Translational Science BioCore Shared Resource

转化科学 BioCore 共享资源

• 批准号: 9923025
• 负责人: Kristina A. Matkowskyj
• 金额: $ 9.08万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9923025
• 关键词: Address; Archives; Blood; Body Fluids; Cancer Burden; Cancer Center Support Grant; Cell Line; Clinical; Clinical Data; Clinics and Hospitals; Code; Collection; Communities; Comprehensive Cancer Center; Confidentiality of Patient Information; Consult; DNA; Development; Disease; Ensure; Formalin; Freezing; Frozen Sections; Funding; Histopathology; Human; Human body; Immunohistochemistry; In Situ Hybridization; Infrastructure; Institutional Review Boards; Laboratories; Link; Mission; Molecular; Molecular Analysis; Paraffin Embedding; Pathologist; Pathology; Patients; Procedures; Proteins; Protocols documentation; RNA; Reproducibility; Research; Research Personnel; Resource Sharing; Safety; Services; Slide; Specific qualifier value; Specimen; Time; Tissue Embedding; Tissue Microarray; Tissue Stains; Tissues; Translational Research; United States National Institutes of Health; Wisconsin; biobank; clinically relevant; digital pathology; disorder subtype; human tissue; imaging platform; innovation; instrumentation; laser capture microdissection; liquid crystal polymer; meetings; member; molecular pathology; molecular phenotype; neoplastic; operation; pathology imaging; prospective; translational cancer research

PROJECT SUMMARY / ABSTRACT The mission of the Translational Science Biocore (TSB) is to facilitate basic and translational cancer research by providing UWCCC members with a comprehensive array of services that enable cellular and molecular characterization of human derived biospecimens. Aim 1 is to collect and distribute human derived biospecimens. TSB operates with Institutional Review Board (IRB) approval to prospectively collect, store, and distribute a wide variety of human tissues and body fluids from patients being treated within our hospitals and clinics. Standard Operating Procedures have been established to ensure the collected biospecimens meet specified quality parameters. TSB is authorized by the IRB to serve as an ‘honest broker’ and provide investigators with needed clinical and demographic annotations that are coded in a manner that protects patient confidentiality. TSB also provides biospecimen collection, processing, and storage services to UWCCC members who direct project specific, IRB-approved protocols. Aim 2 is to construct and distribute disease specific tissue microarrays (TMAs). TSB pathologists review archival tissue blocks to identify specimens well suited for inclusion in each disease specific TMA, which is then constructed by TSB staff. Each TMA is fully annotated with relevant clinical data and is distributed to UWCCC members following appropriate institutional review. Aim 3 is to provide molecular pathology and imaging expertise and infrastructure. TSB pathologists and expert staff consult with UWCCC members to guide development, conduct, and interpretation of studies that utilize human derived biospecimens and also provide broad expertise and services in a variety of molecular pathology applications, including morphometric analysis of stained tissue sections, quantitative analyses of specific molecular phenotypes, laser capture microdissection, in situ hybridization, and immunohistochemistry. TSB houses the state-of-the-art instrumentation required for each of these services; e.g., Vectra and Nuance multispectral imaging platforms and Aperio digital pathology whole slide scanner. TSB also provides an assortment of basic histopathology support services on human derived biospecimens. Finally, TSB provides a cell line authentication service to assist UWCCC members in meeting NIH requirements for scientific rigor and reproducibility. Impact on UWCCC: TSB has achieved many noteworthy milestones during the current CCSG funding cycle. During this period, we have increased the volumes of biospecimens collected and distributed, the numbers of protocols and trials supported, and the number of UWCCC members served. We continuously evaluate the needs of the UWCCC research community and strive to address all identified needs in a timely manner. We have enhanced our existing services and have added new services to enable our members to perform innovative research that advances the UWCCC mission.

项目总结/摘要 转化科学生物中心（TSB）的使命是促进基础和转化癌症研究 通过为UWCCC成员提供全面的服务， 人源性生物标本的表征。目标1是收集和分发人源性 生物标本TSB经机构审查委员会（IRB）批准，可前瞻性地收集、储存和 分发来自我们医院内接受治疗的患者的各种人体组织和体液， 诊所。已建立标准操作规程，以确保采集的生物标本符合 规定的质量参数。TSB被IRB授权作为“诚实的经纪人”， 研究者提供所需的临床和人口统计学注释， 病人隐私TSB还为UWCCC提供生物标本采集、处理和储存服务 指导项目特定的、IRB批准的方案的成员。目标2是构建和传播疾病 特异性组织微阵列（TMAs）。TSB病理学家审查存档组织块以很好地识别标本 适合于包含在每个疾病特定的TMA中，然后由TSB工作人员构建。每个TMA都是 注释了相关的临床数据，并在适当的机构 审查.目标3是提供分子病理学和影像学专业知识和基础设施。TSB病理学家和 专家工作人员与UWCCC成员协商，指导研究的开发、实施和解释， 利用人源性生物标本，并提供广泛的专业知识和服务，在各种分子 病理学应用，包括染色组织切片的形态测定分析， 特异性分子表型、激光捕获显微切割、原位杂交和免疫组织化学。 TSB拥有这些服务所需的最先进的仪器;例如，Vectra和Nuance 多光谱成像平台和Aperio数字病理学全切片扫描仪。TSB还提供 人类生物标本的基本组织病理学支持服务分类。最后，TSB提供了 细胞系认证服务，协助UWCCC成员满足NIH的科学严谨性要求， 再现性对UWCCC的影响：TSB在当前CCSG期间取得了许多值得注意的里程碑 融资周期。在此期间，我们增加了收集和分发的生物标本数量， 支持的方案和试验的数量，以及UWCCC成员的数量。我们不断 评估UWCCC研究社区的需求，并努力及时解决所有确定的需求 方式我们加强了现有的服务，并增加了新的服务，使我们的成员， 进行创新研究，推进UWCCC的使命。