Core B: Reagent/Service Core

核心B总结 组成本计划项目的三个研究项目都集中在了解分子 以及导致两种主要膀胱癌（BC）的膀胱肿瘤发生的细胞事件和机制 变体和分子亚型。除了知识和实验的协同作用， 将单个项目整合为一个有凝聚力的计划，三个拟议的研究计划中的每一个都依赖于一个 一套通用的关键试剂和服务，持续提供，以确保其成功。这些 包括但不限于代表不同等级和阶段主要BC的一组培养细胞系 变体和分子亚型，项目负责人开发/使用的独特的膀胱癌小鼠模型 博士吴，唐博士和黄博士，核酸试剂，抗体和cDNA构建体。很明显， 没有意义，也不会有效地利用时间，金钱和资源，每个实验室， 独立生成、获取、表征和维护所有这些试剂。相反，我们已经建立了 一个集中的试剂和服务核心，其目的之一是作为一个中央存储库的关键 试剂，并为这些重要的研究工具提供中央供应，这些工具可以由所有参与者共享。 这个计划项目。除了成本效益之外，这种方法还提供了确保 各个项目中这些共享试剂的质量。提供细胞系、小鼠组织 和动物模型，消除了试剂处理的可变性， 三个项目之间的比较。此外，它还促进了 项目负责人和在紧急情况下防止意外丢失宝贵试剂的警卫， 停电（我们上次经历停电是在2012年的桑迪超级风暴期间）。最后，核心B还将 为个别项目提供病理学和统计学支持。该核心的具体目标是： 保持集中供应并分发一套对支持研究工作至关重要的共同核心试剂 ii）收集小鼠标本并提供必要的病理学支持 （三）提供专项统计支持。这一核心的成功将是 在这个项目中提出的研究。

Core B Summary The three research projects that comprise this Program Project are all focused on understanding molecular and cellular events and mechanisms leading to bladder tumorigenesis of both major bladder cancer (BC) variants and molecular subtypes. In addition to the intellectual and experimental synergies that link the individual projects into a cohesive program, each of the three proposed research plans all depend upon a common set of critical reagents and services made available on a continuous basis for their success. These include but are not limited to a set of cultured cell lines that represent different grades and stages of major BC variants and molecular subtypes, unique mouse models of bladder cancer developed/used by project leaders Dr. Wu, Dr. Tang and Dr. Huang, nucleic acid reagents, antibodies and cDNA constructs. Obviously, it would not make sense, nor would it be an efficient use of time, money, and resources for each laboratory to independently generate, procure, characterize, and maintain all these reagents. Instead, we have established a centralized Reagent and Service Core, one of whose purposes is to serve as a central repository for key reagents and provide a central supply for these important research tools that can be shared by all involved in this Program Project. In addition to the cost-effectiveness, this approach offers the advantage of ensuring the quality of these shared reagents across the individual projects. Providing supplies of cell lines, mouse tissues and animal models from a centralized core eliminates variability in reagent handling, facilitating data comparison amongst the three individual projects. Furthermore, it promotes additional interactions among the project directors and guards against accidental loss of invaluable reagents in emergency situations such as power outages (which we last experienced in 2012 during Sandy Superstorm). Finally, the Core B will also provide pathology and statistical support for the individual projects. The specific aims of the Core are: i) To maintain a centralized supply and distribute a common core set of reagents vital to support the research efforts of individual component projects; ii) To collect mouse specimens and provide the necessary pathology support service; and iii) To provide dedicated statistical support. Success of this Core will be an essential factor for the studies that have been proposed in this program project.