ShEEP Request for IVIS SPECTRUMCT, 2D and 3D Optical In Vivo Tomography System.

ShEEP 请求 IVIS SPECTRUMCT、2D 和 3D 光学体内断层扫描系统。

• 批准号: 9907225
• 负责人: Hee-Jeong Im Sampen
• 金额: --
• 依托单位: JESSE BROWN VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2020-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9907225
• 关键词: 3-Dimensional; Advisory Committees; Alcohol abuse; Alzheimer' s Disease; Anatomy; Animal Model; Animals; Anxiety; Basic Science; Bioluminescence; Chicago; Chronic; Clinical Investigator; Clinical Trials Design; Data; Data Set; Degenerative polyarthritis; Detection; Diabetic Neuropathies; Digital Imaging and Communications in Medicine; Disease; Dose; Drug Kinetics; Drug toxicity; Ensure; Equipment; Evaluation; Fluorescence; Fluorochrome; Foundations; Funding; Generations; Goals; Grant; Image; Image Analysis; Image Enhancement; Industry; Inflammatory; Interdisciplinary Study; Intuition; Laboratories; Longitudinal Studies; Magnetic Resonance Imaging; Manufacturer Name; Medical center; Mental Depression; Metabolic Diseases; Minor; Modality; Modeling; Molecular; Motion; Multiple Sclerosis; Obesity; Optical Tomography; Optics; Orthopedics; Pain; Pain Research; Parkinson Disease; Performance; Pharmacodynamics; Pilot Projects; Positron-Emission Tomography; Procedures; Research; Research Personnel; Research Support; Scanning; Services; Sheep; Surface; System; Systems Analysis; Technical Expertise; Techniques; Technology; Three-Dimensional Image; Time; Tissue Harvesting; Tracer; Training; United States National Institutes of Health; Universities; Veterans; Work; X-Ray Computed Tomography; animal facility; biomarker identification; cherenkov imaging; complex biological systems; design; detector; experience; gastrointestinal; germ free condition; gut microbes; imaging capabilities; imaging modality; imaging software; imaging system; in vivo; in vivo imaging; in vivo imaging system; in vivo optical imaging; insight; instrument; luminescence; multidisciplinary; novel therapeutics; optical imaging; pre-clinical; pre-clinical research; programs; radiotracer; real-time images; reconstruction; research and development; screening; single photon emission computed tomography; therapy development; tomography

Project Summary/Abstract We propose to acquire an IVIS (IN VIVO IMAGING SYSTEM) SPECTRUMCT. It has multiple functions, including 2D Bioluminescence, 3D Bioluminescence/ Fluorescence Tomography, PET, CT and MRI functions for small and large animals, detection of in vivo Ca2+ influx that can be useful for pain study, and drug toxicity evaluation that is critical for identifying and developing new drugs. Presently there is no any highly sensitive tomographic optical imaging systems of this kind available to researchers in Jesse Brown VAMC to support VA-funded studies. This instrument will be the first IVIS SPECTRUMCT at the Jesse Brown VAMC, and will significantly accelerate data generation for the VA-funded studies by providing sophisticated in vivo analytical capabilities that are currently unavailable at the Jesse Brown VAMC or nearby universities. By using the new analytical in vivo imaging capabilities, we also expect to use significantly fewer animals for our proposed studies by eliminating time-course studies that require sacrificing animals followed by tissue harvest. Approximately 85% of the instrument time will be utilized by these VA-funded major users, and the remaining time will be used to support NIH-, DoD-funded or other pilot projects. This instrument will be located in the VA Animal Facility where the VA investigators can conduct in vivo imaging analyses without taking the animals out of the animal facility, which is designed to house specific pathogen free animals. Access to the instrument by the major/minor users will be ensured through the administrative oversight of an Advisory Committee appointed by the Chair of the R&D Committee. Administrative/technical service will be provided by Dr. Sampen who has imaging analyses experience for more than a decade, and by a coordinator, Dr. Natarajan Anbazhagan who has over 4 years of experience in directly operating the IVIS SPECTRUMCT system. To ensure that the equipment is operated safely and appropriately maintained with maximum efficiency, Drs. Sampen and Natarajan Anbazhagan will be further trained by the Perkin Elmer Technical team with intensive training courses.

项目总结/摘要 我们建议获取IVIS（体内成像系统）光谱。它有多 功能，包括2D生物发光，3D生物发光/荧光断层扫描，PET， 适用于小型和大型动物的CT和MRI功能，可检测体内Ca 2+内流， 用于疼痛研究和药物毒性评估，这对于识别和开发新的 毒品目前，还没有任何这种高灵敏度的断层摄影光学成像系统 Jesse Brown VAMC的研究人员可以使用它来支持VA资助的研究。该工具将 成为Jesse Brown VAMC的第一台IVIS SPECTRUMCT，并将显著加快数据传输速度 通过提供复杂的体内分析能力， 目前在杰西布朗VAMC或附近的大学不可用。通过使用新的 分析体内成像能力，我们还希望使用更少的动物， 通过消除需要牺牲动物的时间过程研究， 组织收获。大约85%的仪器时间将由这些VA资助的 主要用户，剩余时间将用于支持NIH，国防部资助或其他试点 项目该仪器将位于退伍军人管理局动物设施中，退伍军人管理局研究人员可以在那里 进行体内成像分析，而无需将动物带出动物设施， 专为无特定病原体动物而设计。主要/次要人员对仪器的使用 将通过任命一个咨询委员会进行行政监督， 由研发委员会主席主持行政/技术服务将由博士提供。 Sampen博士有十多年的成像分析经验， Natarajan Anbazhagan拥有超过4年的IVIS直接运营经验 SPECTRUMCT系统。确保设备安全、适当地运行 以最大的效率保持，Sampen和Natarajan Anbazhagan博士将进一步 由Perkin Elmer技术团队通过强化培训课程进行培训。