Effect of Drugs of Abuse on CNS HIV-1 Reservoirs and Neuropathogenesis

滥用药物对中枢神经系统 HIV-1 病毒库和神经发病机制的影响

基本信息

  • 批准号:
    10419775
  • 负责人:
  • 金额:
    $ 0.95万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2016
  • 资助国家:
    美国
  • 起止时间:
    2016-09-01 至 2022-07-31
  • 项目状态:
    已结题

项目摘要

PROJECT SUMMARY The long term goal of this application is to study HIV-1 reservoirs that persist in CNS and to understand the role of illicit drugs in establishing and reactivating the latent reservoirs. Persistence of latently infected cells in CNS poses a major barrier for HIV-1 eradication. Current strategies to eliminate the latent reservoirs include a “shock and kill” therapy and is aimed at peripheral blood, which constitutes only 1% of the total reservoirs. Whether it is possible to envision similar strategies for the eradication of HIV-infected CNS cells is currently unknown. In addition to the lack of knowledge about latency in CNS HIV+ cells and the effects of latency reversing agents, illicit drugs common within the HIV-infected populations constitute a further complexity. Many illicit drugs are known to stimulate HIV-1 replication. Since the current method to measure the peripheral blood HIV reservoir is not applicable to solid tissues or to cells that replicate poorly such as macrophages, microglia and astrocytes found in the brain. We have developed a novel, Single cell-single molecule, Multiplex, Immunofluorescence (IF) and RNA FISH-based Assay (SMIRA) to detect cells in which HIV-1 is actively replicating. Using automation, a large number of cells can be scanned. In this proposal, we will employ this novel method to first quantitate and characterize latency in cell line models, then the latent reservoirs in brain derived microglia and astrocytes and delineate the effect of three drugs of abuse (methamphetamine, cocaine and cannabis) on the efficiency of formation of latent cells, the rate of reactivation of latent cells, and establishment of latency across blood-brain-barrier (BBB). For the purpose of the summer undergraduate program, we will be studying the role of illicit drugs on the reactivation of latently infected CNS-derived cell lines by establishing in vitro models of latency. We will employ immortalized human monocyte and microglial cell lines to study the kinetics of reactivation and the effect of METH using SMIRA.
项目总结

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

GANJAM V KALPANA其他文献

GANJAM V KALPANA的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('GANJAM V KALPANA', 18)}}的其他基金

RNA-mimicry to guide the intra-cellular targeting of host virus protein and viral RNA-protein interactions to inhibit HIV replication.
RNA模拟引导宿主病毒蛋白的细胞内靶向和病毒RNA-蛋白相互作用以抑制HIV复制。
  • 批准号:
    10554025
  • 财政年份:
    2022
  • 资助金额:
    $ 0.95万
  • 项目类别:
RNA-mimicry to guide the intra-cellular targeting of host virus protein and viral RNA-protein interactions to inhibit HIV replication.
RNA模拟引导宿主病毒蛋白的细胞内靶向和病毒RNA-蛋白相互作用以抑制HIV复制。
  • 批准号:
    10618961
  • 财政年份:
    2022
  • 资助金额:
    $ 0.95万
  • 项目类别:
Single cell RNA-seq and single molecule RNA-FISH approaches to study stochasticity of latent HIV-1 reactivation
单细胞 RNA-seq 和单分子 RNA-FISH 方法研究潜在 HIV-1 重新激活的随机性
  • 批准号:
    10082908
  • 财政年份:
    2020
  • 资助金额:
    $ 0.95万
  • 项目类别:
Structure-based design of stapled peptides to target Gag-Pol and INI1 interaction to block assembly
基于结构的钉合肽设计,以靶向 Gag-Pol 和 INI1 相互作用来阻止组装
  • 批准号:
    10302316
  • 财政年份:
    2020
  • 资助金额:
    $ 0.95万
  • 项目类别:
Mechanism of HIV-1 Latency and Reactivation Kinetics Using Single Cell Analysis
使用单细胞分析研究 HIV-1 潜伏期和再激活动力学的机制
  • 批准号:
    9298589
  • 财政年份:
    2016
  • 资助金额:
    $ 0.95万
  • 项目类别:
Effect of drugs of abuse on CNS HIV-1 reservoirs and neuropathogenesis
滥用药物对 CNS HIV-1 储存库和神经发病机制的影响
  • 批准号:
    9532834
  • 财政年份:
    2016
  • 资助金额:
    $ 0.95万
  • 项目类别:
Effect of drugs of abuse on CNS HIV-1 reservoirs and neuropathogenesis
滥用药物对 CNS HIV-1 储存库和神经发病机制的影响
  • 批准号:
    9333307
  • 财政年份:
    2016
  • 资助金额:
    $ 0.95万
  • 项目类别:
Effect of drugs of abuse on CNS HIV-1 reservoirs and neuropathogenesis
滥用药物对 CNS HIV-1 储存库和神经发病机制的影响
  • 批准号:
    9977143
  • 财政年份:
    2016
  • 资助金额:
    $ 0.95万
  • 项目类别:
Mechanism of HIV-1 Latency and Reactivation Kinetics Using Single Cell Analysis
使用单细胞分析研究 HIV-1 潜伏期和再激活动力学的机制
  • 批准号:
    9207956
  • 财政年份:
    2016
  • 资助金额:
    $ 0.95万
  • 项目类别:
Effect of drugs of abuse on CNS HIV-1 reservoirs and neuropathogenesis
滥用药物对 CNS HIV-1 储存库和神经发病机制的影响
  • 批准号:
    9926378
  • 财政年份:
    2016
  • 资助金额:
    $ 0.95万
  • 项目类别:

