Deactivation Mechanisms of Rod Phototransduction

项目摘要 视网膜视杆细胞和视锥感光细胞通过 信号光强及其调制。虽然最初的生化步骤是转译 在光感受器的外部节段，光转化为电信号是众所周知的， 关于外段电流是如何通过电压敏感型进行转换的，我们知之甚少 电导，并受体内适应和昼夜节律的影响。这项工作 建议在这项应用中研究光感受器生理学，活眼和 光感受器生理调节以影响视觉敏感度和 动力学，并缓解光感受器压力。主要使用体外和体内的组合 活体电生理方法，将研究三个目的：1.确定其作用 Kv2.1通道，以棒为单位。2.定义限制光感受器敏感度和 体内动力学。3.确定昼夜节律是如何调节感光细胞特性的 钟。

Project Summary Retinal rod and cone photoreceptor cells mediate the essential first steps in vision by signaling light intensity and its modulation. Although the initial biochemical steps that transduce light into electrical signals in the outer segments of photoreceptors are well understood, much less is known about how outer segment currents are transformed by voltage-sensitive conductances and influenced by adaptation and circadian regulation in vivo. The work proposed in this application will investigate photoreceptor physiology the living eye and the mechanisms by which photoreceptor physiology is modulated to influence visual sensitivity and kinetics, and to mitigate photoreceptor stress. Using primarily a combination of ex vivo and in vivo electrophysiological approaches, three aims will be investigated: 1. Determine the role of Kv2.1 channels in rods. 2. Define the mechanisms that constrain photoreceptor sensitivity and kinetics in vivo. 3. Determine how photoreceptor properties are modulated by the circadian clock.