Developing and validating serological assays to SARS-COV-2 for highly susceptible animal species

项目摘要 SARS-CoV-2是一种有包膜的阳性RNA病毒，已知与血管紧张素-2相互作用。 转换酶-2(ACE2)受体的几个物种，包括人，猫，狗，貂，雪貂，鹿， 还有其他人。自2019年底在武汉出现以来，中国、冠状病毒病原体-- 19，已被列为大流行，对公共卫生造成严重破坏，并导致全球封锁。 由于它的突然出现，以及它与SARS-CoV-1和MERS-CoV的相似性，人们怀疑 SARS-CoV-2可能有一个中间动物宿主。然而，ACE2受体的相似性 冠状病毒引发了哪些动物物种对SARS-CoV-2易感的问题，以及 能够通过病毒传播的。在这些物种中的一些物种中已经描述了反向人畜共患病，这表明 人与动物的相互作用可能会给SARS-CoV-2感染带来双向风险。因此，至关重要的是 为动物制作准确的诊断血清学测试，以了解病毒在动物体内的流行情况 人口。该项目将比较内部开发的商用ELISA的诊断结果 ELISA，SARS-CoV-2的血清中和试验，以及针对其他 冠状病毒排除交叉反应。这项研究的结果将提供对准确性的洞察 (Se/Sp)市场上可获得的血清学检测，新开发的内部血清学技术，以及 收集SARS-CoV-2在目标物种中流行的数据，包括水貂、雪貂、白尾鹿、 家养的狗和猫、牛、绵羊和山羊。

Project Summary SARS-CoV-2 is an enveloped, positive-sense RNA virus that is known to interact with the angiotensin- converting enzyme-2 (ACE2) receptor of several species, including humans, cats, dogs, minks, ferrets, deer, and others. Since its emergence in Wuhan, China in late 2019, SARS-CoV-2, the causative agent of COVID- 19, has been classified as a pandemic, causing havoc on public health and resulting in worldwide lockdowns. Due to its sudden emergence, as well as its similarity to SARS-CoV-1 and MERS-CoV, it is suspected that SARS-CoV-2 likely had an intermediate animal host. However, the similarities in the ACE2 receptors of coronaviruses have sparked the question of which animal species are susceptible to SARS-CoV-2, and capable of viral transmission. Reverse-zoonosis has been described in a few of these species, indicating that human-animal interactions can pose two-sided risks for SARS-CoV-2 infection. Therefore, it is critical to produce accurate diagnostic serological tests for animals to understand prevalence of the virus in the animal population. This project will compare diagnostic results between commercial ELISAs, developed in-house ELISAs, and serum neutralization assays for SARS-CoV-2, as well as Western blot against other coronaviruses to rule out cross-reactivity. The outcome of this study will provide insight into the accuracy (Se/Sp) of commercially available serological tests, newly developed in-house serological techniques, and collect data on SARS-CoV-2 prevalence in target species, which will include minks, ferrets, white-tailed deer, domestic dogs and cats, cows, sheep, and goats.