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Role of tubuloglomerular feedback in the development of hypertension in diabetes

肾小球反馈在糖尿病高血压发生中的作用

基本信息

批准号：
9917816
负责人：
RUISHENG LIU
金额：
$ 59.11万
依托单位：
UNIVERSITY OF SOUTH FLORIDA
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-04-19 至 2023-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9917816
关键词：
Address Adenosine American Blood Pressure Blood Volume Cardiovascular Diseases Complications of Diabetes Mellitus Conscious Development Diabetes Mellitus Diabetic Nephropathy Diabetic mouse End stage renal failure Excretory function Exhibits Feedback Functional disorder Generations Genetic Glomerular Filtration Rate Glucose Hyperglycemia Hypertension Impairment In Vitro Injury to Kidney Insulin-Dependent Diabetes Mellitus Juxtaglomerular Apparatus Kidney Link Macula densa Measurement Measures Mediating Micropuncture Molecular Mus Natriuresis Nephrons Nitric Oxide Nitric Oxide Synthase Nitric Oxide Synthase Type I Non-Insulin-Dependent Diabetes Mellitus Oxides Patients Pharmacology Phosphorylation Population Protein Isoforms RNA Splicing Role Sodium Techniques Testing Tissues United States Variant Water arteriole base cardiovascular disorder risk cardiovascular risk factor diabetic diabetic patient fluorescence imaging in vivo knockout animal laser capture microdissection new therapeutic target non-diabetic novel pressure response solute

项目摘要

There are more than 29 million Americans suffering from diabetes. About 30-40% of patients with diabetes will eventually develop diabetic nephropathy, which is the leading cause of end stage renal disease in the United States. The rate of hypertension is more than twice in diabetic patients than the non-diabetic population. Diabetic patients with hypertension exhibit increased cardiovascular risk and exacerbation of diabetic nephropathy, however, the mechanisms for hypertension in diabetic patients remains unclear. The present proposal will test the central hypothesis that the increase of glucose concentration at the macula densa in diabetes activates SGLT1, which enhances NOS1 activity and promotes the development of glomerular hyperfiltration. Inadequate NO generation by the macula densa induces hypertension in diabetes and exacerbates diabetic kidney injury by mechanisms of limiting elevations in GFR and impairing sodium excretion. This hypothesis will be tested with the following specific aims: Aim 1. Hypothesis: Glucose at the macula densa activates SGLT1 and enhances expression and activity of macula densa NOS1 splice variants by phosphorylation of Ser1412. Blunted TGF responsiveness mediated by the macula densa NOS1 promotes the development of hyperfiltration in type 1 and type 2 diabetes. Aim 2. Hypothesis: Inadequate NO generation by the macula densa induces hypertension and exacerbates diabetic kidney injury in type 1 and type 2 diabetes. The mechanism involved is that enhanced TGF response limits the development of glomerular hyperfiltration, which impairs renal sodium excretion and pressure natriuresis in diabetes.

美国有2900多万人患有糖尿病。约30%-40%的糖尿病患者最终会发展成糖尿病肾病，这是美国终末期肾病的主要原因。各州。糖尿病患者的高血压患病率是非糖尿病人群的两倍多。糖尿病患者高血压患者心血管风险增加，糖尿病肾病加重，然而，糖尿病患者高血压的机制仍不清楚。目前的提议将检验中心假设，即黄斑部葡萄糖浓度的增加糖尿病患者Densa激活SGLT1，增强NOS1活性，促进肾小球发育超滤。致密黄斑产生的NO不足会导致糖尿病和高血压通过限制GFR升高和减少钠排泄的机制加重糖尿病肾脏损伤。这一假设将以以下具体目标进行检验：目的1.假设：致密黄斑葡萄糖激活SGLT1，增强SGLT1的表达和活性致密黄斑Ser1412的磷酸化剪接变异体。钝化的转化生长因子反应性由致密黄斑NOS1促进1型和2型糖尿病患者发生高滤过。目的2.假设：致密黄斑产生的NO不足会导致高血压并加重 1型糖尿病和2型糖尿病合并糖尿病肾损害。其中涉及的机制是增强的转化生长因子反应限制肾小球高滤过的发展，这会损害肾脏的钠排泄和压力糖尿病患者的钠尿症。