Patterning the Vertebrate Dentition Through Replacement and Repair

通过更换和修复来塑造脊椎动物的牙列

• 批准号: 10356805
• 负责人: Jeffery Todd Streelman
• 金额: $ 53.73万
• 依托单位: GEORGIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-15 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10356805
• 关键词: Adrenergic Agents; Affect; Age; Back; Biological Assay; Biology; Biomedical Engineering; Cadherins; Cells; Cichlids; Data; Defect; Dental; Dental Implants; Dentistry; Dentition; Developmental Biology; Embryo; Epithelial; Exhibits; Fishes; Genetic Diseases; Goals; Growth; Homeostasis; Human; Incisor; Knowledge; Label; Mammals; Mediating; Mesenchymal; Mesenchyme; Modeling; Molecular; Mus; Natural regeneration; Nerve; Nucleosides; Oral; Oropharyngeal; Pathway interactions; Pattern; Persons; Pharmacology; Physiologic pulse; Population; Problem Solving; Process; Recipe; Reporter; Reporting; Research; Signal Pathway; Signal Transduction; Stem Cell Factor; System; Taste Buds; Taste Perception; Testing; Tissues; Tooth Germ; Tooth Tissue; Tooth regeneration; Tooth structure; Translating; Vertebrates; WNT Signaling Pathway; World Health Organization; Zebrafish; comparative; deciduous tooth; human old age (65+); improved; innovation; insight; mutant; notch protein; permanent tooth; receptor; recruit; regenerative; regenerative biology; repaired; single-cell RNA sequencing; stem cells; tissue regeneration; transcriptome

Project Summary/Abstract The broad goal of the proposed research is to understand molecular mechanisms of tooth regeneration and tissue renewal in vertebrates. Our research benefits from explicit integration of experimental systems (cichlid fishes, zebrafish, mouse) and a unique approach translating new biology from fishes to mammals and back. In this competitive renewal application, we build on the previous project period to outline three Specific Aims that fuse unbiased and experimental approaches in multiple vertebrate tooth models to identify cell populations and molecular signals that direct natural whole-tooth replacement (cichlids and zebrafish) and mouse incisor tissue renewal. The gaps we aim to fill are significant. One in five humans presents with a genetic disorder affecting the dentition and nearly 100% develop problems (e.g, cavities) with age. 30% of people worldwide over the age of 65 lack teeth entirely. Our proposed research will provide answers to the basic question of how regenerating teeth are partitioned into coordinated zones of renewal and differentiation, and how mammalian teeth recover from damage. Insights should promote innovative strategies for bio-inspired regenerative dentistry.

项目总结/摘要 这项研究的主要目标是了解牙齿再生的分子机制 以及脊椎动物的组织更新。我们的研究得益于实验系统的明确整合 （慈鲷鱼，斑马鱼，小鼠）和一种独特的方法将新的生物学从鱼类转化为哺乳动物 再回来在这个竞争性的续约申请中，我们在上一个项目期间概述了三个 在多个脊椎动物牙齿模型中融合无偏和实验方法的特定目的， 鉴定指导天然全牙替换的细胞群和分子信号（慈鲷和 斑马鱼）和小鼠切牙组织更新。我们要填补的空白是巨大的。五分之一的人类 出现影响牙列的遗传性疾病，几乎100%出现问题（例如蛀牙） 随年龄全世界65岁以上的人中有30%完全没有牙齿。我们的研究将提供 对再生牙齿如何被划分为协调的再生区域这一基本问题的回答 以及哺乳动物牙齿如何从损伤中恢复。洞察力应促进创新 生物启发再生牙科的策略。

项目成果

Common developmental pathways link tooth shape to regeneration.

• DOI: 10.1016/j.ydbio.2013.02.007
• 发表时间: 2013-05-15
• 期刊: DEVELOPMENTAL BIOLOGY
• 影响因子: 2.7
• 作者: Fraser, Gareth J.;Bloomquist, Ryan F.;Streelman, J. Todd
• 通讯作者: Streelman, J. Todd

The genetic basis of a complex functional system.

复杂功能系统的遗传基础。

• DOI: 10.1111/j.1558-5646.2012.01688.x
• 发表时间: 2012
• 期刊: Evolution; international journal of organic evolution
• 作者: Parnell,NicholasF;Hulsey,CDarrin;Streelman,JTodd
• 通讯作者: Streelman,JTodd

Protocol for comparing gene-level selection on coding mutations between two groups of samples with Coselens.

• DOI: 10.1016/j.xpro.2023.102117
• 发表时间: 2023-03-17
• 期刊: STAR protocols
• 作者: Iranzo J;Gruenhagen G;Calle-Espinosa J;Koonin EV
• 通讯作者: Koonin EV

Genome-enabled discovery of evolutionary divergence in brains and behavior.

• DOI: 10.1038/s41598-021-92385-8
• 发表时间: 2021-06-21
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Patil C;Sylvester JB;Abdilleh K;Norsworthy MW;Pottin K;Malinsky M;Bloomquist RF;Johnson ZV;McGrath PT;Streelman JT
• 通讯作者: Streelman JT

Developmental Plasticity of Patterned and Regenerating Oral Organs.

• DOI: 10.1016/bs.ctdb.2015.07.005
• 发表时间: 2015
• 期刊: Current topics in developmental biology
• 作者: Streelman JT;Bloomquist RF;Fowler TE
• 通讯作者: Fowler TE