Protein Localization, Identification and Folding Core

蛋白质定位、鉴定和折叠核心

• 批准号: 9978788
• 负责人: Bishr Omary
• 金额: $ 15.59万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9978788
• 关键词: AGTR2 gene; ART protein; Advisory Committees; Antibodies; Area; Award; Bioinformatics; Biological Process; Cellular biology; Collaborations; Confocal Microscopy; Diabetes Mellitus; Digestive System Disorders; Dimensions; Disease; Electron Microscopy; Endoplasmic Reticulum; Ensure; Equipment; Evaluation; Evolution; Flow Cytometry; Funding; Gastrointestinal tract structure; Genetic Models; Grant; Hepatitis; Human Resources; Image; Image Analysis; Imaging Techniques; Individual; Inflammatory Bowel Diseases; Investments; Label; Laboratories; Laser Scanning Confocal Microscopy; Leadership; Libraries; Link; Liver; Liver diseases; Mass Spectrum Analysis; Medicine; Michigan; Microscope; Morphology; National Institute of Diabetes and Digestive and Kidney Diseases; Organelles; Pancreatitis; Pathogenesis; Pathology; Peer Review; Physiology; Post-Translational Protein Processing; Procedures; Protein-Folding Disease; Proteins; Proteomics; Publications; Reagent; Reapplication; Research; Research Personnel; Resolution; Resources; Rodent; Services; Specimen; Stress; Structure; Surveys; System; Techniques; Three-Dimensional Image; Tissue Banks; Tissue Stains; Training; United States National Institutes of Health; Universities; base; cellular imaging; digital pathology; experience; fluorescence imaging; gastrointestinal; high resolution imaging; image processing; image reconstruction; imaging software; innovation; instrumentation; laser capture microdissection; mass spectrometer; medical schools; meetings; member; metabolomics; microbiome; mouse genetics; programs; protein folding; protein misfolding; ranpirnase; recruit; whole slide imaging

The objectives of the Protein Localization, Identification and Folding (PLIF) Core is to connect investigators to imaging techniques, particularly confocal and electron microscopy, highly specialized mass spectrometry and the new area of protein folding as related to digestive diseases. This Core has evolved since the origin of the GI Center in 1986, when it was a morphology core, to one encompassing newer and more sophisticated techniques of protein identification and localization. In the last funding period that began in 2010, the Core has been known as the Protein Identification and Localization Core. The new proposed PLIF Core has added a more robust and state-of-the-art mass spectrometry facility that is part of an institutionally funded Protein Folding Diseases Initiative at the University of Michigan. Given that multiple GI diseases, including hepatitis, inflammatory bowel diseases and pancreatitis, involve abnormalities of protein folding resulting in endoplasmic reticulum and other organelle stress, this adds another new dimension to the Core and takes advantage of significant institutional investments. The specific aims of the PLIF Core are: (1) Provide state of the art protein localization and identification facilities. Core programs include: Imaging, Proteomics and Protein Folding Diseases Initiative resources; (2) Ensure delivery of high quality services and products and provide technical oversight of all PLIF services; and (3) Train and educate members, associate members and pilot feasibility recipients in the application and use of protein identification techniques for the study of digestive and liver diseases. The most popular Core service is access to well-maintained confocal microscopes and software for image processing and 3-D image reconstruction. Other broadly used services include the evaluation of fine structure by electron microscopy and the identification of proteins and their post-translational modifications by mass spectrometry both of which are carried out by expert Core personnel. Other added aspects are an Aperio AT2 scanner and software for image analysis of stained tissue sections, and a laser capture microdissection system. The Core is directed by Drs. Asma Nusrat and Bishr Omary, two experienced and established investigators with extensive expertise in the services provided by the Core. During the current cycle of the grant, the Protein Identification and Localization Core services were used by 76% of Center members; and supported 116 publications with primary PLIF Core usage, of which 48 were collaborative publications among two or more Center members. We anticipate a similar fraction of Center members, including pilot awardees, to make use of the expanded PLIF Core. We also expect to effectively partner with the other Cores of the Center and with other NIH-supported centers, and to continue to provide state-of-the-art and efficient services and promote collaborations to synergistically advance GI research and discoveries.

蛋白质定位、鉴定和折叠（PLIF）核心的目标是连接 研究人员对成像技术，特别是共聚焦和电子显微镜，高度专业化的质量 光谱学和蛋白质折叠的新领域与消化系统疾病有关。这个核心已经进化了， 1986年GI中心的起源，当时它是一个形态学核心，到一个包含更新和更多的 复杂的蛋白质鉴定和定位技术。在2010年开始的上一个融资期， 该核心被称为蛋白质鉴定和定位核心。新的PLIF核心 增加了一个更强大和最先进的质谱仪设施，这是一个机构资助的一部分， 密歇根大学的蛋白质折叠疾病计划。鉴于多种胃肠道疾病，包括 肝炎、炎性肠病和胰腺炎，涉及蛋白质折叠异常， 内质网和其他细胞器的压力，这增加了另一个新的层面的核心，并采取 重大机构投资的优势。PLIF核心的具体目标是：（1）提供 本领域的蛋白质定位和鉴定设施。核心项目包括：成像，蛋白质组学和 蛋白质折叠疾病倡议资源;（2）确保提供高质量的服务和产品， 对所有PLIF服务提供技术监督;以及（3）培训和教育成员、准成员和 中试可行性受体在消化道蛋白质鉴定技术的应用和使用研究 和肝脏疾病。最受欢迎的核心服务是获得维护良好的共聚焦显微镜， 图像处理和三维图像重建软件。其他广泛使用的服务包括 通过电子显微镜评价精细结构和鉴定蛋白质及其翻译后 通过质谱法进行修改，这两种方法都是由核心人员进行的。其他添加的 一些方面是用于染色组织切片的图像分析的Aperio AT 2扫描仪和软件，以及激光扫描仪。 捕获显微切割系统。核心由Asma Nusrat和Bishr Omary博士执导，两位经验丰富的 并建立了在核心提供的服务方面具有广泛专门知识的调查人员。期间 在当前的资助周期中，蛋白质鉴定和定位核心服务的使用率为76%， 支持了116篇主要使用PLIF Core的出版物，其中48篇是 两个或多个中心成员之间的协作出版物。我们预计， 中心成员，包括试点获奖者，利用扩大PLIF核心。我们也期望 有效地与中心的其他核心和其他NIH支持的中心合作， 继续提供最先进和有效率的服务，并促进合作，以协同 推进GI研究和发现。