Role of IL-22/IL-22RA2 during pneumococcal pneumonia

此应用程序是K 01指导职业发展奖，以促进教师的多样性， 生物医学研究。Trevejo-Nunez博士将研究IL-22/ IL-22 RA 2在肺炎球菌感染中的作用。 肺炎在美国，肺炎球菌肺炎（由S.肺炎）账户 发病率和死亡率都很高。最脆弱的人群是年龄小于 2岁以上和65岁以上的人。 该候选人的数据显示，肝脏中的IL-22信号传导对于控制肺炎球菌感染是重要的。 用细胞因子IL-22治疗可改善细菌负荷。总的假设是 通过治疗性施用IL-22或不存在诱饵， 受体IL-22 RA 2通过维持STAT 3的水平而有利于细菌清除和宿主存活 在肺炎球菌性肺炎期间肺上皮和肝脏中的活化。为了验证这一假设， 候选人提出：1）确定肺组织中内源性诱饵受体IL-22 RA 2的肺细胞来源， 未感染宿主和肺炎球菌肺炎期间。2)测试增强IL-22 RA 1 靶组织中的信号传导控制肺炎球菌肺炎期间的细菌负荷。3)测试预测 增强的IL-22信号转导导致肺中持续的STAT-3激活，而不依赖于gp 130/SOCS 3， 上皮和肝脏。 Trevejo-Nunez博士是哥伦比亚大学儿科博士后助理/医学讲师。 匹兹堡医学院。她提出了一个职业发展计划，重点是：一对一 每周与她的导师杰伊·科尔斯博士会面，学习使用转基因和 条件性基因敲除小鼠，参加学术课程以加强她在免疫学方面的基础， 统计，实验设计和赠款写作;参与负责任地开展研究讲习班， 每6个月与其审查咨询委员会举行一次会议，参加会议和出版 手稿作为第一作者。Trevejo-Nunez博士的长期目标是成为一名独立的医生- 在免疫学和宿主对S.肺炎性肺部感染。 候选人还计划收集足够的信息和数据分析，以便在最后申请R 01资金 这个奖项。

This application is for a K01 Mentored Career Development Award to Promote Faculty Diversity in Biomedical Research. Dr. Trevejo-Nunez will investigate the role of IL-22/ IL-22RA2 during pneumococcal pneumonia. In United States, pneumococcal pneumonia (lung infection caused by S. pneumoniae) accounts for significant percentage of morbidity and mortality. The most vulnerable population are children younger than 2 years old and individuals older than 65. The candidate has data that shows IL-22 signaling in the liver is important for control of pneumococcal pneumonia and that treatment with the cytokine IL-22 improves bacterial burden. The overall hypothesis is that enhancement of IL-22 signaling, through therapeutic administration of IL-22 or absence of decoy recptor IL-22RA2, favors bacterial clearance and host survival by sustaining the levels of STAT3 activation in lung epithelium and liver during in pneumococcal pneumonia. To test this hypothesis, the candidate proposes 1) Define the lung cellular sources of endogenous decoy receptor IL-22RA2 in the uninfected hosts and during pneumococcal pneumonia. 2) Test the prediction that enhanced IL-22RA1 signaling in target tissues controls bacterial burden during pneumococcal pneumonia. 3) Test the prediction that enhanced IL-22 signaling results in sustained STAT-3 activation imdependent of gp130/SOCS3 in lung epithelium and liver. Dr. Trevejo-Nunez is Postdoctoral Associate of Pediatrics/ Instructor of Medicine at the University of Pittsburgh School of Medicine. She proposes a career development plan that focuses on: one- to- one meetings with her mentor Dr. Jay Kolls every week, learning mouse model of infection using transgenic and conditional knockout mice, attending academic coursework to strengthen her foundation in immunology, statistics, experimental design and grant writing; participating in responsible conduct of research workshops, meeting with her review advisory committee every 6 months, attending conferences and publishing manuscripts as first author. Dr. Trevejo-Nunez's long term goal is to become an independent physician- scientist with particular expertise in the immunology and host response against S. pneumoniae lung infection. The candidate also plans to gather enough information and analysis of data to apply for R01 funding at the end of this award.