ShEEP Request for Acquisition a 10x Genomics Single Cell RNA Sequencer

ShEEP 请求购买 10x Genomics 单细胞 RNA 测序仪

• 批准号: 9910032
• 负责人: JOSEPH R PISEGNA
• 金额: --
• 依托单位: VA GREATER LOS ANGELES HEALTHCARE SYSTEM
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2020-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9910032
• 关键词: Alzheimer' s Disease; Appetite Regulation; B-Lymphocytes; Biological Process; Biology; Cell Nucleus; Cell Surface Proteins; Cells; Chromatin; Chromium; Colon Carcinoma; Communicable Diseases; Complex; Computer software; Copy Number Polymorphism; Critical Pathways; DNA; DNA copy number; Data; Data Analyses; Development; Developmental Biology; Diabetes Mellitus; Disease; Encapsulated; Epithelial Cell Proliferation; Equipment; Excision; Fatty Liver; Funding; Gel; Gene Expression; Gene Expression Profiling; Genes; Genetic Transcription; Genomics; Goals; Guide RNA; Health; Healthcare; Healthcare Systems; Hematopoiesis; Heterogeneity; Human; Hypothyroidism; Immune; Immunology; Individual; Inflammation; Institution; Intestines; Length; Leptospirosis; Libraries; Los Angeles; Malignant Neoplasms; Malignant neoplasm of prostate; Manufacturer Name; Measurement; Medical center; Metabolic syndrome; Modeling; Motor; Mus; Myocardium; Natural regeneration; Nerve Regeneration; Neurophysiology - biologic function; Neurosciences; Obesity; Organ; Parkinson Disease; Pathway interactions; Permeability; Phenotype; Population; Population Heterogeneity; Process; Program Evaluation; RNA Sequences; Reaction; Regulation; Regulator Genes; Regulatory Pathway; Research; Research Personnel; Research Project Grants; Resolution; Resources; Sampling; Sheep; Signal Pathway; Signal Transduction; Spinal cord injury; Stress; T-Lymphocyte; Technology; Tissues; Transcript; Transitional Cell; Ventricular; Veterans; Visceral pain; XCL1 gene; adaptive immune response; base; career development; cell type; cost; digital; epigenome; experimental study; genome analysis; immune function; improved; insight; microbial; post stroke; response; sequencing platform; single cell analysis; single cell sequencing; single cell technology; single-cell RNA sequencing; stressor; transcriptome; transcriptome sequencing; transcriptomics

Project Summary/Abstract Single cell transcriptomic technology is a new and essential technology that allows for the direct measurement of gene expression at the single cell level to quantify cell population intracellular heterogeneity and characterize cell phenotypes, cell signaling pathways and functions, and dynamic cellular transitions cell by cell. This provides an analysis at the single cell level thus providing insights into biology that have been previously inaccessible and also at a greatly reduced cost. The 10x Single Cell RNAseq (scRNA-seq) Chromium Single Cell Gene Expression Controller provides an unparalleled approach for uncovering cell-to-cell gene expression variability and identifying rare cell types in heterogeneous samples derived from tissue or cells. This single cell sequencing platform will allow VA researchers to extend beyond traditional global marker gene analysis to characterize cell types or cell functional states and the concomitant dynamic gene changes in regulatory pathways. scRNA-seq is now widely employed in studies seeking to resolve previously under-recognized cellular heterogeneity, define key processes in cell development and differentiation, unravel critical pathways of hematopoiesis, and understand the gene regulatory networks that predict immune function. A static snapshot of single-cell transcriptomes can provide a powerful window onto the various stages of differentiation and activation states, which are rarely synchronized between cells. Importantly, complex biological processes in developmental biology, cancer, neuroscience, immunology, and infectious disease usually involve multiple individual cells that have different fates, states, and functions. These can be now characterized, captured and resolved at the cellular level with a single scRNA-seq experiment. This single cell RNA sequencer with the Chromium Single Cell Gene Expression Solution will provide an unparalleled resource for all of the VA research investigators at our VA Medical Center Greater Los Angeles Healthcare System. The 10x Single Cell RNAseq will support the following VA funded Merit Review and Career Development research projects that have the ultimate goal to improve and help Veterans healthcare at our institution: 1. Treatments for neural regeneration, such as restoring neural function after stroke or spinal cord injury, 2. Hypothyroidism, 3. Microbial regulation of appetite, obesity, metabolic syndrome, diabetes and fatty liver, 4. Prostate cancer, 5. Alzheimer disease, 6. Leptospirosis, 7. Regenerating myocardium after ventricular resection, 8. Modulation of colonic response to stress and inflammation, 9. Intestinal epithelial cell proliferation and permeability, 10. Regulation of gut motor function, and visceral pain altered by various stressors, 11. Gut bacterial metabolites, 12. Parkinson’s disease, and 13. Colon cancer.

项目摘要/摘要 单细胞转录组技术是一种新的基本技术，可以直接测量 在单细胞水平上的基因表达，以量化细胞群体内异质性并表征细胞 表型，细胞信号通路和功能以及动态细胞通过细胞转变。这提供了 在单细胞水平上的分析，从而提供了对以前无法访问的生物学的见解， 也以大大降低的成本。 10倍单细胞RNASEQ（SCRNA-SEQ）铬单细胞基因表达 控制器提供了一种无与伦比的方法，用于发现细胞对细胞基因表达变异性和 在组织或细胞的异质样品中鉴定罕见的细胞类型。这个单细胞测序 平台将使VA研究人员能够超越传统的全球标记基因分析，以表征细胞 类型或细胞功能状态以及调节途径中伴随的动态基因的变化。 scrna-seq 现在已广泛用于寻求解决以前未经认可的细胞异质性的研究，定义 细胞发育和分化的关键过程，造血的关键途径以及 了解预测免疫功能的基因调节网络。单细胞的静态快照 转录组可以为分化和激活状态的各个阶段提供一个强大的窗口， 很少在细胞之间同步。重要的是，发育中的复杂生物过程 生物学，癌症，神经科学，免疫学和传染病通常涉及多个单个细胞 具有不同的命运，状态和功能。这些现在可以在细胞上进行表征，捕获和解决 与单个SCRNA-SEQ实验保持水平。该单细胞RNA测序仪与铬单细胞基因 表达解决方案将为我们VA的所有VA研究人员提供无与伦比的资源 大洛杉矶医疗中心医疗保健系统。 10倍单小区RNASEQ将支持以下 VA资助了绩效审查和职业发展研究项目，其最终目标是改进和 帮助我们机构的退伍军人医疗保健：1。神经发生的治疗方法，例如恢复神经元 中风或脊髓损伤后的功能，2。甲状腺功能减退症，3。食欲，肥胖，肥胖症的微生物调节 代谢综合征，糖尿病和脂肪肝，4。前列腺癌，5。阿尔茨海默氏病，6。钩端螺旋体病，7。 心室切除后再生心肌，8。对压力和压力的反应调节 炎症，9。肠上皮细胞增殖和渗透率，10。 内脏疼痛因各种压力源而改变，肠道细菌代谢物，12个。帕金森氏病和13个。结肠 癌症。