Protein-Glycan Interaction Resource at the National Center for Functional Glycomics (NCFG)

摘要/摘要 该R24申请NIGMS国家和地区资源是为了使研究人员在以下方面- 糖科学服务领域，其研究需要糖科学专业知识，继续和 改善对最先进技术的获取，以促进生物医学研究和人类健康，包括 蛋白质-多聚糖相互作用和多聚糖识别。糖基化是最常见和最多样的后遗症 翻译修饰(PTM)在所有生物中，每个细胞和有机体产生独特的PTM和 还有糖脂。这里建议的这种资源既不适用于个别实验室，也不适用于这些实验室 商业上可用的特定技术。拥有约7,000名用户(83.6%的新用户)和数百名 分别利用NCFG数据库和资源与功能糖组相关的实验室，我们的 资源显然得到了很好的利用，代表着一种独特的资源，所有生物医学人员都可以民主地访问 研究人员。近10,000个独立的、不同的多糖微阵列证明了需求的增加 仅在过去4年内就在NCFG资源中心使用。我们对蛋白质-葡聚糖相互作用的强调是 及时，因为研究继续发现这些相互作用是理解 糖链在生物系统中的表达和功能及其被抗体、糖链结合的识别 人类和动物系统中的蛋白质(GBP)和凝集素，以及微生物病原体和肠道微生物组。 我们的资源提供了令人难以置信的各种糖链微阵列技术、糖链文库和 多个数据库在其他地方不可用。我们的资源的成功已导致100多个 用户在过去5年中发表的出版物。由于它的成功和迅速增加的请求数量， 由于我们是糖科学中唯一可用的这类资源，我们提出了三个具体的 旨在继续使这些宝贵的资源变得可访问。目标1-我们将继续产生和 改进已定义的糖、糖肽、糖蛋白和糖脂微阵列，以及基于Luminex的 糖珠技术，都是资源独一无二的。目标2-我们将提供并进一步改进 天然双功能荧光标记(BftS)最初由NCFG首创，将用于多糖 微阵列和微珠结合物。由NCFG首创的这种新颖的BftS为我们提供了先进的能力 标记复杂混合物中的葡聚糖，纯化它们，然后定量和定量打印微阵列 不同的密度。目标3-我们将继续使用BFT技术来生产和改进天然多糖 来自多种来源的细胞、组织和糖结合物的微阵列，构成了一种独特而有价值的工具 为资源中心服务。因此，我们的蛋白质-葡聚糖相互作用资源提供了独特的葡聚糖- 具有约束性的专业知识和技术、教学机会以及广泛的服务和对 新兴领域迅速被认为是健康和疾病的关键因素。

Abstract/Summary This R24 application for an NIGMS National and Regional Resource is to enable researchers in the under- served area of glycosciences and whose research needs glycoscience expertise, to have continued and improved access to state-of-the-art technologies to advance biomedical research and human health involving protein-glycan interactions and glycan recognition. Glycosylation is the most common and varied post- translational modification (PTM) in all living things, and each cell and organism generates unique PTMs and also glycolipids. Such resources proposed here are neither available to individual laboratories, nor are these specific technologies available commercially. With a base of ~7,000 users (83.6% new users) and hundreds of laboratories utilizing NCFG databases and resources, respectively, related to functional glycomics, our resource is obviously well used and represents a unique resource democratically accessible to all biomedical researchers. The increasing demand is evidenced by the nearly 10,000 individual, different glycan microarrays used at the NCFG resource center in just the last 4 years. Our emphasis on protein-glycan interactions is timely because research continues to uncover clues that these interactions are key to understanding the expression and functions of glycans in biological systems and their recognition by antibodies, glycan-binding proteins (GBPs) and lectins, in human and animal systems, and by microbial pathogens and gut microbiome. Our resource makes available an incredible variety of glycan microarray technologies, glycan libraries, and multiple databases not available elsewhere. The success of our resource has resulted in over a hundred publications by users in the past 5 years. Because of its success and rapidly increasing number of requests, and because we are the only resource of this kind available in the glycosciences, we propose three Specific Aims to continue making these invaluable resources accessible. Aim 1- We will continue to generate and improve defined glycan, glycopeptide, glycoprotein, and glycolipid microarrays, along with Luminex-based glycan-bead technologies, all unique to the resource. Aim 2- We will make available and further improve natural bifunctional fluorescent-tags (BFTs) originally pioneered by the NCFG, and will be utilized in glycan microarray and bead conjugations. Such novel BFTs, pioneered by the NCFG, give us advanced capabilities to label glycans in complex mixtures, purify them, and then quantify and quantitatively print microarrays at varying densities. Aim 3- We will continue to use BFT technology to produce and improve natural glycan microarrays from many sources of cells, tissues, and glycoconjugates, comprising a unique and valuable tool for the resource center. Thus, our Protein-Glycan Interaction Resource provides unique capabilities in glycan- binding expertise and technologies, teaching opportunities, and a wide range of services and outreach to an emerging field rapidly becoming recognized as a key factor in health and disease.