Virus & Reservoirs Core

核心D：病毒和宿主 摘要 病毒和水库核心(核心D)的任务是提供无与伦比的分子病毒学和 在校园内为宾夕法尼亚大学CFAR调查人员提供排序支持、服务、设施、咨询和培训， 以及更广泛的艾滋病毒/艾滋病研究界。通过这项尖端的使能技术 与积极主动的科学领导相结合，Core D寻求在高度优先的科学领域推动艾滋病研究 方向。在本报告所述期间，核心D进行了重大修改，以满足不断变化的需求 需求、机会和优先事项，并领导艾滋病毒治疗领域的前瞻性研究议程， 对持续病毒库的发病机制的认识。这些变化还包括新的服务 随着由凯瑟琳·巴尔博士(主任)和穆罕默德·阿卜杜勒博士组成的新领导团队的成立- Mohsen(联席主任)和三名资深艾滋病毒/艾滋病科学家作为核心调查人员(Frederic Bushman博士， Beatrice Hahn和George Shaw)，为领导团队提供专业服务和指导。 为支持其使命，病毒和水库核心提供：(1)最先进的分子病毒学服务 包括广泛的病毒库、规模经济的病毒检测服务、创新的细胞产品 逆转录病毒载体转导体外扩增细胞的释放试验以支持创新的基因和细胞治疗 临床试验，定量实时和数字滴状聚合酶链式反应平台；(2)最先进的测序 提供分析支持服务，包括单基因组扩增、Illumina扩增片段测序和 具有定制处理和分析支持的Sanger平台，以及针对复杂种群的深度测序 或整合部位分析；(3)基于新型传播者/创建者SHIV(SIV/HIV嵌合体)的新服务 病毒，为了利用新一代SHIV的力量，拥有巨大的疫苗机会， 致病机理和治愈研究；(4)用于高敏感性和专门化病毒分析的新平台，包括 病毒转录本的量化、前病毒整合、单拷贝病毒载量、单细胞分析及相关 技术；(5)指导和教育适当应用这些新兴技术以及 实施方面的培训；和(6)积极主动的宣传和领导，以促进利用这些资源的研究 技术，包括与艾滋病毒储存库和组织免疫学SWG的密切合作。 在本报告所述期间，核心D向119名调查员提供了支助，#年创收500 000 000美元。 按存储容量使用计费，并支持140多个由NIH资助的应用程序。展望即将到来的周期， 与临床、免疫学和非人灵长类核心以及储存库和组织密切合作 免疫学SWG，核心D将扩大其服务，以激励基础和翻译实验室研究，预 在非人类灵长类动物中的临床研究，以及第一次人类临床试验，研究艾滋病毒宿主，病毒持久性， 和治愈策略。

Core D: Virus and Reservoirs ABSTRACT The mission of the Virus and Reservoirs Core (Core D) is to provide unparalleled molecular virology and sequencing support, services, facilities, consultation, and training to Penn CFAR investigators on campus, as well as the HIV/AIDS research community more broadly. Through this cutting-edge enabling technology combined with proactive scientific leadership, Core D seeks to advance AIDS research in high priority scientific directions. In the current reporting period, Core D has made substantial changes to both meet evolving needs, opportunities and priorities, and lead a forward-looking research agenda in the area of HIV cure, pathogenesis understanding of persistent viral reservoirs. These changes include new services as well as installation of a new leadership team comprised of Drs. Katharine Bar (Director) and Mohamed Abdel- Mohsen (Co-Director), and three senior HIV/AIDS scientists as Core Investigators (Drs. Frederic Bushman, Beatrice Hahn and George Shaw) to provide both specialized services and mentorship to the leadership team. To support its mission, the Virus and Reservoirs Core provides: (1) State-of-the-art molecular virology services including an extensive virus repository, viral assay services for economy of scale, an innovative cell product release assay for retroviral vector transduced ex vivo expanded cells to support innovative gene- and cell-therapy clinical trials, and quantitative real-time and digital droplet PCR platforms; (2) State-of-the-art sequencing services with analytical support, including Single Genome Amplification, amplicon sequencing by Illumina and Sanger platforms with custom processing and analytic support, and deep sequencing for complex populations or integration site analysis; (3) A new service based on novel transmitted/founder SHIV (SIV/HIV chimeric) viruses, to leverage the power of a new generation of SHIVs with tremendous opportunity for vaccine, pathogenesis and cure research; (4) Novel platforms for highly sensitive and specialized viral analysis including quantification of viral transcripts, proviral integration, single-copy viral load, single-cell analysis and related techniques; (5) Mentoring and education in appropriate application of these emerging technologies as well as training in implementation; and (6) Proactive outreach and leadership to promote research that exploits these technologies, including close collaboration with the HIV Reservoirs & Tissue Immunology SWG. In the current reporting period, Core D provided support to 119 investigators, generated over $5,000,000 in chargebacks, and supported more than 140 NIH-funded applications. Looking ahead to the upcoming cycle, in close collaboration with the Clinical, Immunology and Nonhuman Primate Cores and the Reservoirs and Tissue Immunology SWG, Core D will expand its services to galvanize basic and translational laboratory research, pre- clinical studies in nonhuman primates, and first-in-human clinical trials studying HIV reservoirs, virus persistence, and cure strategies.