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ICAL: Impact of Cannabinoids Across Lifespan: Animal Core

ICAL：大麻素对整个生命周期的影响：动物核心

基本信息

批准号：
10188475
负责人：
Stephen Vincent Mahler
金额：
$ 51.48万
依托单位：
UNIVERSITY OF CALIFORNIA-IRVINE
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-07-01 至 2023-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10188475
关键词：
Adolescence Adolescent Adult Aftercare Age Animals Architecture Bar Codes Behavior Behavioral Biocompatible Materials Brain Cannabinoids Catalogs Cognition Collaborations Cre driver Data Databases Dopamine Dose Electrophysiology (science)Ensure Epigenetic Process Equipment Exposure to Fostering Genetic Processes Goals Individual Injections Intervention Long-Term Effects Longevity Methodology Molecular Molecular Structure Motivation Mus National Institute of Drug Abuse Neuronal Plasticity Neurons Pattern Peripheral Pharmacology Phenotype Physiological Play Procedures Protocols documentation Proxy Rattus Regimen Research Research Personnel Resources Role Sampling Scientist Standardization Sum Synapses System THC exposure Techniques Testing Tissue Banks Tissue Sample Tissues Training Transgenic Organisms Treatment Protocols Viral Vector Virus behavior test cannabinoid drug computerized designer receptors exclusively activated by designer drugs endocannabinoid signaling endogenous cannabinoid system experimental study indexing instrumentation laboratory experience marijuana use motivated behavior neural circuit pharmacokinetics and pharmacodynamics repository sex success synaptic function web site

项目摘要

PROJECT SUMMARY: ANIMAL CORE The primary goal of the Animal Core is to provide a standardized, consistent supply of adolescent and adult mice and rats of both sexes treated with THC or its vehicle for use by all ICAL projects. The Core’s trained staff will schedule treatments, behavioral testing and tissue collection, and will administer THC to animals at various ages, treatment regimens, and doses. After treatment and washout, animals will be transferred to the labs of individual ICAL projects (Molecular, Synaptic, and Behavioral). A second objective of the Animal Core is to facilitate consistent and well-validated chemogenetic (DREADD) circuit-control methodologies in ICAL projects. The Core will provide space and instrumentation (stereotaxic frame, virus injection equipment) for viral vector injection, and will train laboratory staff on procedures and protocols to ensure consistent implementation. The core will also maintain and distribute colonies of transgenic Cre driver mouse and rat lines, enabling chemogenetic manipulations of targeted neuronal subtypes including dopamine (TH:Cre or DAT:Cre), as described in individual projects. The third objective of the Animal Core is to establish and maintain an annotated repository of biological material obtained from animals treated in adolescence or adulthood with THC or vehicle. Biomaterial (brain peripheral tissues, fecal matter) from various ICAL activities (pharmacokinetics and pharmacodynamics studies, phenotyping studies, projects) will be collected, catalogued using a computerized bar-coding system and stored at -80oC. An annotated database describing available samples and associated behavioral/physiological data will be created and made available upon request to qualified researchers worldwide. This unique resource, the first in its type, will (i) foster new collaborations between ICAL and diverse research groups around the world; and (ii) open up adolescent cannabinoid research to study by groups unequipped to perform similar treatment regimens themselves. In sum, the Animal Core will be essential to ICAL’s success and broader impact. The Core will develop and implement fully characterized, consistent, and pharmacologically relevant adolescent THC exposure regimens for use in each project, and standardize use of chemogenetic methodologies across projects. The Core will also generate, catalog, and distribute tissue from these valuable animals, opening this large and rich resource to scientists around the world. !

项目总结：动物核心