PET-EPRI

基本信息

批准号：
9916750
负责人：
RAYMOND ROBERT RAYLMAN
金额：
$ 58.59万
依托单位：
WEST VIRGINIA UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-08-08 至 2022-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9916750
关键词：
Anatomy Animals Area Biochemistry Biological Biomedical Research Cell Proliferation Characteristics Chemicals Complex Computer software Computers Cone Coupled Detection Development Diagnosis Electromagnetics Electron Spin Resonance Spectroscopy Elements Event Functional Imaging Glycolysis Goals Heart Neoplasms Human Hybrids Hyperplasia Hypoxia Image Image Enhancement Imaging technology Incidence Individual Interruption Investigation Knowledge Lead Light Magnetic Resonance Imaging Magnetism Malignant Neoplasms Maps Measurement Metabolism Metastatic breast cancer Metastatic to Methods Modality Molecular Probes Mouse Mammary Tumor Virus Mus Neoplasms Normal tissue morphology Photons Physiological Physiology Positioning Attribute Positron-Emission Tomography Process Protocols documentation Research Research Personnel Resolution Scanning Shapes Silicon Solid Standardization Structure Surface System Systems Development Systems Integration Technology Testing Time Tissues Transgenic Mice Translating Tumor Markers Tumor Tissue X-Ray Computed Tomography anatomic imaging animal imaging base carcinogenesis carcinogenicity clinical practice design design and construction detector disorder prevention experience extracellular fluorodeoxyglucose positron emission tomography image registration imager imaging modality imaging probe imaging system in vivo insight magnetic dipole magnetic field malignant breast neoplasm novel operation photomultiplier portability pre-clinical preclinical imaging seal software development tool tumor tumor microenvironment

项目摘要

The advent of hybrid scanners, combining complementary modalities, has revolutionized imaging; enhancing clinical practice and biomedical research. The standard paradigm is to combine an anatomical imaging method (X-ray CT, for example) with a functional method (PET, for example). In this project we propose a shift of this paradigm by investigating the melding of two complementary, functional imaging methods. Specifically, we plan to integrate a PET scanner with an electron paramagnetic resonance imaging (EPRI) scanner. EPRI is a relatively new method, capable of mapping the in vivo chemical characteristics of tissue. A combined PET- EPRI scanner has the promise to provide new insights into physiologic interactions in microenvironments not currently attainable with current imagers. Development of the PET/EPRI system is technically challenging, requiring unique approaches to scanner design, construction and testing. We will utilize a novel PET scanner that replaces a ring of discrete detector modules with a solid annulus of scintillator (spatial resolution= ~1mm). This design eliminates conductive material needed to construct discrete detector modules. Use of annular scintillator results in high detection sensitivity (~10%) due to elimination of gaps between discrete modules. It also permits estimation of event depth-of-interactions in the detector by correlating light cone shape with depth, a capability not possible with discrete detectors. Arrays of silicon photomultipliers (SiPM), which are not affected by the presence of magnetic fields present in EPR systems, will be used to detect scintillator light. The EPRI system will utilize the rapid scanning method to produce spatial maps of spectra obtained from molecular probes (resolution <1mm). We plan to use a nested design in which the animal handling enclosure, where the system’s RF resonator, shielding, EPR scan coils, PET scanner and gradient coils are combined into a single, compact PET-EPRI insert. The insert will be mounted on a computer-controlled gantry, permitting positioning inside the electro-dipole magnet (400G) required for EPR. The portable animal enclosure will include a grid of fiducial markers to facilitate registration with images acquired on our group’s small animal, MRI scanner. Initial testing of the PET/EPRI scanner will be performed using standardized testing protocols and phantoms emulating physiologic microenvironments. To demonstrate the potential utility of the new system, it will be used in a study of the interaction between the intra-and extracellular components of tumor microenvironments. This investigation will utilize MMTV-PyMT-transgenic mice that spontaneously-develop breast cancer. 18F-fluorodeoxyglucose (FDG)-PET imaging will be used to quantify areas of enhanced glycolysis (intracellular component), while EPR imaging, using a multifunctional trityl probe, will be used to quantify hypoxic and acidic areas (extracellular component) at extended time points as hyperplasia evolves in to metastatic breast cancer. The resulting information will help explore the hypothesis that carcinogenic progression is an evolutionary process, and illustrate the unique capabilities of a combined PET-EPRI scanner.

混合扫描仪的冒险结合了互补方式，彻底改变了成像。增强临床实践和生物医学研究。标准范式是结合解剖成像方法（例如，X射线CT）使用功能方法（例如，PET）。在这个项目中，我们提出了这一转变范式通过研究两种完整性，功能成像方法的融合。具体来说，我们计划将PET扫描仪与电子顺磁共振成像（EPRI）扫描仪集成。 EPRI是一个相关的新方法，能够映射组织的体内化学特征。一个组合的宠物 - EPRI扫描仪有望提供有关微环境中生理相互作用的新见解目前可以使用当前的Imagesr来实现。宠物/EPRI系统的开发在技术上具有挑战性，需要独特的扫描仪设计，构造和测试的方法。我们将利用一个新颖的宠物扫描仪这用闪烁器的实心环（空间分辨率= 〜1mm）代替了离散检测器模块的环。该设计消除了构建离散检测器模块所需的导电材料。使用环形由于消除离散模块之间的差距，闪烁体导致高检测灵敏度（约10％）。它还允许通过将光锥形状与深度相关联，对检测器中的事件深度进行估计，离散检测器不可能的功能。硅光电塑料阵列（SIPM），不是受EPR系统中存在的磁场的影响，将用于检测闪烁体的光。 EPRI系统将利用快速扫描方法生成从光谱的空间图分子问题（分辨率<1mm）。我们计划使用嵌套设计，其中动物处理围栏，系统的RF谐振器，屏蔽，EPR扫描线圈，PET扫描仪和梯度线圈合并进入一个紧凑的PET-EPRI插入物。该插件将安装在计算机控制的龙门上，允许 EPR所需的电偶极磁铁（400G）内部定位。便携式动物围栏将包括一个信托标记的网格，以促进在我们小组的小动物上获取的图像的注册， MRI扫描仪。 PET/EPRI扫描仪的初始测试将使用标准化测试协议进行和模拟生理微环境的幻影。展示新的实用性系统，它将用于研究肿瘤内和细胞外成分之间的相互作用微环境。这项投资将利用赞助开发的MMTV-PYMT-转基因小鼠乳腺癌。 18F氟脱氧葡萄糖（FDG）-PET成像将用于量化增强的区域糖酵解（细胞内组件），而EPR成像（使用多功能棘手探针）将用于量化时间点的低氧和酸性区域（细胞外成分），作为增生的演变转移性乳腺癌。最终的信息将有助于探讨致癌的假设进展是一个进化过程，并说明了组合宠物 - EPRI扫描仪的独特功能。