ShEEP Request for IVIS SPECTRUMCT, 2D and 3D Optical In Vivo Tomography System.

ShEEP 请求 IVIS SPECTRUMCT、2D 和 3D 光学体内断层扫描系统。

• 批准号: 9907225
• 负责人: Hee-Jeong Im Sampen
• 金额: --
• 依托单位: JESSE BROWN VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2020-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9907225
• 关键词: 3-Dimensional; Advisory Committees; Alcohol abuse; Alzheimer' s Disease; Anatomy; Animal Model; Animals; Anxiety; Basic Science; Bioluminescence; Chicago; Chronic; Clinical Investigator; Clinical Trials Design; Data; Data Set; Degenerative polyarthritis; Detection; Diabetic Neuropathies; Digital Imaging and Communications in Medicine; Disease; Dose; Drug Kinetics; Drug toxicity; Ensure; Equipment; Evaluation; Fluorescence; Fluorochrome; Foundations; Funding; Generations; Goals; Grant; Image; Image Analysis; Image Enhancement; Industry; Inflammatory; Interdisciplinary Study; Intuition; Laboratories; Longitudinal Studies; Magnetic Resonance Imaging; Manufacturer Name; Medical center; Mental Depression; Metabolic Diseases; Minor; Modality; Modeling; Molecular; Motion; Multiple Sclerosis; Obesity; Optical Tomography; Optics; Orthopedics; Pain; Pain Research; Parkinson Disease; Performance; Pharmacodynamics; Pilot Projects; Positron-Emission Tomography; Procedures; Research; Research Personnel; Research Support; Scanning; Services; Sheep; Surface; System; Systems Analysis; Technical Expertise; Techniques; Technology; Three-Dimensional Image; Time; Tissue Harvesting; Tracer; Training; United States National Institutes of Health; Universities; Veterans; Work; X-Ray Computed Tomography; animal facility; biomarker identification; cherenkov imaging; complex biological systems; design; detector; experience; gastrointestinal; germ free condition; gut microbes; imaging capabilities; imaging modality; imaging software; imaging system; in vivo; in vivo imaging; in vivo imaging system; in vivo optical imaging; insight; instrument; luminescence; multidisciplinary; novel therapeutics; optical imaging; pre-clinical; pre-clinical research; programs; radiotracer; real-time images; reconstruction; research and development; screening; single photon emission computed tomography; therapy development; tomography

Project Summary/Abstract We propose to acquire an IVIS (IN VIVO IMAGING SYSTEM) SPECTRUMCT. It has multiple functions, including 2D Bioluminescence, 3D Bioluminescence/ Fluorescence Tomography, PET, CT and MRI functions for small and large animals, detection of in vivo Ca2+ influx that can be useful for pain study, and drug toxicity evaluation that is critical for identifying and developing new drugs. Presently there is no any highly sensitive tomographic optical imaging systems of this kind available to researchers in Jesse Brown VAMC to support VA-funded studies. This instrument will be the first IVIS SPECTRUMCT at the Jesse Brown VAMC, and will significantly accelerate data generation for the VA-funded studies by providing sophisticated in vivo analytical capabilities that are currently unavailable at the Jesse Brown VAMC or nearby universities. By using the new analytical in vivo imaging capabilities, we also expect to use significantly fewer animals for our proposed studies by eliminating time-course studies that require sacrificing animals followed by tissue harvest. Approximately 85% of the instrument time will be utilized by these VA-funded major users, and the remaining time will be used to support NIH-, DoD-funded or other pilot projects. This instrument will be located in the VA Animal Facility where the VA investigators can conduct in vivo imaging analyses without taking the animals out of the animal facility, which is designed to house specific pathogen free animals. Access to the instrument by the major/minor users will be ensured through the administrative oversight of an Advisory Committee appointed by the Chair of the R&D Committee. Administrative/technical service will be provided by Dr. Sampen who has imaging analyses experience for more than a decade, and by a coordinator, Dr. Natarajan Anbazhagan who has over 4 years of experience in directly operating the IVIS SPECTRUMCT system. To ensure that the equipment is operated safely and appropriately maintained with maximum efficiency, Drs. Sampen and Natarajan Anbazhagan will be further trained by the Perkin Elmer Technical team with intensive training courses.

项目概要/摘要 我们建议购买 IVIS（体内成像系统）SPECTRUMCT。它有多个 功能，包括 2D 生物发光、3D 生物发光/荧光断层扫描、PET、 适用于小型和大型动物的 CT 和 MRI 功能，可检测体内 Ca2+ 流入 对于疼痛研究和药物毒性评估非常有用，这对于识别和开发新药物至关重要 药物。目前尚无此类高灵敏度断层光学成像系统 可供 Jesse Brown VAMC 的研究人员支持 VA 资助的研究。该仪器将 成为 Jesse Brown VAMC 的第一个 IVIS SPECTRUMCT，并将显着加速数据传输 通过提供复杂的体内分析能力，为 VA 资助的研究产生 目前 Jesse Brown VAMC 或附近的大学无法提供。通过使用新的 分析体内成像能力，我们还期望在我们的研究中使用显着更少的动物 拟议的研究，通过消除需要牺牲动物的时间过程研究，然后 组织收获。大约 85% 的仪器时间将由这些 VA 资助的项目使用 主要用户，剩余时间将用于支持 NIH、DoD 资助或其他试点 项目。该仪器将位于 VA 动物设施中，VA 研究人员可以 无需将动物带出动物设施即可进行体内成像分析，这是 设计用于容纳无特定病原体的动物。主修/辅修对乐器的访问 将通过任命的咨询委员会的行政监督来确保用户 由研发委员会主席主持。行政/技术服务将由博士提供。 Sampen 拥有十多年的成像分析经验，协调员 Dr. Natarajan Anbazhagan 拥有超过 4 年直接运营 IVIS 的经验 光谱CT系统。确保设备安全、正常运行 保持最高效率，博士。 Sampen 和 Natarajan Anbazhagan 将进一步 由 Perkin Elmer 技术团队进行强化培训课程培训。