Core C: Genetics and Genomics

生物医学核心C(遗传学与基因组学)：综述 加州大学旧金山分校-NORC的58个实验室中有24个在他们的 研究。一些人的重点是发现调节摄食行为、新陈代谢或营养的基因变化 与人类肥胖及其健康并发症相关的动态平衡。其他人则使用基因定义的 动物模型来研究这些过程背后的机制。体内基因的靶向改变 这些模型生物是一种简单的方法来检验关于这些生物参与的分子假说 小路。还有一些人使用来自人类、模型生物或细胞系的样本的表达谱来 建立参与能量平衡的候选路径。 加州大学旧金山分校NORC遗传学和基因组学核心提供使用、协助和培训 一系列用于分子遗传和基因组评估的复杂方法和仪器 营养和肥胖的潜在途径。由于遗传学和基因组学核心设施由五个人监督 加州大学旧金山分校的行政单位，这个核心提供了NORC用户使用的基础设施 那些设施。遗传学和基因组学核心帮助NORC用户识别和访问这些设施。 NORC还支持开发新的遗传和基因组技术，这些技术是通过 对于NORC研究基地来说，一个明确定义的过程是新出现的需要。 NORC遗传学和基因组学核心提供了以下工具和设施： 1.全球表达分析，包括单细胞测序，以揭示相关的途径 实验室干预研究。 2.涉及模式生物或细胞系基因改变的功能研究，包括有针对性的 基因敲除/插入和转基因。 3.维护和生成NORC研究所需的动物模型。 总体而言，这一核心降低了方法障碍，帮助NORC研究人员实现高效和适当的 遗传学和基因组学工具的应用。这些能力加速了各种不同和相互关联的 研究肥胖、营养、食物摄入和新陈代谢。

Biomedical Core C (Genetics & Genomics): Summary Twenty-four of the 58 laboratories within the UCSF-NORC employ genetics or genetic manipulation in their research. Some focus on uncovering genetic alterations regulating feeding behavior, metabolism or nutritional homeostasis associated with obesity and its health complications in humans. Others use genetically-defined animal models to study the mechanisms underlying those processes. The targeted alteration of genes within those model organisms is a tractable method to test molecular hypotheses about the involvement of those pathways. Still others use expression profiling of samples from humans, model organisms or cell lines to establish candidate pathways involved in energy balance. The UCSF NORC Genetics and Genomics Core provides access to, assistance with, and training in the use of an array of sophisticated methods and instruments for genetic and genomic assessment of molecular pathways underlying nutrition and obesity. As the Genetics and Genomics Core facilities are overseen by five administrative units at UCSF, this Core provides the infrastructure through which NORC users make use of those facilities. The Genetics and Genomics Core assists NORC users to identify and access those facilities. The NORC also supports the development of novel genetic and genomic technologies that are determined via a well-defined process to be of emerging need to the NORC research base. The NORC Genetics and Genomics Core provides tools and facilities for: 1. Global expression analyses, including single cell sequencing, to uncover relevant pathways in laboratory interventional studies. 2. Functional studies involving genetic alteration of model organisms or cell lines, including targeted knock-outs/ins and transgenics. 3. Maintaining and generating animal models required for NORC research. Overall, this Core lowers methodological barriers to help NORC researchers achieve the efficient and proper application of genetics and genomics tools. These capabilities accelerate a variety of diverse and interrelated studies of obesity, nutrition, food intake and metabolism.