权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Bioimaging core

生物成像核心

基本信息

批准号：
10198696
负责人：
LUKE D HANDKE
金额：
$ 19.74万
依托单位：
UNIVERSITY OF NEBRASKA MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-07-01 至 2024-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10198696
关键词：
Affect Animals Applications Grants Architecture Cells Characteristics Clinical Complex Confocal Microscopy Core Facility Core Grant Development Developmental Process Disease Ensure Environment Event Funding Gene Expression Gene Expression Profile Genetic Transcription Genus staphylococcus Goals Heterogeneity Human Resources Image Imaging technology Immune Immune response In Vitro Infection Intervention Laboratories Life Style Medical Device Metabolic Metabolism Microbial Biofilms Microfluidics Microscope Microscopic Monitor Mus Mutation Nature Pattern Physiological Process Program Research Project Grants Regulation Reproducibility Research Role Staphylococcus aureus Staphylococcus aureus infection Structure System Techniques Technology Testing Time Tissues Tracer Virulence Virulence Factors Work acute infection base bioimaging chronic infection cost experimental study fitness implantation in vivo in vivo imaging system insight instrument instrumentation knowledge base migration novel programs

项目摘要

The remarkable ability of Staphylococcus aureus to cause acute infections can be largely attributed to the wide array of virulence factors it produces, combined with its ability to exploit various metabolic niches within a host. In contrast, its ability to cause more persistent infections is a function of its propensity to form biofilm, a process that is enhanced by the implantation of medical devices. The current funding cycle has revealed remarkable developmental processes that occur during biofilm formation, including multiple developmental stages, stochastic gene expression, and the formation of distinct structures. Based on these preliminary results, it is hypothesized that the distinct developmental stages of S. aureus biofilm development are required for optimal fitness during infection. This hypothesis will be tested in three specific aims including to 1) define the steps involved in the transition from a planktonic to sessile lifestyle, 2) provide mechanistic insight into the bistable switch, and 3) demonstrate the in vivo role of bistability. Combined, these aims will provide critical insight into the developmental steps involved in S. aureus biofilm formation and define the relevance of these processes in vivo. A common theme of this project will be the highly synergistic nature of the experiments in the context of the other projects of this program, particularly the experiments probing the regulation of virulence gene expression (Project 3) and the in vivo relevance of this regulation (Project 4). Once completed, the results of these experiments will greatly enhance our understanding of S. aureus biofilm development and provide insight into the characteristics of these complex structures that make them so recalcitrant to clinical intervention.

金黄色葡萄球菌引起急性感染的显著能力可在很大程度上