DNA Core

DNA合成资源核心

DNA Synthesis Resource Core The DNA Synthesis Resource Core plays an essential role in the successful integration of this Program Project by providing high purity, site-specifically modified oligonucleotides to Projects 1, 2 and 3. The DNA Synthesis Resource Core works closely with Project 1, where novel reagents and synthetic strategies are developed for the site-specific synthesis of oligonucleotides containing the Fapy-dG lesions of interest to the Program Project. The technology is transferred to the Core, where the desired modified oligonucleotides are synthesized on the appropriate scale and purity for further chemical (Project 1) and biochemical (Projects 1 and 2) studies, cell-based studies (Project 2), and structural and conformational analyses (Project 3). The Specific Aims for the DNA Synthesis Resource Core are to: 1) synthesize site-specifically modified oligonucleotides of interest to this PPG; 2) develop protocols for the purification of the modified oligonucleotides; and 3) provide initial characterization of modified oligonucleotide.