Advanced glycation endproducts (AGEs) as metabolic by-products that mediate neurodegeneration.

晚期糖基化终产物 (AGE) 作为介导神经退行性变的代谢副产物。

• 批准号: 10213648
• 负责人: Pankaj Kapahi
• 金额: $ 64.69万
• 依托单位: BUCK INSTITUTE FOR RESEARCH ON AGING
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-15 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10213648
• 关键词: Adult; Advanced Glycosylation End Products; Age; Aging; Alzheimer' s Disease; Alzheimer' s disease model; Alzheimer' s disease risk; Amyloid beta-Protein; Arginine; Binding Proteins; Biochemical; Biochemical Pathway; Caenorhabditis elegans; Cellular Stress; Cerebrospinal Fluid; Chemicals; Chronic; Complement; Complex; DNA; Data; Defect; Diabetes Mellitus; Diet; Disease; Drug Metabolic Detoxication; Exhibits; Fatty Acids; Genes; Genetic; Glucose; Glutathione; Glycolysis; Goals; Human; Hyperglycemia; Hypersensitivity; Ketone Bodies; Lactoylglutathione Lyase; Link; Lipids; Longevity; Mediating; Mediator of activation protein; Metabolic; Metabolic Pathway; Metabolic dysfunction; Metabolism; Modeling; Nerve Degeneration; Neurodegenerative Disorders; Neurons; Obesity; Paralysed; Pathogenesis; Pathology; Pathway interactions; Pharmacology; Play; Post-Translational Protein Processing; Production; Proteins; Pseudouridine; Pyruvaldehyde; RNA Splicing; Reaction; Risk; Role; Series; Serum; Testing; Touch sensation; Toxic effect; Work; age effect; age related; age related neurodegeneration; anaerobic glycolysis; base; cell injury; crosslink; diabetic; fatty acid oxidation; feeding; glucose metabolism; lipid metabolism; mortality; mutant; neurotoxicity; new therapeutic target; normal aging; overexpression; protein aggregation; proteotoxicity; response; tau Proteins; tau mutation; therapeutic target

PROJECT SUMMARY/ ABSTRACT Aging and chronic hyperglycemia results in several metabolic and biochemical perturbations including elevation of a series of highly reactive α-dicarbonyl compounds (α-DCs, e.g., Methylglyoxal(MGO). α-DCs are unavoidable byproducts largely of anaerobic glycolysis which react indiscriminately with proteins, lipids, and DNA to yield a heterogeneous group of molecules called advanced glycation end products (AGEs). A large body of evidence has linked accelerated glucose metabolism and diabetes to neurodegenerative diseases like Alzheimer's disease (AD). We hypothesize that toxic byproducts of glucose metabolism that result in the formation of AGEs explain the enhanced risk of AD due to hyperglycemia and diabetes. In support of this AGEs in serum and AGE crosslinking in protein aggregates have been associated with enhanced neurodegeneration in AD. However, AGEs are hard to model as they take years to accumulate in humans and the mechanism by which they cause cellular damage remains to be elucidated. To overcome this gap, we have established C. elegans (worm) models that significantly accumulate α-DCs and AGEs, exhibiting several age- related pathologies, such as hypersensitivity to touch, neuronal damage, paralysis, and early mortality, all within three weeks of adulthood. In addition, we have observed that direct administration of synthetic methylglyoxal derived AGEs can directly cause neurotoxicity. Furthermore, we have observed that a C. elegans model overexpressing the pro-aggregating form of tau, that has been implicated in Alzheimer's disease, is sensitive to feeding either glucose or AGEs in the diet. In this proposal, we will test the hypothesis that changes in glucose and lipid metabolism pathways, especially with age, influence MGO and associated AGEs thereby causing neurodegeneration associated with AD. We will also determine the mechanisms by which AGEs influence metabolic dysfunction and contribute to neurodegeneration in AD. In Aim 1 we will explore a causal role for the effects of AGEs on neurodegeneration in normal aging and in Alzheimer's disease models using synthetically derived AGEs. We will also examine the role of age-associated changes in glucose metabolism in influencing the levels of MGO and AGEs and enhancing neurodegeneration in models of AD. In Aim 2 we will determine the relationship between lipid metabolism and production of AGEs. We will genetically and pharmacologically manipulate fatty acid oxidation pathways to examine their influence on modulating neurodegeneration in normal aging and AD models through modulation of AGEs. In Aim 3 we propose to identify the mechanisms by which AGEs mediate their toxicity leading to inhibition of fatty acid oxidation and neurodegeneration. We will identify AGE-binding proteins and therapeutic targets to modulate AGE-related neurodegeneration. These studies will identify several genetic and pharmacological targets to ameliorate AGEs and slow down the progression of neurodegeneration in AD.

项目摘要/摘要