Core 3. Immunology and Pathology.

核心 3. 免疫学和病理学。

• 批准号: 10223113
• 负责人: Amy Rasley
• 金额: $ 45.95万
• 依托单位: UNIVERSITY OF CALIF-LAWRNC LVRMR NAT LAB
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-08 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10223113
• 关键词: Accreditation; Amber; Animals; Antigens; Atopobium vaginae; Back; Biological; Biological Assay; California; Cells; Chlamydia trachomatis; Collaborations; Communication; Data; Equipment; Fertilization; Formulation; Genital; Genitalia; Goals; Human; Human Resources; Immune response; Immunity; Immunologic Markers; Immunologics; Immunology; Immunology procedure; Individual; Infection; Infection prevention; Information Management; Laboratories; Lead; Location; Mediating; Medicine; Mucous Membrane; Mus; Nucleic Acid Vaccines; Operative Surgical Procedures; Pathogenesis; Pathologic; Pathologist; Pathology; Peripheral Blood Mononuclear Cell; Preparation; Procedures; Reporting; Research; Research Project Grants; Role; Safety; Sampling; Serum; Services; Shipping; Splenocyte; Survivors; Time; Training; Universities; Vaccinated; Vaccination; Vaccines; Vagina; Work; acute toxicity; animal facility; cell mediated immune response; cohort; efficacy study; experimental study; immunogenic; immunogenicity; immunopathology; in vivo; in vivo evaluation; instrumentation; laboratory facility; mouse model; nano; novel; research and development; response; scaffold; vaccine candidate; vaccine development; vaccine evaluation

SUMMARY/ABSTRACT CORE 3: IMMUNOLOGY AND PATHOLOGY Dr. Rasley (PI) and Dr. Weilhammer (Co-I) will establish and operate an Immunology and Pathology Core that serves the Cooperative Research Center for Nano-Scaffold Vaccines Against Chlamydia trachomatis (Ct) by providing facilities, expertise, effort, materials, and information management that helps achieve the aims of all three research projects. We have the established laboratory facilities, instrumentation, and storage necessary for all immunological assays that will be performed as part of this Core, as well as access to a state-of-the-art animal AAALAC accredited animal facility. We will establish and maintain standard operating procedures and training materials sufficient to develop the personnel with the expertise necessary to reproducibly perform procedures needed to support the immunological analyses needed by the individual projects. The Immunology and Pathology Core will be staffed to provide the effort required for all animal studies and subsequent immunologic analyses as well as properly acquiring and processing samples that will be sent offsite for analyses (pathology; UC Davis). The core will be highly centralized with personnel and facilities at Lawrence Livermore National Laboratory (LLNL), with the exception of the pathology analyses which will be performed at UC Davis. The Immunology and Pathology Core can also work as a user facility, providing training and access to facilities that might enable research and development for the individual projects that do not have the expertise or equipment at their locations. The core will also compile all data generated for each Project and provide a comprehensive report to the Project PIs. Using a platform such as Box, we envision data from the Cores be available to all team ambers at any given time in order to facilitate cross fertilization of ideas.

摘要/摘要 核心3：免疫学和病理学 拉斯利博士(PI)和韦尔哈默博士(Co-I)将建立和运营一个免疫和病理核心， 为沙眼衣原体(CT)纳米支架疫苗合作研究中心提供服务 提供设施、专业知识、努力、材料和信息管理，以帮助实现所有 三个研究项目。我们拥有必要的实验室设施、仪器和存储设备。 所有将作为该核心的一部分执行的免疫学分析，以及访问最先进的 AAALAC认证的动物设施。我们将建立和维护标准的操作程序和 培训材料足以培养具有可重复执行任务所需专业知识的人员 支持个别项目所需的免疫学分析所需的程序。免疫学 病理中心的工作人员将为所有动物研究和后续工作提供所需的工作 免疫学分析以及适当获取和处理将被送往异地进行分析的样本 (病理学；加州大学戴维斯分校)。核心将高度集中在劳伦斯·利弗莫尔的人员和设施上 国家实验室(LLNL)，但病理分析将在加州大学进行 戴维斯。免疫和病理核心也可以作为用户设施工作，提供培训和访问 可能为不具备专业知识的单个项目进行研究和开发的设施 或其所在位置的设备。核心还将汇编为每个项目生成的所有数据，并提供 向项目绩效指标提交全面报告。使用Box这样的平台，我们可以预见来自内核的数据 随时提供给所有团队琥珀，以促进思想的交叉培养。