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Studying Dynamics of Monocyte HIV RNA Persistence and the Interface with CNS Outcomes during Acute HIV Infection

研究急性 HIV 感染期间单核细胞 HIV RNA 持久性的动态及其与 CNS 结果的相互作用

基本信息

批准号：
10231240
负责人：
ELIZABETH IRENE LAWS
金额：
$ 3万
依托单位：
UNIVERSITY OF HAWAII AT MANOA
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-09-01 至 2022-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10231240
关键词：
Acute Address Agreement Anti-Inflammatory Agents Biological Assay Blood Brain CCL2 gene Cells Cerebrospinal Fluid Choline Creatine DNA Data Development Early Diagnosis Evaluation Event Extramural Activities Functional disorder Genetic Transcription Goals HIV HIV Infections Hawaii Immune System Diseases Impaired cognition Individual Infection Inflammation Intervention Investigation Laboratories Learning Literature Magnetic Resonance Imaging Measures Mediating Mentors Mentorship Microglia Military Personnel Molecular Myelogenous Myeloid Cells N-acetylaspartate Neopterin Neurocognitive Neurocognitive Deficit Outcome Participant Phenotype Ploidies Population Property Publishing RNA RNA I RNA Splicing Research Role Signal Transduction Sorting - Cell Movement Specimen Therapeutic Intervention Training Transcript Universities Viral Virus Replication Work antiretroviral therapy central nervous system injury chronic infection circulating biomarkers cognitive performance cohort detection limit experience improved macrophage monocyte myoinositol neurotoxicity programs response single-cell RNA sequencing telmisartan trend viral RNA

项目摘要

The goal of my work is to elucidate the role of latent HIV infection in myeloid cells as an approach to better understand myeloid immune dysfunction, inflammation and early stages of cognitive decline during acute HIV infection (AHI). Our preliminary data show monocyte associated HIV DNA data are consistent with the hypothesis that monocytes harbor HIV DNA and RNA during early infection. Expanding on our on-going studies evaluating monocyte associated HIV DNA, I intend to investigate monocyte HIV RNA burden in blood and CSF prior to and post-ART intervention and in the context of adjunctive anti-inflammatory therapy during AHI. The RV408/SEARCH018 cohort provides access to conducting my proposed single cell assessments for monocyte phenotype and single cell RNA sequencing. Results from my aims will form the basis for defining early HIV RNA transcriptional activity to our presently limited understanding of monocyte associated HIV RNA burden.

我工作的目标是阐明骨髓细胞中潜伏的HIV感染作为一种免疫抑制剂的作用。更好地了解骨髓免疫功能障碍，炎症和早期阶段的方法急性HIV感染（AHI）期间的认知能力下降。我们的初步数据显示单核细胞相关的HIV DNA数据与单核细胞携带HIV DNA的假设一致和早期感染期间的RNA。扩展我们正在进行的评估单核细胞相关的HIV DNA，我打算研究血液和CSF中的单核细胞HIV RNA负荷，在ART干预前后以及在持续性抗炎治疗期间，嗨。RV 408/SEARCH 018队列提供了进行我提出的单细胞的途径评估单核细胞表型和单细胞RNA测序。我的目标的结果这将成为我们目前有限的定义早期HIV RNA转录活性的基础。了解单核细胞相关的HIV RNA负荷。