Functional evolution of segmentation gene regulatory networks in insects
昆虫分段基因调控网络的功能进化
基本信息
- 批准号:9908087
- 负责人:
- 金额:$ 30.46万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2015
- 资助国家:美国
- 起止时间:2015-05-01 至 2023-04-30
- 项目状态:已结题
- 来源:
- 关键词:AnimalsAnopheles GenusArthropodsAsiansBiological ModelsBlack CurrantCellsClustered Regularly Interspaced Short Palindromic RepeatsCulicidaeDNA BindingDefectDevelopmentDrosophila genusEmbryoEmbryonic DevelopmentEssential GenesEvolutionFAIRE sequencingFamily memberFlourGene ExpressionGene Expression ProfileGene Expression RegulationGene Transfer TechniquesGenesGenetic ScreeningGenetic VariationGenomeHandHealthHemipteraHomeobox GenesHumanInsectaKnowledgeLightMaintenanceModelingMolecularMolecular BiologyMolecular EvolutionMolecular GeneticsMorphogenesisMorphologyMutationNatureNuclear ReceptorsOrder ColeopteraOrthologous GenePatternPigmentation physiologic functionPostdoctoral FellowProteinsProtocols documentationRNA InterferenceRadiationRegulationRegulator GenesRegulatory ElementRegulatory PathwayRiskSystemTechniquesTestingTranscriptional ActivationTranslational ResearchTriboliumVariantWorkbasecofactordesignexperimental studygene functiongene interactiongenetic approachgenetic evolutiongraduate studenthuman diseasein vivoinsightknock-downmalaria mosquitomembermutation screeningnovelpost-doctoral trainingprotein functiontooltool developmenttraittranscription factortranscriptome sequencingtranslational studyundergraduate studentvector
项目摘要
Abstract
A central question in the field of Evo-Devo is how genes controlling embryonic development
change during evolution. Many recent advances in Evo-Devo have identified genetic changes
that are associated with the acquisition of, or changes in, external body features, such as
alterations in pigmentation patterns or development of body armor. In contrast, our studies are
novel in this field as they have revealed unexpected genetic variation underlying a highly
conserved trait: the shared segmented body plan of insects. The genes controlling
segmentation encode transcription factors that are required for embryonic development and
viability. Thus, it has been surprising to find large differences in the presence, expression, or
function of these genes in different insect taxa. The work proposed here is designed to
understand the mechanistic changes underlying this observed genetic variation, in different
insect lineages. To carry out functional studies, we have developed molecular genetic
approaches in diverse insect species in our lab. The establishment of multiple non-model
systems simultaneously within one lab has synergistic effects due to sharing of protocols and
troubleshooting strategies, allowing us to more effectively develop new techniques in different
species. With these tools in hand, we will examine the underlying bases of specific scenarios of
regulatory gene variation: Aim 1. Re-wiring of gene regulatory interactions that permit loss of a
conserved regulator in mosquitoes; Aim 2. Stability of target gene regulation despite switches in
transcription factor partners: Ftz-F1 regulates target genes without Ftz in Tribolium. Aim 3.
Regulation of segmentation by non-canonical PRGs in Hemiptera. These studies will contribute
to our understanding of fundamental mechanisms regulating embryonic development and how
these mechanisms have changed during the radiation of insects. This project will train
postdoctoral fellows, one graduate student, and at least four undergraduate students in
molecular biology, genetics and molecular evolution. Establishment of molecular techniques in
non-model and emerging model insect species, including expression analysis, RNA
interference, CRISPR, FAIRE-seq, and transgenesis, not only allows us to answer fundamental
questions about embryonic development, but also provides molecular tools for translational
studies of insects that pose a risk to human health.
摘要
Evo-Devo领域的一个中心问题是控制胚胎发育的基因如何
在进化过程中发生变化。最近在进化发育方面的许多进展已经确定了遗传变化
与外部身体特征的获得或变化相关的,例如
色素沉着模式的改变或防弹衣的发展。相反,我们的研究
这是该领域的新发现,因为他们揭示了一种高度遗传变异的基础,
保守性状:昆虫共有的分节的身体平面。基因控制
分节编码胚胎发育所需的转录因子,
生存能力。因此,令人惊讶的是,发现在存在、表达或表达方面的巨大差异。
这些基因在不同昆虫类群中的功能。这里提出的工作旨在
理解这种观察到的遗传变异的机制变化,在不同的
昆虫谱系为了进行功能研究,我们开发了分子遗传学,
在我们的实验室里,多元非模型的建立
由于共享协议,一个实验室内同时使用的系统具有协同效应,
故障排除策略,使我们能够更有效地开发新技术,
物种有了这些工具,我们将研究
调控基因变异:目的1。基因调控相互作用的重新连接,
蚊子中的保守调节因子;目的2.靶基因调控的稳定性,尽管开关在
转录因子伴侣:Ftz-F1在赤拟谷盗中调节靶基因而不调节Ftz。目标3。
半翅目非典型PRG对卵裂的调控。这些研究将有助于
对我们理解胚胎发育的基本机制以及
这些机制在昆虫的辐射过程中发生了变化。该项目将培训
博士后研究员,一名研究生和至少四名本科生,
分子生物学、遗传学和分子进化。分子生物学技术的建立
非模式和新兴模式昆虫物种,包括表达分析,RNA
干扰,CRISPR,FAIRE-seq和转基因,不仅使我们能够回答基本的问题,
关于胚胎发育的问题,但也提供了翻译的分子工具,
对危害人类健康的昆虫的研究。
项目成果
期刊论文数量(0)
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{{ truncateString('Leslie Pick', 18)}}的其他基金
Functional evolution of segmentation gene regulatory networks in insects
昆虫分段基因调控网络的功能进化
- 批准号:
10658265 - 财政年份:2015
- 资助金额:
$ 30.46万 - 项目类别:
Functional evolution of segmentation gene regulatory networks in insects
昆虫分段基因调控网络的功能进化
- 批准号:
10386801 - 财政年份:2015
- 资助金额:
$ 30.46万 - 项目类别:
Functional evolution of segmentation gene regulatory networks in insects
昆虫分段基因调控网络的功能进化
- 批准号:
9058564 - 财政年份:2015
- 资助金额:
$ 30.46万 - 项目类别: