Cell Biology and Bioimaging Core

代谢疾病领域的前沿研究需要获得现代成像、血流动力学和血液动力学。 流式细胞术和标本制备方法学。COBRE细胞生物学和生物成像 核心（CBB核心）是拟议的第三阶段核心中的一个综合组成部分- 转基因学、基因组学、细胞生物学和生物成像。CBB核心为调查人员提供 获得在细胞和组织水平上进行治疗所需的仪器、培训和专业知识 营养调节或遗传操纵的肥胖，糖尿病， 或代谢综合征的其他病理。CBB核心使初级和高级 教师探索新的方法，包括定量组织学和组织特异性 通过激光显微切割进行转录谱分析，以提高其在 缩减联邦资金Core目前的功能包括：宽视场和共焦 固定和活标本成像，全内反射荧光（TIRF），荧光 共振能量转移（FRET），光漂白后荧光恢复（FRAP），整体 载玻片扫描、分析和制备流式细胞术、激光显微切割和组织学 包括组织处理、包埋、切片和染色的服务-常规， 特殊和免疫组化。核心不断适应不断变化的需求， COBRE调查员通过频繁的自我评估和外部顾问的监督 委员会和拟议的由方案协调员领导的内部指导委员会， 博士克劳德·布沙尔核心方案的可持续性很可能是由于高水平的承诺 PBRC管理部门以及COBRE调查人员数量的增加， 获得校外资助。CBB核心目标是：1）提供具有成本效益的样本 制备和产生高质量的生物成像和流式细胞术数据; 2）为了提供访问， 培训，并与CBB核心平台的援助，与应用CBB核心平台的专业知识相结合， 必要的软件算法需要产生最先进的数据分析，解释， 3）促进新技术、新工艺的开发和实验 需要采取措施提高COBRE教师在校外资金方面的竞争力。

Cutting edge research in the field of metabolic disease requires access to modern imaging, flow cytometry, and specimen preparation methodology. The COBRE Cell Biology and Bioimaging Core (CBB Core) is an integrative component within the proposed Phase III cores – Transgenics, Genomics, and Cell Biology and Bioimaging. The CBB Core provides investigators access to the instrumentation, training, and expertise needed to conduct cellular and tissue level phenotyping of nutritionally-modulated or genetically-manipulated models of obesity, diabetes, or other pathologies of metabolic syndrome. The CBB Core enables both junior and senior level faculty to explore novel approaches that include quantitative histology and tissue specific transcript profiling via laser microdissection to increase their competitiveness in an era of shrinking federal funding. The Core's current capabilities include: widefield and confocal imaging of fixed and live specimens, total internal reflection fluorescence (TIRF), fluorescent resonant energy transfer (FRET), fluorescence recovery after photobleaching (FRAP), whole slide scanning, analytical and preparative flow cytometry, laser microdissection, and histology services that encompass tissue processing, embedding, sectioning, and staining – routine, special, and immunohistochemical. The Core continually adapts to the changing needs of the COBRE investigators through frequent self-assessments and oversight by the External Advisory Committee and the proposed Internal Steering Committee headed by the Program Coordinator, Dr. Claude Bouchard. The sustainability of the Core is likely due to the high level of commitment by the PBRC Administration as well as increases in the number of COBRE investigators that will receive extramural funding. The CBB Core aims are: 1) To provide cost effective specimen preparation and produce high quality bioimaging and flow cytometry data; 2) To provide access, training, and assistance with CBB Core platforms, in concert with expertise in application of the requisite software algorithms needed to produce state-of-the art data analysis, interpretation, and presentation; 3) To promote development new techniques, technologies, and experimental approaches needed to enhance COBRE faculty competitiveness for extramural funding.