Core C - Bone Core

核心C：骨核摘要 该计划的骨核项目将为采购提供共享的设施和服务， 用于临床标本和动物模型的组织的处理和解释 该计划的所有项目。这将通过以下目标实现： 目的1.实验设计/咨询。这包括有关端点的方法 分析和方法的能力和局限性。 目的2.病理解释。对于组织标本，组织病理学和 免疫组织化学评分。 目的3.组织的加工。骨组织和软组织资源的汇集 组织处理。这包括固定、脱钙、石蜡包埋和切片 脱钙切片和固定、塑料包埋和未脱钙切片。 目的4.矿化组织常规染色和特殊染色。这包括用于 矿化组织、破骨细胞和免疫组织化学。 目的5.骨切片和肿瘤组织的组织形态计量学分析。 这包括测量特定的与骨骼相关的结构和细胞参数。 目的6.临床组织标本的采购。骨髓样本用于 根据需要分离播散性肿瘤细胞和血液以供循环肿瘤细胞和血清。 这些服务的核心支持将提高样本分析的效率并促进 通过相似的模型系统及其共同分析实现项目之间的相互作用。一个 作为一个整体，该计划的重大好处将是分析以下各项的标准化格式 来自常见动物模型的标本将使用不同的实验 接近/目标。这将提供可共享和比较的有价值的信息 该计划中的所有项目。为了实现这些目标，核心是由Dr。 骨骼生物学专家肯尼斯·科兹洛夫说。此外，核心还得到了Dr。 罗希特·梅赫拉，委员会认证的泌尿外科肿瘤学病理学家和托德·摩根博士，泌尿科医生 和内科科学家，在播散性肿瘤细胞获取和生物学方面具有专业知识。 骨组织形态计量学方面的其他专业知识由我们的顾问Tom Wronski博士a提供 大卫·卢卡斯博士在这一领域和骨病理方面的国家公认专家，解剖学家 专门研究骨骼的病理学家。总体而言，该核心将继续提供全面的 以及本计划所有项目所需的独特服务。

CORE C: Bone Core Abstract The Bone Core of the Program Project will provide shared facilities and services for procuring, processing and interpretation of tissues from both clinical samples and animal models utilized in all projects of the program. This will be achieved through the following aims: Aim 1. Experimental design/consultation. This include methods regarding endpoint analyses and abilities and limitations of methods. Aim 2. Pathological interpretation. For histologic specimens, histopathology and scoring of immunohistochemistry. Aim 3. Processing of tissues. Both osseous tissues and pooling resources for soft tissue processing. This includes fixation, decalcification, paraffin embedding and sectioning of decalcified sections and fixation, plastic embedding and sectioning of undecalcified sections. Aim 4. Routine and special stains for mineralized tissues. This includes for mineralized tissues, osteoclasts and immunohistochemistry. Aim 5. Histomorphometric analysis of both osseous sections and tumor tissue. This includes measurement of specific bone-related structural and cellular parameters. Aim 6. Procurement of clinical tissue samples. Bone marrow samples for disseminated tumor cells isolation and blood for circulating tumor cells and serum as needed. Core support of these services will promote efficiency of specimen analysis and facilitate interactions between projects through the similar model systems and their common analyses. A significant benefit to the program as a group will be a standardized format for analysis of specimens from the common animal models that will be using different experimental approaches/targets. This will provide valuable information that can be shared and compared across the projects in the program. To accomplish these goals, the core is directed by Dr. Kenneth Kozloff, who is an expert in bone biology. In addition, the core is supported by Dr. Rohit Mehra, a board-certified urologic oncology pathologist and Dr. Todd Morgan, a urologist and physician scientist with expertise in disseminated tumor cell procurement and biology. Additional expertise in bone histomorphometry is provided by our consultant Dr. Tom Wronski a nationally recognized expert in this field and in bone pathology by Dr. David Lucas, an anatomic pathologist who specializes in bone. Overall this core will continue to provide comprehensive and unique services that are required by all the projects of this Program.