Biodegradable Matrices for Bone Healing

用于骨愈合的可生物降解基质

• 批准号: 9987102
• 负责人: Sangamesh Gurappa Kumbar
• 金额: $ 8.27万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-11-01 至 2021-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9987102
• 关键词: 3-Dimensional; Allografting; Architecture; Autologous Transplantation; Bone Growth; Bone Marrow; Bone Matrix; Bone Regeneration; Bone Tissue; Bone Transplantation; Buffers; Calcium; Calvaria; Cell Adhesion; Cell Differentiation process; Cell Proliferation; Cells; Clinic; Clinical; Defect; Deposition; Development; Event; Geometry; Glycolates; Growth Factor; Human; Implant; In Situ; In Vitro; Infiltration; Ions; Light; Mechanics; Mediating; Mesenchymal Stem Cells; Minerals; Modeling; Natural regeneration; Nutrient; Orthopedic Procedures; Orthopedics; Oryctolagus cuniculus; Osteoblasts; Osteogenesis; Performance; Phase; Phenotype; Polymers; Porosity; Production; Property; Publications; Rattus; Reconstructive Surgical Procedures; Research; Research Project Grants; Site; Solid; Structure; Surface; System; Testing; Time; Tissue Engineering; Tissues; Transplanted tissue; Vascularization; Vertebral column; Weight-Bearing state; base; biomaterial compatibility; bone; bone healing; bone repair material; calcium phosphate; clinical application; cortical bone; craniofacial; design; implantation; improved; in vivo; innovation; mechanical properties; osteoblast differentiation; poly(lactic acid); polycaprolactone; programs; reconstruction; repaired; sample fixation; scaffold; skeletal; skeletal disorder; stem cell differentiation; subcutaneous; substantia spongiosa; success; ulna

Project Summary/Abstract: Biodegradable Matrices for Bone Healing More than 6.5 million orthopaedic procedures require the use of grafts to repair bone defects every year in the US alone. Repair of large bone defects is a challenging problem in reconstructive surgery. Several bone graft options including autografts, allografts, and biodegradable porous scaffolds have been routinely used in the clinics with limited success. For example, tissue ingrowth is limited to the surface in bone tissue engineering scaffolds (BTE) because of poor access to cells and nutrients within scaffolds. The porosity that is necessary to support tissue ingrowth in BTE scaffolds results in sub-optimal mechanical properties of these materials for orthopedic applications. Therefore, there is a need to develop BTE scaffolds which will fulfill the requirements of both porosity necessary for tissue ingrowth and vascularization, and optimal mechanical properties necessary for load bearing. In addition, osteoconductive, osteoinductive, and osteointegrative properties will improve the success of bone graft materials. Our ongoing studies and publications have demonstrated the feasibility of developing mechanically strong non-porous composite scaffolds from materials with differential degradation profiles that result in the progressive formation of interconnected pores within the composite material allowing tissue ingrowth over a period of time (1, 2). Likewise, using spirally structured scaffolds we have shown that geometry can be designed to promote cell proliferation, infiltration, and homogenous mineralized matrix deposition throughout the scaffold architecture (3-6). These mechanically stable initially non-porous scaffolds were able to support bone ingrowth due to evolving porous architecture via matrix degradation in rat calvarial defects and rabbit segmental bone defects without inclusion of growth factors and cells. Based on these findings we hypothesize that by altering scaffold composition and geometry we will be able to create BTE scaffolds with programmable mechanical strength and porous structure to better serve the bone healing requirements at load bearing sites. The research project will have the four following phases: Aim 1: To optimize material composition and geometry to achieve necessary mechanical stability and progressive degradation for load-bearing bone-healing applications. Aim 2: To understand the effect of scaffold degradation on human bone marrow derived mesenchymal stem cell (MSCs) adhesion, infiltration, proliferation, differentiation, and mineralized matrix production. Aim 3: To assess in vivo biocompatibility and dynamic pore formation within the scaffolds. Aim 4: To evaluate the bone healing ability of scaffolds with different geometry and mechanical strength in a critical size segmental defect in the rabbit ulna.

项目摘要/摘要：用于骨愈合的可生物降解基质

项目成果

Nanomaterials/Nanocomposites for Osteochondral Tissue.

• DOI: 10.1007/978-3-319-76711-6_4
• 发表时间: 2018
• 期刊: Advances in experimental medicine and biology
• 作者: Ohan S. Manoukian;Connor Dieck;T. Milne;C. Dealy;Swetha Rudraiah;S. Kumbar

Decellularized extracellular matrix biomaterials for regenerative therapies: Advances, challenges and clinical prospects.

• DOI: 10.1016/j.bioactmat.2023.09.017
• 发表时间: 2024-02
• 期刊: Bioactive materials
• 影响因子: 18.9

Amorphous silica fiber matrix biomaterials: An analysis of material synthesis and characterization for tissue engineering.

• DOI: 10.1016/j.bioactmat.2022.04.002
• 发表时间: 2023-01
• 期刊: BIOACTIVE MATERIALS
• 影响因子: 18.9
• 作者: Kim, Hyun S.;Kumbar, Sangamesh G.;Nukavarapu, Syam P.
• 通讯作者: Nukavarapu, Syam P.

Fabrication of polylactic acid (PLA)-based porous scaffold through the combination of traditional bio-fabrication and 3D printing technology for bone regeneration.

• DOI: 10.1016/j.colsurfb.2020.111420
• 发表时间: 2021-01
• 期刊: Colloids and surfaces. B, Biointerfaces
• 作者: Zhou X;Zhou G;Junka R;Chang N;Anwar A;Wang H;Yu X
• 通讯作者: Yu X

Polymeric ionically conductive composite matrices and electrical stimulation strategies for nerve regeneration: In vitro characterization.

聚合物电离导电复合矩阵和神经再生的电刺激策略：体外表征。

• DOI: 10.1002/jbm.b.34272
• 发表时间: 2019-08
• 期刊: Journal of biomedical materials research. Part B, Applied biomaterials
• 作者: Manoukian OS;Stratton S;Arul MR;Moskow J;Sardashti N;Yu X;Rudraiah S;Kumbar SG
• 通讯作者: Kumbar SG