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Pathology and Biochemistry Core

病理学和生物化学核心

基本信息

批准号：
10424902
负责人：
KATHRYN J MOORE
金额：
$ 28.83万
依托单位：
NEW YORK UNIVERSITY SCHOOL OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-05-01 至 2027-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10424902
关键词：
Adipocytes Adipose tissue Advanced Glycosylation End Products Antigens Aorta Apoptosis Area Arterial Fatty Streak Atherosclerosis Biochemical Biochemistry Biological Assay Blood Blood Circulation Bone Marrow Stem Cell Cardiometabolic Disease Cell Nucleus Cells Cholesterol Collagen Cross-Sectional Studies Cultured Cells Dental crowns Detection Digestion Ensure Fatty acid glycerol esters Fibrosis Functional disorder Gene Expression Goals Hepatic High Density Lipoproteins Histologic Histology Immune Individual Inflammation Inflammation Mediators Inflammatory Inflammatory Response Label Lipids Liver Low-Density Lipoproteins Measurement Measures Metabolic Metabolic dysfunction Monitor Morphology Mus Necrosis Obesity Pathologic Processes Pathology Pathway interactions Plant Roots Plasma Population Procedures Productivity Reproducibility Scientist Services Sirius Red F3B Stains Standardization Structure Tissues Triglycerides Very low density lipoprotein antigen detection cardiometabolism cell type dietary fast protein liquid chromatography insight liver injury macrophage monocyte non-alcoholic fatty liver disease programs protein expression single-cell RNA sequencing stem cells transcriptome sequencing transcriptomics

项目摘要

PROJECT SUMMARY: CORE B Accumulation of inflammatory macrophages as well as innate and adaptative immune cells is a common feature of cardiometabolic diseases, including atherosclerosis, obesity and non-alcoholic fatty liver diseases. Inflammation in metabolic tissues accelerates atherosclerosis and metabolic dysfunction. The ability to examine changes in gene expression in immune cells, particularly macrophage populations, in the plaque, adipose tissue depots, and liver during cardiometabolic disease provides insights into that key pathways that promote or inhibit the inflammatory response and their corresponding effects on pathologic processes in these tissues. As such, a Core will be established to: 1) isolate and quantify macrophage and other immune cell subsets from atherosclerotic plaque, adipose tissue and liver by FACS; 2) monitor changes in metabolic parameters within the systemic circulation, atherosclerotic plaque, adipose tissue and liver; and 3) perform sectioning and histologic quantification of atherosclerotic plaques, fat depots and liver morphology. These procedures are key to the successful execution of the Aims of all three projects in this Program Project, and centralizing the immunohistochemical and biochemical assays will ensure consistency and reproducibility, which in turn will maximize the efficiency and productivity of the scientists within each project.

项目总结：核心b