Characterization of 3D kinematics and EMG and related information in neural activities recorded from the orofacial motor cortex during feeding in rats - Revision - 1

大鼠进食期间口面部运动皮层记录的神经活动的 3D 运动学和 EMG 特征及相关信息 - 修订版 - 1

• 批准号: 10227568
• 负责人: Kazutaka Takahashi
• 金额: $ 6.3万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-04 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10227568
• 关键词: 3-Dimensional; Behavior; Brain region; Central Nervous System Diseases; Chronic; Collaborations; Data; Deglutition; Dimensions; Electrodes; Electromyography; Fluoroscopy; Generations; Health; Impairment; Implant; Injury; Jaw; Joints; Knowledge; Laboratories; Life; Limb structure; Mastication; Methods; Microelectrodes; Motor Cortex; Movement; Muscle; Operative Surgical Procedures; Oral; Output; Parents; Pathologic; Patients; Physicians; Prevention; Process; Property; Quality of life; Rattus; Rehabilitation therapy; Research; Research Activity; Rodent; Science; Site; Speed; Techniques; Testing; Tongue; Training; Underrepresented Minority; awake; base; central nervous system injury; craniofacial structure; digital; experience; feeding; improved; kinematics; medical schools; member; motor control; motor impairment; neuroimaging; neurological rehabilitation; neuromechanism; novel; orofacial; relating to nervous system; skills; vocal cord

Project Summary (from parent R03) Impaired chewing and swallowing as a result of orofacial or CNS injury or disease is a worldwide health problem that can impact quality of life and even be life-threatening. Current rehabilitation of such impairments has largely overlooked recent advances in neurorehabilitation of limb motor control, which may explain why many patients cannot regain normal chewing and swallowing. The oral primary motor cortex (oM1) is the main brain region involved in the generation and control of orofacial movements. However, detailed baseline 3D kinematics and electromyography (EMG) activity of aerodigestive and craniofacial structure as a whole which is to be compared to the pathological cases is lacking. Detailed characterization of modulations of local field potentials (LFPs) to gape types remains unclear. Furthermore, detailed kinematic and EMG encoding in single unit spiking activities for any orofacial behavior has not been performed. Lastly, relation between LFPs and muscle activities has not been explored except for beta oscillation and tongue muscles. Thus, to fill this knowledge gap, our specific aims are: AIM 1: To characterize and quantify 3D kinematics of aerodigestive and craniofacial structures and jaw and tongue EMG activities during natural feeding in awake rats. We will utilize our documented expertise with 3D high-speed videofluoroscopy and chronically implanted jaw/tongue EMG electrodes. We will: (a) characterize gape cycle types (e.g., chewing, swallowing) during feeding and how epiglottal and vocal fold open/closure are timed at each of the cycle types; and (b) perform dimension reduction techniques on both kinematics and EMGs to obtain a set of principal movements and EMG activities for each cycle type and transitions between cycle type. AIM 2: To relate the jaw/tongue EMG and 3D kinematics of aerodigestive and craniofacial structures to simultaneously record neural activities within multiple oM1 sites and layers in awake rats, and test if and how oM1 neural activity properties are related to tongue and jaw EMG and 3D kinematics of aerodigestive and craniofacial structures during feeding. We will utilize our documented expertise with chronically implanted microelectrode arrays that span horizontally and vertically into oM1 layers 2/3 (mainly cortico-cortico projections) and 5/6 (mainly output projections). We will then: (a) characterize how LFP profiles are related to types of gape cycles and their transitions between them; (b) characterize how kinematic- and EMG activities are encoded in single unit spiking activity of oM1; and (c) characterize cortico-muscular coherence between oM1 LFPs and jaw/tongue EMG activities for each gape type. This proposal will define a new 3D kinematic characterization of aerodigestive and craniofacial structures during feeding and novel oM1 neural mechanisms in terms of modulations of LFPs to gape cycles and single unit spiking activity encoding of kinematics and EMG based on layers. Better understanding of such mechanisms is needed to develop improved prevention and management of impaired motor functions resulting from oral injury, possibly by targeting oM1 neural processes.

项目摘要(来自上级R03) 由于口腔面部或中枢神经系统损伤或疾病导致的咀嚼和吞咽障碍是一个世界性的健康问题。 会影响生活质量，甚至危及生命的问题。目前对这种损伤的康复 在很大程度上忽视了肢体运动控制神经康复方面的最新进展，这可能解释了为什么 许多患者无法恢复正常的咀嚼和吞咽。口腔初级运动皮质(OM1)是主要的 参与口面部运动的产生和控制的大脑区域。然而，详细的基线3D 空气消化结构和颅面结构整体的运动学和肌电活动 是要与病理病例相比较缺乏的。局域场调制的详细表征 张口型的潜伏期(LFP)仍不清楚。此外，详细的运动学和肌电编码在单个 任何口腔面部行为的单位尖峰活动都没有进行过。最后，LFP和LFP之间的关系 除了β振荡和舌肌之外，还没有研究过肌肉活动。因此，要填补这一点 知识鸿沟，我们的具体目标是：目标1：表征和量化空气消化的三维运动学 清醒大鼠自然喂养过程中的头面部结构、颌骨和舌肌电信号活动。我们 将利用我们记录在案的3D高速视频透视专业知识并长期植入 颌部/舌部肌电电极。我们将：(A)确定张口周期的类型(例如，咀嚼、吞咽) 喂养以及会厌和声带在每种周期类型下的打开/关闭时间；以及(B)执行 运动学和肌电的降维技术，以获得一组主要动作和 每种周期类型的肌电活动以及周期类型之间的转换。目的2：将颌骨/舌头肌电联系起来 以及空气消化和颅面结构的3D运动学，以同时记录神经 清醒大鼠多个OM1部位和层内的活动，并测试OM1神经活动是否和如何 舌颌肌电信号和空气、消化、颅面三维运动学特征相关 在进食过程中的结构。我们将利用我们记录在案的专业知识进行长期植入的微电极 水平和垂直跨越到OM1层2/3(主要是皮质-皮质投影)和5/6的阵列 (主要是产出预测)。然后，我们将：(A)描述LFP曲线与GAPE周期类型之间的关系 以及它们之间的转换；(B)表征运动学和肌电活动如何以单一的 OM1的单位尖峰活动；以及(C)表征OM1 LFP和OM1 LFP之间的皮质-肌肉一致性 每种张口类型的下颌/舌头肌电活动。该提案将定义一种新的3D运动学特征 进食过程中的空气消化和颅面结构以及新的OM1神经机制 LFP对张口周期的调制及运动学和肌电的单单位峰活动编码 层次感。需要更好地了解这种机制，以改进预防和管理 口腔损伤导致的运动功能受损，可能是通过靶向OM1神经突起。