Translational Biomarker Facility Core

翻译生物标志物核心（待定）：摘要 翻译生物标志物核心（TBC）支持在 战略愿景中描述的环境毒理学卓越（CEET）。核心使用生物分析 用于测量暴露和效应、疾病发作和进展以及治疗的生物标志物的方法 对环境病原学疾病的反应。TBC采用严格的基于质谱（MS）的 在基于细胞培养的模型、动物模型以及群体和患者中鉴定生物标志物的方法 在综合健康科学设施核心（IHSFC）设计的队列研究。测量的分析物为金属 离子、小生物分子、蛋白质以及靶向和非靶向代谢组作为本发明的报告物。 “麻烦”TBC认识到需要将生物标志物的表型变化与生物标志物的表型变化相关联。 转录组或遗传变异，这是通过数据整合进行的曝光 生物信息学核心（EBIC）。基因组学、蛋白质组学和代谢组学生物标志物的整合提供了 有望提供新的诊断和预后指标。我们的目标是将发现转化为改进 我们称之为精准公共卫生 （PPH）。TBC配备了最先进的超高效液相色谱（UPLC）-MS， UPLC-高分辨率（HR）MS仪器。它还配备了电感耦合等离子体（ICP）-MS 重金属形态分析许多LC-MS测定使用稳定同位素稀释（SID）LC-MS作为测定方法。 精密度和准确度的金标准，许多测定无法在世界其他地方进行。 核心将实现以下目标。目标1：我们将提供高灵敏度和特异性的CEET检测方法 研究人员分析模型系统和人群生物流体中暴露和效应的生物标志物 队列和患者。在目标2中：我们将支持CEET的空气污染和肺部健康专题领域; 环境暴露和癌症，敏感窗口;和环境神经科学服务 并且能够使用蛋白质组学和代谢组学方法发现生物标志物。目标3：我们将开发新的 根据CEET研究人员的需求，为专题领域提供分析和优先服务。目标4：我们将 为试点项目接受者和环境卫生科学的初步研究提供分析支助。 目标5：我们将提供使用最先进的分析设备和软件的机会和培训，包括 用于金属分析的新型ICP-MS仪器。目标6：我们将把TBC服务与 EBIC和IHSFC使人类受试者研究无缝衔接;目标7：我们将促进生物标志物 通过每月培训班和年度讲习班，为CEET调查人员及其受训人员提供教育。

TRANSLATIONAL BIOMARKER CORE (TBC): ABSTRACT The Translational Biomarker Core (TBC) supports exposure assessment conducted in the Center of Excellence in Environmental Toxicology (CEET) described in the Strategic Vision. The Core uses bioanalytical methods to measure biomarkers of exposure and effect, disease onset and progression, and therapeutic response for diseases of environmental etiology. The TBC employs rigorous mass spectrometry (MS)-based methodology to identify biomarkers in cell-culture based models, in animal models and in population and patient cohorts studies designed in the Integrative Health Sciences Facility Core (IHSFC). Analytes measured are metal ions, small biomolecules, proteins, as well as targeted and untargeted metabolomes as reporters of the “exposome”. The TBC recognizes the need to relate phenotypic changes in biomarkers with either changes in the transcriptome or with genetic variants and this is achieved by data integration performed by the Exposure Biology Informatics Core (EBIC). The integration of genomic, proteomic and metabolomics biomarkers offers the promise to deliver new diagnostic and prognostic indicators. Our goal is to translate findings to improve environmental health in vulnerable populations or individuals which we call Precision Public Health (PPH). The TBC is equipped with state-of-the art ultraperformance liquid chromatography (UPLC)-MS and UPLC-high resolution (HR) MS instrumentation. It is also equipped with inductively coupled plasma (ICP)-MS for heavy metal analysis with speciation. Many LC-MS assays use stable-isotope dilution (SID) LC-MS as the gold standard for precision and accuracy and many of the assays cannot be performed elsewhere in the world. The Core will perform the following aims. In Aim 1: we will provide highly sensitive and specific assays to CEET investigators to analyze biomarkers of exposure and effect in model systems and in biofluids from population cohorts and patients. In Aim 2: we will support the CEET thematic areas in Air Pollution & Lung Health; Environmental Exposures & Cancer, Windows-of-Susceptibility; and Environmental Neuroscience with services and enable biomarker discovery using proteomics and metabolomics approaches. In Aim 3: we will develop new assays for the thematic areas and prioritize services based on CEET investigators needs. In Aim 4: we will provide analytical support for pilot-project recipients and for preliminary studies in environmental health sciences. In Aim 5: we will provide access and training to use state-of-the art analytical equipment and software, including the new ICP-MS instrument for metal analysis. In Aim 6: we will integrate the TBC services with those of the EBIC and IHSFC so that human subject research is seamless; and in Aim 7: we will facilitate biomarker education for CEET investigators and their trainees through monthly training sessions and annual workshops.