Analytical Imaging

分析成像

• 批准号: 10228627
• 负责人: JOHN S CONDEELIS
• 金额: $ 16.88万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10228627
• 关键词: 3-Dimensional; Albert Einstein Cancer Center; Animals; Array tomography; Biological Models; Cancer Center; Cell Culture Techniques; Cells; Communities; Complex; Computer software; Confocal Microscopy; Cryoelectron Microscopy; Data Analyses; Educational workshop; Electron Microscopy; Equipment; Fluorescence; Fluorescence Microscopy; Freezing; Funding; Goals; Gold; Grant; Growth; Hydration status; Image; Image Analysis; Imaging Techniques; Imaging technology; Individual; Label; Laboratories; Location; Maintenance; Malignant Neoplasms; Microscope; Microscopy; Molecular; Organ; Phase; Preparation; Procedures; Proteins; Reporter Genes; Research; Research Personnel; Resolution; Resource Sharing; Resources; Sampling; Scanning; Scanning Electron Microscopy; Science; Services; Site; Slide; Specimen; Spectrometry, Mass, Secondary Ion; Speed; Technical Expertise; Techniques; Technology; Time; Tissues; Training; United States National Institutes of Health; Visualization; cellular imaging; college; cost effective; electron tomography; experience; faculty research; image processing; imaging facilities; imaging modality; imaging platform; innovation; instrument; instrumentation; light microscopy; live cell microscopy; macromolecule; member; microscopic imaging; multiphoton microscopy; new technology; operation; pressure; programs; quantitative imaging; reconstruction; serial imaging; spectrograph; transmission process

PROJECT SUMMARY – ANALYTICAL IMAGING FACILITY SHARED RESOURCE The Analytical Imaging Facility (AIF) is a comprehensive microscopy shared resource that offers a broad spectrum of imaging technologies to AECC investigators. These include: macro-imaging with a stereoscope, classical microscopy including brightfield, darkfield, phase contrast, Nomarski differential interference contrast and wide-field epifluorescence, confocal microscopy for cell culture and tissues, high-speed and high-capacity automatic slide scanning, high speed spinning disk confocal microscopy for live cells, intravital multiphoton microscopy for live animals, super-resolution STORM and SIM microscopy, standard transmission and scanning electron microscopy (TEM & SEM), cryoEM of macromolecules, electron tomography and 3D reconstruction of high pressure frozen cells and tissue, automated imaging of serial sections by field emission SEM (FESEM) and SEM of frozen hydrated cells and tissues. The wide range of imaging modalities is especially important for studying the diverse biological models utilized by the AECC research community. For instance, cells labeled with fluorescent reporter genes may be imaged at the whole animal or whole organ level down to subcellular and molecular levels. Over the span of 23 years, the AIF has expanded its dedicated laboratory space and staff, and added multiple new instruments and equipment upgrades. This growth has been enabled by funding from Cancer Center and College and Shared Instruments Grants. This facility furnishes AECC investigators with the majority of their on-site access to routine and sophisticated imaging technologies. The technologies provided are essential to the research of many AECC members, and cannot be outsourced or obtained at other sites in any practical or cost effective way. Having the technology and staff in one location provides continuity, facilitates cross-training and adds value to the AECC researchers who use microscopy. Beyond making routine and complex imaging technologies available to the AECC community, the AIF also provides comprehensive training in most technologies so that AECC members and their staff are competent in the operations of the equipment, image analysis and the interpretation of the data acquired. This is accomplished by supporting routine as well as advanced microscopy applications, training users in microscopy and image analysis software, hosting regular workshops on microscopy and image processing and introducing innovative imaging technologies developed at this center to meet the needs of AECC investigators.

项目摘要-分析成像设备共享资源 分析成像设备(AIF)是一个全面的显微镜共享资源，它提供了广泛的 向AECC调查人员介绍成像技术的范围。其中包括：用立体显微镜进行宏观成像， 经典显微镜包括明场、暗场、位相对比度、诺马尔斯基微分干涉对比度 和广域荧光，用于细胞培养和组织的共聚焦显微镜，高速和大容量 自动载玻片扫描，活细胞高速旋转圆盘共聚焦显微镜，活体多光子 活体动物显微镜，超分辨率STORM和SIM显微镜，标准透射式和 扫描电子显微镜、大分子低温电子显微镜、电子断层扫描和三维成像 高压冷冻细胞和组织的重建，场发射连续切片的自动成像 扫描电子显微镜(FESEM)和冷冻水化细胞和组织的扫描电子显微镜。成像方式的广泛范围包括 对于研究AECC研究界使用的各种生物模型尤其重要。为 例如，标记有荧光报告基因的细胞可以在整个动物或整个器官水平上成像 深入到亚细胞和分子水平。在23年的时间里，AIF扩大了其致力于 增加了实验室空间和工作人员，并增加了多台新仪器和设备升级。这种增长已经 由癌症中心、大学和共享仪器基金资助。这个设施 为AECC调查人员提供了大部分现场访问常规和复杂成像的机会 技术。所提供的技术对许多AECC成员的研究是必不可少的，不能 以任何实际或具有成本效益的方式外包或在其他地点获得。拥有技术和员工 一个位置提供了连续性，促进了交叉培训，并为使用 显微镜。除了向AECC社区提供常规和复杂的成像技术外， AIF还提供大多数技术的全面培训，以便AECC成员及其工作人员 能够胜任设备的操作、图像分析和对采集的数据进行解释。这 是通过支持常规和高级显微镜应用程序、培训用户 显微镜和图像分析软件，定期举办关于显微镜和图像处理的研讨会和 介绍该中心开发的创新成像技术，以满足AECC调查人员的需求。