National Center for Functional Glycomics

描述（由申请人提供）： 在这里，我们建议建立一个“国家功能性糖组学中心”（NCFG），专注于5个主要目标：技术研究和开发（TR&D）;推动与TR&D项目相关的生物医学项目（DBP）;合作服务;培训;以及技术和研究发现的传播。该应用程序的开发是埃默里大学医学院糖组学中心非常成功的领导的产物，该中心拥有成功的功能糖组学联盟（CFG）的蛋白质-聚糖相互作用核心（Core H），这是2001-2011年由NIGMS资助的独特资源，已被全球数百名研究人员使用。该中心对CFG定义聚糖微阵列的应用在过去8年中收到了超过800份分析请求，彻底改变了蛋白质-聚糖相互作用的研究。确定的聚糖微阵列已经成为研究凝集素、抗体和病毒的聚糖结合特异性的基准，对于本基金申请，这些被称为聚糖结合蛋白（GBP）。固定化聚糖在研究GBP和功能性糖组学方面的价值得到了该中心与全球600多名主要研究者建立的合作的证明，在过去8年中，有超过350篇同行评议的出版物。在过去的几年里，埃默里大学糖组学中心的成员，谁是在这个拟议的NCFG的首席研究员，提供了创新的领导，在开发技术，研究细胞糖组的功能和糖组学分析。在为NCFG提出的3个研发项目中，我们将1）扩展聚糖微阵列技术，2）开发“Shotgun Glycomics”作为研究天然细胞衍生聚糖识别的通用方法，3）开发不同的聚糖展示技术，以及细胞化身-以独特呈现方式代表细胞聚糖的颗粒。DBP包括对聚糖在微生物和病毒感染中的作用，聚糖和GBP在细胞粘附，先天性和适应性免疫反应中的作用，以及有助于GBP相互作用的糖萼内的识别因子的研究。NCFG将利用这些进展来推动一个强大的协作服务组件，以帮助生物医学研究界使用和开发聚糖微阵列和其他方法来探索聚糖识别。我们将提供一个强大的培训计划，以及启动多个场地和战略，以传播NCFG的技术和科学发展。

DESCRIPTION (provided by applicant): Here we propose to establish a "National Center for Functional Glycomics" (NCFG) that is focused on 5 major objectives; Technology Research and Development (TR&D); Driving Biomedical Projects (DBPs) tied to TR&D Projects; Collaborative Services; Training; and Dissemination of Technologies and Research Discoveries. The development of this application is an outgrowth of the very successful leadership provided by the Glycomics Center at Emory University School of Medicine, which housed the Protein-Glycan Interaction Core (Core H) of the successful Consortium for Functional Glycomics (CFG), a unique NIGMS-funded resource from 2001-2011 that has been utilized by hundreds of investigators worldwide. The Center's applications of the CFG's Defined Glycan Microarray, which has received >800 requests for analyses in the past 8 years, has revolutionized studies on protein-glycan interactions. Defined glycan microarrays have become the benchmark in studying the glycan-binding specificity of lectins, antibodies, and viruses, which for this grant application are termed glycan-binding proteins (GBPs). The value of immobilized glycans in studying GBPs and functional glycomics is evidenced by the collaborations already established in the Center with over 600 Principal Investigators worldwide, resulting in >350 peer-reviewed publications in the past 8 years. Over the past several years, the members of the Glycomics Center at Emory, who are the lead investigators in this proposed NCFG, have provided innovative leadership in developing technologies for studying the functions of cellular glycomes and for glycomic analyses. In the 3 TR&D Projects proposed for this NCFG, we will 1) expand glycan microarray technologies, 2) develop "Shotgun Glycomics" as a general method for studying natural cell-derived glycan recognition, and 3) develop different glycan display technologies, as well as cellular avatars - particles that represent cellular glycomes in unique presentations. The DBPs include studies on the roles of glycans in microbial and viral infections, glycans and GBPs in cell adhesion, innate and adaptive immune responses, and recognition factors within the glycocalyx that contribute to GBP interactions. The NCFG will use these advances to drive a strong Collaborative Service component to aid the biomedical research community in the using and developing of glycan microarrays and other approaches to explore glycan recognition. We will offer a robust Training program, as well as initiate multiple venues and strategies to Disseminate the technological and scientific developments in the NCFG.