Tumor Imaging Core

肿瘤成像核心

• 批准号: 10251319
• 负责人: Adrian Chrastina
• 金额: $ 29.07万
• 依托单位: PROTEOGENOMICS RESEARCH INSTIT/SYS/ MED
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-08 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10251319
• 关键词: Animals; Antibodies; Archives; Biological Assay; Caveolae; Clinical; Complement; Data; Data Analyses; Data Storage and Retrieval; Development; Ensure; Equipment; Experimental Designs; Fluorescence; Goals; Histologic; Human; Human Resources; Image; Image Analysis; Malignant Neoplasms; Modeling; Neoplasm Metastasis; Patients; Pharmaceutical Preparations; Primary Neoplasm; Process; Research Personnel; Research Project Grants; Roentgen Rays; Scientist; Services; Solid; Solid Neoplasm; Stains; Testing; Tissues; Training; Tumor-Associated Process; Vascular Endothelium; Work; X-Ray Computed Tomography; animal imaging; animal tissue; base; cancer imaging; design; effectiveness evaluation; experimental study; human tissue; image archival system; imaging agent; imaging approach; imaging capabilities; imaging modality; in vivo; intravital microscopy; microscopic imaging; novel; novel therapeutics; pre-clinical; preclinical imaging; single photon emission computed tomography; targeted delivery; time use; tumor; whole body imaging

PROJECT SUMMARY_CORE C Core C Preclinical Tumor Imaging will provide services and expertise to all PPG investigators in designing and executing imaging experiments required to meet the objectives of each project. Core personnel will: 1) assist with design and execute imaging experiments; 2) store, process, and distribute as needed, all tissues required for downstream analysis, or use in other projects; 3) assist or directly perform all animal imaging experiments needed; 4) assist or directly perform all animal and imaging data analyses and interpretation; and 5) facilitate archiving and retrieval of data. Investigators will be advised of the full potential of Core C capabilities to ensure that experimental designs fully utilize available services. Preclinical tumor imaging capabilities available include Single Photon Emission Computed Tomography combined with X-ray CT (SPECT-CT) for whole-body imaging, intravital microscopy (IVM) and static microscopy imaging. Each of our imaging modalities described is well- suited to focus on a specific aspect of antibody targeting and processing. The multiple imaging approaches provided by Core C will complement and confirm one another, and will provide a more complete analysis of targeting, delivery and processing in vivo at multiple levels. Data acquired will assist in: 1) validating AnnA1 expression by examining tissue sections using histochemical and fluorescence based assays; 2) defining and quantifying tumor targeting by hAnnA1 antibodies over time using dynamic whole-body imaging; and 3) evaluating and quantifying EC processing of hAnnA1 targeted immunocomplexes and conjugates via dynamic IVM in novel human tumor models. Core C services will help answer key questions on the distribution of AnnA1 in human tissues, on how tumors process specific probes in vivo, and how caveolae function in the tumor vascular endothelium. Data from preclinical image analysis will be used to assess the effectiveness of drugs and imaging agents for development in Projects 1 and 2, and eventual clinical utility in Project 3.

项目概要_核心 C Core C 临床前肿瘤成像将为所有 PPG 研究人员提供设计和 执行满足每个项目目标所需的成像实验。核心人员将：1）协助 设计和执行成像实验； 2) 根据需要存储、处理和分发所有所需的组织 用于下游分析或用于其他项目； 3）协助或直接进行所有动物成像实验 需要； 4）协助或直接进行所有动物和影像数据分析和解释； 5) 促进 数据的归档和检索。研究人员将被告知核心 C 功能的全部潜力，以确保 实验设计充分利用可用的服务。可用的临床前肿瘤成像功能包括 单光子发射计算机断层扫描与 X 射线 CT (SPECT-CT) 相结合进行全身成像， 活体显微镜（IVM）和静态显微镜成像。我们描述的每种成像方式都很好- 适合专注于抗体靶向和加工的特定方面。多种成像方法 Core C提供的内容将相互补充和印证，并将提供更完整的分析 体内多个水平的靶向、递送和加工。获取的数据将有助于：1) 验证 AnnA1 通过使用基于组织化学和荧光的测定法检查组织切片来表达； 2）定义和 使用动态全身成像对 hAnnA1 抗体随时间的肿瘤靶向进行量化；和 3) 通过动态评估和量化 hAnnA1 靶向免疫复合物和缀合物的 EC 处理 新型人类肿瘤模型中的 IVM。核心 C 服务将有助于解答有关 AnnA1 分发的关键问题 在人体组织中，肿瘤如何在体内处理特定探针，以及小窝如何在肿瘤中发挥作用 血管内皮。临床前图像分析的数据将用于评估药物的有效性和 用于项目 1 和 2 中开发的显像剂，以及项目 3 中的最终临床应用。