ShEEP Request for Acquisition a 10x Genomics Single Cell RNA Sequencer

ShEEP 请求购买 10x Genomics 单细胞 RNA 测序仪

• 批准号: 9910032
• 负责人: JOSEPH R PISEGNA
• 金额: --
• 依托单位: VA GREATER LOS ANGELES HEALTHCARE SYSTEM
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2020-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9910032
• 关键词: Alzheimer' s Disease; Appetite Regulation; B-Lymphocytes; Biological Process; Biology; Cell Nucleus; Cell Surface Proteins; Cells; Chromatin; Chromium; Colon Carcinoma; Communicable Diseases; Complex; Computer software; Copy Number Polymorphism; Critical Pathways; DNA; DNA copy number; Data; Data Analyses; Development; Developmental Biology; Diabetes Mellitus; Disease; Encapsulated; Epithelial Cell Proliferation; Equipment; Excision; Fatty Liver; Funding; Gel; Gene Expression; Gene Expression Profiling; Genes; Genetic Transcription; Genomics; Goals; Guide RNA; Health; Healthcare; Healthcare Systems; Hematopoiesis; Heterogeneity; Human; Hypothyroidism; Immune; Immunology; Individual; Inflammation; Institution; Intestines; Length; Leptospirosis; Libraries; Los Angeles; Malignant Neoplasms; Malignant neoplasm of prostate; Manufacturer Name; Measurement; Medical center; Metabolic syndrome; Modeling; Motor; Mus; Myocardium; Natural regeneration; Nerve Regeneration; Neurophysiology - biologic function; Neurosciences; Obesity; Organ; Parkinson Disease; Pathway interactions; Permeability; Phenotype; Population; Population Heterogeneity; Process; Program Evaluation; RNA Sequences; Reaction; Regulation; Regulator Genes; Regulatory Pathway; Research; Research Personnel; Research Project Grants; Resolution; Resources; Sampling; Sheep; Signal Pathway; Signal Transduction; Spinal cord injury; Stress; T-Lymphocyte; Technology; Tissues; Transcript; Transitional Cell; Ventricular; Veterans; Visceral pain; XCL1 gene; adaptive immune response; base; career development; cell type; cost; digital; epigenome; experimental study; genome analysis; immune function; improved; insight; microbial; post stroke; response; sequencing platform; single cell analysis; single cell sequencing; single cell technology; single-cell RNA sequencing; stressor; transcriptome; transcriptome sequencing; transcriptomics

Project Summary/Abstract Single cell transcriptomic technology is a new and essential technology that allows for the direct measurement of gene expression at the single cell level to quantify cell population intracellular heterogeneity and characterize cell phenotypes, cell signaling pathways and functions, and dynamic cellular transitions cell by cell. This provides an analysis at the single cell level thus providing insights into biology that have been previously inaccessible and also at a greatly reduced cost. The 10x Single Cell RNAseq (scRNA-seq) Chromium Single Cell Gene Expression Controller provides an unparalleled approach for uncovering cell-to-cell gene expression variability and identifying rare cell types in heterogeneous samples derived from tissue or cells. This single cell sequencing platform will allow VA researchers to extend beyond traditional global marker gene analysis to characterize cell types or cell functional states and the concomitant dynamic gene changes in regulatory pathways. scRNA-seq is now widely employed in studies seeking to resolve previously under-recognized cellular heterogeneity, define key processes in cell development and differentiation, unravel critical pathways of hematopoiesis, and understand the gene regulatory networks that predict immune function. A static snapshot of single-cell transcriptomes can provide a powerful window onto the various stages of differentiation and activation states, which are rarely synchronized between cells. Importantly, complex biological processes in developmental biology, cancer, neuroscience, immunology, and infectious disease usually involve multiple individual cells that have different fates, states, and functions. These can be now characterized, captured and resolved at the cellular level with a single scRNA-seq experiment. This single cell RNA sequencer with the Chromium Single Cell Gene Expression Solution will provide an unparalleled resource for all of the VA research investigators at our VA Medical Center Greater Los Angeles Healthcare System. The 10x Single Cell RNAseq will support the following VA funded Merit Review and Career Development research projects that have the ultimate goal to improve and help Veterans healthcare at our institution: 1. Treatments for neural regeneration, such as restoring neural function after stroke or spinal cord injury, 2. Hypothyroidism, 3. Microbial regulation of appetite, obesity, metabolic syndrome, diabetes and fatty liver, 4. Prostate cancer, 5. Alzheimer disease, 6. Leptospirosis, 7. Regenerating myocardium after ventricular resection, 8. Modulation of colonic response to stress and inflammation, 9. Intestinal epithelial cell proliferation and permeability, 10. Regulation of gut motor function, and visceral pain altered by various stressors, 11. Gut bacterial metabolites, 12. Parkinson’s disease, and 13. Colon cancer.

项目总结/文摘