Tumor Imaging Core

肿瘤成像核心

基本信息

批准号：
9974492
负责人：
Adrian Chrastina
金额：
$ 29.37万
依托单位：
PROTEOGENOMICS RESEARCH INSTIT/SYS/ MED
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-07-08 至 2024-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9974492
关键词：
Animals Antibodies Archives Biological Assay Caveolae Clinical Complement Data Data Analyses Data Storage and Retrieval Development Effectiveness Ensure Equipment Experimental Designs Fluorescence Goals Histologic Human Human Resources Image Image Analysis Malignant Neoplasms Modeling Neoplasm Metastasis Patients Pharmaceutical Preparations Primary Neoplasm Process Research Personnel Research Project Grants Roentgen Rays Scientist Services Solid Solid Neoplasm Stains Testing Tissues Training Tumor-Associated Process Vascular Endothelium Work X-Ray Computed Tomography animal imaging animal tissue base cancer imaging design experimental study human tissue image archival system imaging agent imaging approach imaging capabilities imaging modality in vivo intravital microscopy microscopic imaging novel novel therapeutics pre-clinical preclinical imaging single photon emission computed tomography targeted delivery time use tumor whole body imaging

项目摘要

PROJECT SUMMARY_CORE C Core C Preclinical Tumor Imaging will provide services and expertise to all PPG investigators in designing and executing imaging experiments required to meet the objectives of each project. Core personnel will: 1) assist with design and execute imaging experiments; 2) store, process, and distribute as needed, all tissues required for downstream analysis, or use in other projects; 3) assist or directly perform all animal imaging experiments needed; 4) assist or directly perform all animal and imaging data analyses and interpretation; and 5) facilitate archiving and retrieval of data. Investigators will be advised of the full potential of Core C capabilities to ensure that experimental designs fully utilize available services. Preclinical tumor imaging capabilities available include Single Photon Emission Computed Tomography combined with X-ray CT (SPECT-CT) for whole-body imaging, intravital microscopy (IVM) and static microscopy imaging. Each of our imaging modalities described is well- suited to focus on a specific aspect of antibody targeting and processing. The multiple imaging approaches provided by Core C will complement and confirm one another, and will provide a more complete analysis of targeting, delivery and processing in vivo at multiple levels. Data acquired will assist in: 1) validating AnnA1 expression by examining tissue sections using histochemical and fluorescence based assays; 2) defining and quantifying tumor targeting by hAnnA1 antibodies over time using dynamic whole-body imaging; and 3) evaluating and quantifying EC processing of hAnnA1 targeted immunocomplexes and conjugates via dynamic IVM in novel human tumor models. Core C services will help answer key questions on the distribution of AnnA1 in human tissues, on how tumors process specific probes in vivo, and how caveolae function in the tumor vascular endothelium. Data from preclinical image analysis will be used to assess the effectiveness of drugs and imaging agents for development in Projects 1 and 2, and eventual clinical utility in Project 3.

项目summary_core c 核心C临床前肿瘤成像将为所有PPG研究人员提供服务和专业知识执行成像实验以实现每个项目的目标。核心人员将：1）协助使用设计和执行成像实验； 2）根据需要存储，处理和分发所有组织用于下游分析或在其他项目中使用； 3）协助或直接执行所有动物成像实验需要； 4）协助或直接执行所有动物和成像数据分析和解释； 5）便利归档和检索数据。将告知调查人员核心C功能的全部潜力以确保实验设计充分利用可用的服务。可用的临床前肿瘤成像功能包括单光子发射计算机断层扫描与X射线CT（SPECT-CT）结合用于全身成像，插入显微镜（IVM）和静态显微镜成像。我们所描述的每个成像方式都是很好的专注于抗体靶向和加工的特定方面。多个成像方法由C核C提供将互补并确认，并将提供更完整的分析在多个级别的体内靶向，交付和处理。获取的数据将有助于：1）验证Anna1 通过基于组织化学和荧光测定法检查组织切片的表达； 2）定义和使用动态全身成像对Hanna1抗体量化Hanna1抗体的靶向； 3）通过动态评估和量化Hanna1靶向免疫复合物和偶联物的EC处理新型人类肿瘤模型中的IVM。核心C服务将有助于回答有关Anna1分发的关键问题在人体组织中，肿瘤如何处理体内特定探针以及小窝在肿瘤中的功能血管内皮。临床前图分析的数据将用于评估药物的有效性和项目1和2中开发的成像代理，以及项目3中最终的临床实用性。