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Core C: Genetics and Genomics

核心 C：遗传学和基因组学

基本信息

批准号：
10457902
负责人：
Jeremy F Reiter
金额：
$ 19.02万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-08-01 至 2025-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10457902
关键词：
Affect Animal Model Area Bioinformatics Cell Line Cells Chimera organism Chromosomes Core Facility DNA Development Eating Embryo Transfer Equipment Evolution Expression Profiling Feeding behaviors Gene Mutation Gene-Modified Generations Genes Genetic Genomics Genotype Goals Health Homeostasis Human Individual Infrastructure Injections Intervention Studies Knock-out Laboratories Mediating Metabolism Methodology Methods Microinjections Modification Molecular Mouse Strains Mutation Nutritional Obesity Pathway interactions Physiology Ploidies Procedures Process Production Research Research Personnel Resources Sampling Services Specialist Technical Expertise Technology Testing Tissues Training Transgenic Mice Transgenic Organisms base blastocyst cost cost effective embryonic stem cell energy balance exome gene function genetic analysis genetic manipulation genomic tools innovation instrument member next generation sequencing novel nutrition programs repository single cell sequencing tool transcriptome

项目摘要

Biomedical Core C (Genetics & Genomics): Summary Twenty-four of the 58 laboratories within the UCSF-NORC employ genetics or genetic manipulation in their research. Some focus on uncovering genetic alterations regulating feeding behavior, metabolism or nutritional homeostasis associated with obesity and its health complications in humans. Others use genetically-defined animal models to study the mechanisms underlying those processes. The targeted alteration of genes within those model organisms is a tractable method to test molecular hypotheses about the involvement of those pathways. Still others use expression profiling of samples from humans, model organisms or cell lines to establish candidate pathways involved in energy balance. The UCSF NORC Genetics and Genomics Core provides access to, assistance with, and training in the use of an array of sophisticated methods and instruments for genetic and genomic assessment of molecular pathways underlying nutrition and obesity. As the Genetics and Genomics Core facilities are overseen by five administrative units at UCSF, this Core provides the infrastructure through which NORC users make use of those facilities. The Genetics and Genomics Core assists NORC users to identify and access those facilities. The NORC also supports the development of novel genetic and genomic technologies that are determined via a well-defined process to be of emerging need to the NORC research base. The NORC Genetics and Genomics Core provides tools and facilities for: 1. Global expression analyses, including single cell sequencing, to uncover relevant pathways in laboratory interventional studies. 2. Functional studies involving genetic alteration of model organisms or cell lines, including targeted knock-outs/ins and transgenics. 3. Maintaining and generating animal models required for NORC research. Overall, this Core lowers methodological barriers to help NORC researchers achieve the efficient and proper application of genetics and genomics tools. These capabilities accelerate a variety of diverse and interrelated studies of obesity, nutrition, food intake and metabolism.

生物医学核心C（遗传学和基因组学）：摘要 UCSF-NORC内的58个实验室中有24个采用遗传学或遗传操作， research.有些研究侧重于揭示调节摄食行为、代谢或营养的遗传改变。与人类肥胖及其健康并发症相关的内稳态。其他人使用基因定义的动物模型来研究这些过程的机制。有针对性地改变体内的基因这些模式生物是一个易于处理的方法来测试分子假说的参与，途径。还有一些人使用来自人类、模式生物或细胞系的样品的表达谱，建立参与能量平衡的候选途径。 UCSF NORC遗传学和基因组学核心提供使用，援助和培训，一系列复杂的方法和仪器，用于分子遗传和基因组评估，营养和肥胖的潜在途径。由于遗传学和基因组学核心设施由五个 UCSF的管理单位，该核心提供了NORC用户使用的基础设施，这些设施。遗传学和基因组学核心帮助NORC用户识别和访问这些设施。 NORC还支持开发新的遗传和基因组技术，这些技术是通过一个定义明确的过程是NORC研究基地的新兴需求。 NORC Genetics and Genomics Core提供以下工具和设施： 1.全球表达分析，包括单细胞测序，以揭示相关的途径，实验室干预研究。 2.涉及模式生物或细胞系遗传改变的功能研究，包括靶向敲除/插入和转基因。 3.维护和生成NORC研究所需的动物模型。总体而言，该核心降低了方法障碍，以帮助NORC研究人员实现有效和适当的遗传学和基因组学工具的应用。这些能力加速了各种不同的和相互关联的肥胖、营养、食物摄入和新陈代谢的研究。