Developing and validating serological assays to SARS-COV-2 for highly susceptible animal species

项目摘要 SARS-CoV-2是一种有包膜的正义RNA病毒，已知可与血管紧张素- 转化酶-2（ACE 2）受体，包括人、猫、狗、貂、雪貂、鹿， 等人自2019年底在中国武汉出现以来，SARS-CoV-2，COVID-19的病原体， 19，已被列为大流行病，对公共卫生造成严重破坏，并导致全球封锁。 由于它的突然出现，以及它与SARS-CoV-1和MERS-CoV的相似性，人们怀疑， SARS-CoV-2可能有一个中间宿主动物。然而，ACE 2受体的相似性， 冠状病毒引发了哪些动物物种易感染SARS-CoV-2的问题， 能够通过病毒传播在其中一些物种中描述了反向人畜共患病，表明， 人与动物之间的相互作用可能对SARS-CoV-2感染造成双重风险。因此，关键是 为动物进行准确的诊断血清学测试，以了解病毒在动物中的流行情况 人口该项目将比较内部开发的商业ELISA之间的诊断结果 ELISA和SARS-CoV-2的血清中和试验，以及针对其他 冠状病毒以排除交叉反应。这项研究的结果将提供洞察的准确性， (Se/Sp）市售血清学检测、新开发的内部血清学技术，以及 收集目标物种中SARS-CoV-2流行率的数据，包括水貂、雪貂、白尾鹿， 家养的狗和猫、牛、绵羊和山羊。

Project Summary SARS-CoV-2 is an enveloped, positive-sense RNA virus that is known to interact with the angiotensin- converting enzyme-2 (ACE2) receptor of several species, including humans, cats, dogs, minks, ferrets, deer, and others. Since its emergence in Wuhan, China in late 2019, SARS-CoV-2, the causative agent of COVID- 19, has been classified as a pandemic, causing havoc on public health and resulting in worldwide lockdowns. Due to its sudden emergence, as well as its similarity to SARS-CoV-1 and MERS-CoV, it is suspected that SARS-CoV-2 likely had an intermediate animal host. However, the similarities in the ACE2 receptors of coronaviruses have sparked the question of which animal species are susceptible to SARS-CoV-2, and capable of viral transmission. Reverse-zoonosis has been described in a few of these species, indicating that human-animal interactions can pose two-sided risks for SARS-CoV-2 infection. Therefore, it is critical to produce accurate diagnostic serological tests for animals to understand prevalence of the virus in the animal population. This project will compare diagnostic results between commercial ELISAs, developed in-house ELISAs, and serum neutralization assays for SARS-CoV-2, as well as Western blot against other coronaviruses to rule out cross-reactivity. The outcome of this study will provide insight into the accuracy (Se/Sp) of commercially available serological tests, newly developed in-house serological techniques, and collect data on SARS-CoV-2 prevalence in target species, which will include minks, ferrets, white-tailed deer, domestic dogs and cats, cows, sheep, and goats.