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Detection and Characterization of Blood-borne Prions

血源性朊病毒的检测和表征

基本信息

批准号：
10201450
负责人：
Candace K. Mathiason
金额：
$ 52.53万
依托单位：
COLORADO STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-06-01 至 2025-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10201450
关键词：
Affect Agriculture American Amyloid Animals Asia Biological Biological Assay Blood Blood Cells Blood Platelets Blood Transfusion Blood specimen Canada Cell Fraction Centrifugation Chronic Wasting Disease Chronology Clinical Collaborations Collection Consumption Coupled Creutzfeldt-Jakob Syndrome DNA Sequence Alteration Deer Detection Disease Drosophila genus Environment Europe Excretory function Genetic Genetic Carriers Grant Health Hematogenous Human Industry Infection Kinetics Label Letters Leukocytes Methodology Methods Monitor Nature Oral Pathogenesis Patients Phenotype Population PrP Prevalence Prion Diseases Prions Reindeer Research Risk Safety Sampling Scrapie Seeds Sheep Sorting - Cell Movement Testing Therapeutic Tissues Transgenic Mice Translating Variant Zoonoses cervid cost effective health economics improved in vitro Assay in vivo inhibitor/antagonist magnetic beads mutation carrier pre-clinical prevent prion seeds prototype trafficking translational impact transmission process

项目摘要

No practical noninvasive antemortem test exists to detect cervid, human, or any, prion disease. Blood- borne transmission of prions from asymptomatic human variant Creutzfeldt Jakob Disease (vCJD)-infected humans has occurred(12,14-16). Chronic wasting disease (CWD) is an emergent rapidly spreading disease in cervids(27,28,37-40) with uncertain zoonotic potential(3-8). An estimated one in 36 Americans hunt deer and/or elk, resulting in an estimated 7,000-15,000 CWD-infected cervids consumed annually in the USA (increasing 20%/yr)(2). As hunting is a roughly $26 billion (per annum) industry(1), CWD represents an enormous potential health and economic threat. The commonalities among vCJD and cervid CWD, including the presence of hematogenous infectivity(34,47), are remarkable. Antemortem detection and surveillance of human, and animal, prion disease has been hindered by the combination of low prion concentrations and abundant assay inhibitors in blood and other accessible biological samples. We have developed simple, rapid, specific and highly sensitive in vitro assays to detect blood-borne prions(9-11). In this competing renewal we will quantitate the temporal distribution and specific blood cell phenotypes critical to prionemia in serially-collected human and cervid blood samples. The translational impact of the results of these studies will: a) improve human blood transfusion safety, b) provide a better means to assess and monitor developing prion therapeutics, c) largely eliminate the risks of human consumption of cervid prions, d) provide greater understanding of the prevalence and consequences of CWD to the cervid hunting and agriculture industries, and e) elucidate our understanding of prionemia in disease pathogenesis. Thereby, this research will have immediate impacts in both human and animal health.

目前还没有实用的无创死前检测方法来检测宫颈、人类或任何朊病毒疾病。血- - - - - -