相似海外基金

Linking Epidermis and Mesophyll Signalling. Anatomy and Impact in Photosynthesis.
连接表皮和叶肉信号传导。
  • 批准号:
    EP/Z000882/1
  • 财政年份:
    2024
  • 资助金额:
    $ 0.95万
  • 项目类别:
    Fellowship
Digging Deeper with AI: Canada-UK-US Partnership for Next-generation Plant Root Anatomy Segmentation
利用人工智能进行更深入的挖掘:加拿大、英国、美国合作开发下一代植物根部解剖分割
  • 批准号:
    BB/Y513908/1
  • 财政年份:
    2024
  • 资助金额:
    $ 0.95万
  • 项目类别:
    Research Grant
Simultaneous development of direct-view and video laryngoscopes based on the anatomy and physiology of the newborn
根据新生儿解剖生理同步开发直视喉镜和视频喉镜
  • 批准号:
    23K11917
  • 财政年份:
    2023
  • 资助金额:
    $ 0.95万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Genetics of Extreme Phenotypes of OSA and Associated Upper Airway Anatomy
OSA 极端表型的遗传学及相关上呼吸道解剖学
  • 批准号:
    10555809
  • 财政年份:
    2023
  • 资助金额:
    $ 0.95万
  • 项目类别:
computational models and analysis of the retinal anatomy and potentially physiology
视网膜解剖学和潜在生理学的计算模型和分析
  • 批准号:
    2825967
  • 财政年份:
    2023
  • 资助金额:
    $ 0.95万
  • 项目类别:
    Studentship
Computational comparative anatomy: Translating between species in neuroscience
计算比较解剖学:神经科学中物种之间的翻译
  • 批准号:
    BB/X013227/1
  • 财政年份:
    2023
  • 资助金额:
    $ 0.95万
  • 项目类别:
    Research Grant
Doctoral Dissertation Research: Social and ecological influences on brain anatomy
博士论文研究:社会和生态对大脑解剖学的影响
  • 批准号:
    2235348
  • 财政年份:
    2023
  • 资助金额:
    $ 0.95万
  • 项目类别:
    Standard Grant
Development of a novel visualization, labeling, communication and tracking engine for human anatomy.
开发一种新颖的人体解剖学可视化、标签、通信和跟踪引擎。
  • 批准号:
    10761060
  • 财政年份:
    2023
  • 资助金额:
    $ 0.95万
  • 项目类别:
Understanding the functional anatomy of nociceptive spinal output neurons
了解伤害性脊髓输出神经元的功能解剖结构
  • 批准号:
    10751126
  • 财政年份:
    2023
  • 资助金额:
    $ 0.95万
  • 项目类别:
The Anatomy of Online Reviews: Evidence from the Steam Store
在线评论剖析:来自 Steam 商店的证据
  • 批准号:
    2872725
  • 财政年份:
    2023
  • 资助金额:
    $ 0.95万
  • 项目类别:
    Studentship
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了