Core B: Neuroscience Core

核心 B：神经科学核心

• 批准号: 10445014
• 负责人: Huxing Cui
• 金额: $ 15.77万
• 依托单位: MEDICAL COLLEGE OF WISCONSIN
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10445014
• 关键词: Adipose tissue; Adrenergic Fibers; Affect; Biological Models; Blood; Brain; Brain region; Cardiovascular Physiology; Cardiovascular system; Clozapine; Control Groups; Data; Data Analyses; Energy Metabolism; Ensure; Equipment; Equipment and Supplies; Fiber; Fiber Optics; Gene Expression; Genetic; Goals; Histologic; Human Resources; Hypothalamic structure; Immunohistochemistry; Injections; Kidney; Lasers; Ligands; Metabolic; Methods; Microinjections; Molecular; Muscle; Nerve; Neurosciences; Operative Surgical Procedures; Oxides; Preparation; Proteins; Protocols documentation; Regulation; Reproducibility; Research Personnel; Resources; Signal Transduction; Structure of nucleus infundibularis hypothalami; Subfornical Organ; Techniques; Training; Validation; Viral Vector; cardiometabolism; cost effective; data dissemination; delivery vehicle; design; designer receptors exclusively activated by designer drugs; experimental study; genetic manipulation; instrumentation; mRNA Expression; microscopic imaging; mind control; neural circuit; neuromechanism; neurophysiology; optogenetics; paraventricular nucleus; programs; protein expression

Summary / Abstract The objective of the current program is to advance our understanding of the molecular and neurocircuitry mechanisms within the brain that control cardiovascular function and energy metabolism. The Neuroscience Core is designed to provide critical equipment and technical support for studying neural mechanisms affecting cardiovascular and metabolic functions that will be employed by all four projects within the program. Investigators will have a full access to an array of equipment through this core to achieve the goal of proposed studies that involve brain-region-specific gene manipulation, chemogenetic approach, multi-fiber recording of regional sympathetic nerve activity, and histological examination of the brain. In addition, the Directors and core staff will provide all necessary surgical support and training for all projects. The centralization of these endpoints into a core will ensure scientific rigor and reproducibility of all proposed experiments that will use advanced neuroscience techniques across the program.

摘要/摘要 目前的计划的目标是推进我们的分子和神经回路的理解 大脑中控制心血管功能和能量代谢的机制。神经科学 核心是为了提供关键设备和技术支持，研究神经机制， 心血管和代谢功能，将在该计划的所有四个项目。 调查人员将有一个完整的访问阵列的设备，通过这个核心，以实现拟议的目标， 涉及脑区特异性基因操作、化学遗传学方法、多纤维记录 区域交感神经活动和脑组织学检查。此外，董事和 核心工作人员将为所有项目提供一切必要的手术支助和培训。这些集中化的 将端点纳入核心将确保所有拟议实验的科学严谨性和可重复性， 先进的神经科学技术