Core B: Reagent/Service Core

核心B摘要 组成这个计划项目的三个研究项目都集中在了解分子 和导致两种主要膀胱癌(BC)膀胱肿瘤发生的细胞事件和机制 变异体和分子亚型。除了智力和实验上的协同效应，将 将单个项目整合到一个连贯的计划中，三个拟议的研究计划中的每一个都依赖于 为他们的成功提供一套通用的关键试剂和服务。这些 包括但不限于一组代表主要BC不同级别和阶段的培养细胞系 变种和分子亚型，项目负责人开发/使用的独特的膀胱癌小鼠模型 吴博士、唐博士和黄博士，核酸试剂，抗体和cdna构建。显然，它会 没有意义，也不是有效地利用时间、金钱和资源，让每个实验室 独立地产生、获取、表征和维护所有这些试剂。相反，我们已经建立了 集中的代理和服务核心，其目的之一是充当密钥的中央存储库 试剂，并为这些重要的研究工具提供集中供应，所有参与的人都可以共享这些工具 此计划项目。除了成本效益外，这种方法还具有确保 这些共享试剂在各个项目中的质量。提供细胞系，小鼠组织 来自集中核心的动物模型消除了试剂处理的可变性，便于数据 三个单独项目之间的比较。此外，它还促进了 项目负责人和防范在紧急情况下意外丢失宝贵的试剂，例如 停电(我们上一次经历停电是在2012年桑迪超级风暴期间)。最后，酷睿B还将 为个别项目提供病理和统计支持。核心的具体目标是：i) 保持集中供应，分发对支持研究工作至关重要的一套共同的核心试剂 2)收集小鼠标本并提供必要的病理学支持 服务；以及iii)提供专门的统计支持。这一核心的成功将是 这项计划项目中提出的研究。

Core B Summary The three research projects that comprise this Program Project are all focused on understanding molecular and cellular events and mechanisms leading to bladder tumorigenesis of both major bladder cancer (BC) variants and molecular subtypes. In addition to the intellectual and experimental synergies that link the individual projects into a cohesive program, each of the three proposed research plans all depend upon a common set of critical reagents and services made available on a continuous basis for their success. These include but are not limited to a set of cultured cell lines that represent different grades and stages of major BC variants and molecular subtypes, unique mouse models of bladder cancer developed/used by project leaders Dr. Wu, Dr. Tang and Dr. Huang, nucleic acid reagents, antibodies and cDNA constructs. Obviously, it would not make sense, nor would it be an efficient use of time, money, and resources for each laboratory to independently generate, procure, characterize, and maintain all these reagents. Instead, we have established a centralized Reagent and Service Core, one of whose purposes is to serve as a central repository for key reagents and provide a central supply for these important research tools that can be shared by all involved in this Program Project. In addition to the cost-effectiveness, this approach offers the advantage of ensuring the quality of these shared reagents across the individual projects. Providing supplies of cell lines, mouse tissues and animal models from a centralized core eliminates variability in reagent handling, facilitating data comparison amongst the three individual projects. Furthermore, it promotes additional interactions among the project directors and guards against accidental loss of invaluable reagents in emergency situations such as power outages (which we last experienced in 2012 during Sandy Superstorm). Finally, the Core B will also provide pathology and statistical support for the individual projects. The specific aims of the Core are: i) To maintain a centralized supply and distribute a common core set of reagents vital to support the research efforts of individual component projects; ii) To collect mouse specimens and provide the necessary pathology support service; and iii) To provide dedicated statistical support. Success of this Core will be an essential factor for the studies that have been proposed in this program project.