Red and Far-Red Fluorescent Biosensors for Compartmentalized Redox Signaling
用于分隔氧化还原信号传导的红色和远红荧光生物传感器
基本信息
- 批准号:9975915
- 负责人:
- 金额:$ 37.84万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2018
- 资助国家:美国
- 起止时间:2018-09-05 至 2022-06-30
- 项目状态:已结题
- 来源:
- 关键词:AgingAnimal ModelBasic ScienceBiologyBiosensorCancer EtiologyCell CompartmentationCell NucleusCell modelCellsChemicalsColorCoupledCouplingCytosolDetectionDevelopmentDiseaseDoxorubicinDrug ScreeningEndoplasmic ReticulumEngineeringEpidermal Growth FactorFDA approvedFluorescenceGlutathioneGoalsHumanHydrogen PeroxideImageImaging DeviceIndividualInflammatoryInterventionLaboratoriesLeadLibrariesLightMeasurementMeasuresMethodsMitochondriaMolecularMonitorNitrogenNuclearOncologyOrganismOxidation-ReductionOxidative StressOxygenPenetrationPharmaceutical PreparationsPhosphotransferasesPhotonsPhysiologicalProcessProtein Disulfide IsomeraseProteinsRegulationReportingResearchSensorySignal TransductionStimulusSulfhydryl CompoundsSulfurTXN geneTechnologyTissuesToxicologyWorkabsorptionanimal imagingbasebiomaterial compatibilitychemotherapymultiplexed imagingmutantnovelnovel therapeuticsoxidationpublic health relevanceratiometricred fluorescent proteinresponsesensorspatiotemporalsubcellular targetingtechnology research and developmenttooltoxicanttranslational studywhole animal imaging
项目摘要
ABSTRACT
Redox biology has been increasingly recognized as one of the key themes in cell signaling. A major
obstacle in this field is the lack of capabilities to monitor and measure clearly defined redox processes in live
cells (including subcellular domains in live cells), tissues, or organisms. The goal of this 4-year project,
submitted in response to PAR-17-045 “Focused Technology Research and Development”, is to expand the
fluorescent protein toolbox for red and far-red fluorescent biosensors that can be used to image
compartmentalized, thiol-based redox signaling in live cells and organisms.
In this project, we aim to advance the fluorescent biosensor technology in the following aspects : (1) we
will expand fluorescent-protein-based biosensors for additional redox parameters, including the redox of
thioredoxin and protein disulfide isomerase (PDI) in various cell compartments, such as the nucleus, cytosol,
mitochondria, and endoplasmic reticulum (ER); (2) we will expand the colors of fluorescent-protein-based
redox biosensors to red and far-red; (3) we will validate these new biosensors and demonstrate the use of
fluorescent-protein-based biosensors in compatible colors for multicompartment/multiplex imaging.
Because UV or blue light, which can induce intracellular oxidation, is not needed to excite red and far-
red fluorescent biosensors, our work will increase the biocompatibility of fluorescent-protein-based redox
biosensors. Furthermore, red and far-red biosensors will assist in whole-animal imaging, since compared to
green ones, they reduce tissue absorption and scattering.
This project will lead to important imaging tools that may accelerate the understanding of redox
regulation and signaling. As with the transformative impact of fluorescent-protein-based kinase sensors, we
expect that these redox biosensors will enable and transform a large array of basic and translational studies.
摘要
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Huiwang Ai其他文献
Huiwang Ai的其他文献
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{{ truncateString('Huiwang Ai', 18)}}的其他基金
Fluorescent Indicators for Imaging Synaptic Zinc in Cortical Sound Processing
用于皮质声音处理中突触锌成像的荧光指示剂
- 批准号:
10709627 - 财政年份:2022
- 资助金额:
$ 37.84万 - 项目类别:
Fluorescent Indicators for Imaging Synaptic Zinc in Cortical Sound Processing
用于皮质声音处理中突触锌成像的荧光指示剂
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10599603 - 财政年份:2022
- 资助金额:
$ 37.84万 - 项目类别:
An Integrated Fluorescent Biosensor, Imaging, and Analysis Toolkit for Islet Biologics
用于胰岛生物制品的集成荧光生物传感器、成像和分析工具包
- 批准号:
10613923 - 财政年份:2020
- 资助金额:
$ 37.84万 - 项目类别:
An Integrated Fluorescent Biosensor, Imaging, and Analysis Toolkit for Islet Biologics
用于胰岛生物制品的集成荧光生物传感器、成像和分析工具包
- 批准号:
10379407 - 财政年份:2020
- 资助金额:
$ 37.84万 - 项目类别:
An Integrated Fluorescent Biosensor, Imaging, and Analysis Toolkit for Islet Biologics
用于胰岛生物制品的集成荧光生物传感器、成像和分析工具包
- 批准号:
10200034 - 财政年份:2020
- 资助金额:
$ 37.84万 - 项目类别:
Red and Far-Red Fluorescent Biosensors for Compartmentalized Redox Signaling
用于分隔氧化还原信号传导的红色和远红荧光生物传感器
- 批准号:
10222722 - 财政年份:2018
- 资助金额:
$ 37.84万 - 项目类别:
BapaFPs: Genetically encoded fluorescent indicators to image live-cell nucleotide sugars
BapaFP:基因编码的荧光指示剂,用于对活细胞核苷酸糖进行成像
- 批准号:
9750256 - 财政年份:2018
- 资助金额:
$ 37.84万 - 项目类别:
Lentiviral Systems for Controlled Mammalian Expression of Unnatural Fluorescent Protein Probes
用于非天然荧光蛋白探针的受控哺乳动物表达的慢病毒系统
- 批准号:
8873037 - 财政年份:2015
- 资助金额:
$ 37.84万 - 项目类别:
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