the VABHS will allow for new collaboration and attract and retain top-rate researchers. Also, this system is also contained in a protective hood that will enhance the protection of VA investigators.