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Changes in viral and cellular gene expression mediated by KSHV PAN lncRNA

KSHV PAN lncRNA 介导的病毒和细胞基因表达的变化

基本信息

批准号：
9915852
负责人：
Cyprian Constance Rossetto
金额：
$ 35.66万
依托单位：
UNIVERSITY OF NEVADA RENO
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-05-06 至 2024-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9915852
关键词：
Affect Apoptosis Caspase Cell Cycle Cell Survival Cells ChIP-seq Chromatin Chromosomes Code Complex Cytomegalovirus Data Disease Dominant-Negative Mutation Environment Epigenetic Process FAIRE sequencing Gene Expression Genetic Transcription Genome Growth Herpesviridae Histones Human Human Herpesvirus 8 Immune Evasion Immune response Infection Kaposi Sarcoma Lymphoma Lytic MALAT1 gene Mammalian Cell Mediating Mediation Modification Nuclear Nuclear RNA Poly A Polycomb Recombinants Regulation Repression Role Simplexvirus Small Interfering RNA Testing Transcript Untranslated RNA Viral Viral Genes Viral Genome Virus Virus Replication Work base body cavity chromatin modification cytokine gammaherpesvirus gene repression infancy mutant programs promoter protein protein interaction reactivation from latency recruit response transcription factor viral DNA

项目摘要

PROJECT SUMMARY Kaposi's sarcoma associated herpesvirus or human herpesvirus 8 (KSHV/HHV8) is a gamma herpesvirus that is the cause of Kaposi's sarcoma and body cavity lymphomas. During lytic reactivation, KSHV produces a highly abundant nuclear localized transcript, polyadenylated nuclear RNA, PAN RNA. PAN RNA was shown to regulate viral and cellular gene expression. One mechanism used by PAN RNA involves the mediation of changes in epigenetic modifications. A recombinant BACmid defective for PAN RNA expression fails to reactivate and produce supernatant virus. This established PAN RNA as an essential long non-coding RNA (lncRNA) for virus replication and growth. lncRNAs are defined as non protein coding transcripts longer than 200 bases. In mammalian cells lncRNAs are very abundant with numbers estimated in the tens of thousands and many are associated with disease. Many lncRNAs have now been characterized and have a broad range of functions. Many act to regulate transcription through a variety of mechanisms. lncRNAs can act as regulators of transcription by directly interacting with transcription factors as well as recruiting transcription complexes to specific promoters. For herpesviruses, several lncRNAs have now been identified including KSHV PAN RNA, human cytomegalovirus (HCMV) lncRNA4.9 and herpes simplex virus (HSV-1) LATs. However the full understanding of the role of herpesvirus encoded lncRNAs in infection is in its infancy. There is a critical need to define the function of herpesvirus lncRNAs and their contribution to virus replication and affects on cell gene expression.

项目总结