Building testing capacity for past and current SARS-CoV-2 infection in animals with potential exposure to humans with COVID-19

项目摘要 该提案的目标是在威斯康星州兽医诊断中心建立检测能力。 进行血清学检测和全基因组测序的实验室- 动物样本中的CoV-2。我们正在申请购买试剂的资金， 用于验证SARS-CoV-2替代病毒中和检测试剂盒（GenScript）作为 多物种诊断血清学检测，特别关注抗体检测 抗SARS-CoV-2病毒。作为拟议的基因组联盟的一部分， 我们正在申请资金购买QIAxcel Adnavced系统（Qiagen）和MinION （Oxford Nanopore）增强包，用于验证iSeq的文库制备和测序试剂盒 （Illumina）和MinION用于靶向SARS-CoV-2测序。我们将参加 开发和验证生物信息学管道， 兽医诊断人员快速有效地确定基因组谱系，以及 制定员工培训和发展计划，以保持测序的熟练程度， 来分析SARS-COV-2的基因组我们的目标是建立一个经过验证的多物种 血清学诊断分析和经过培训的工作人员，简化全基因组工作流程 在动物标本中检测到的SARS-CoV-2病毒的序列，以提高我们的能力， 支持对正在进行的全球SARS-CoV-2大流行的诊断活动的能力。反过来， 获得的设备和训练将有助于建立WVDL的准备，以应对 未来可能爆发的其他重要兽医疾病， 食品安全和动物福利。

Project Abstract The goal of this proposal is to build testing capacity at the Wisconsin Veterinary Diagnostic Laboratory (WVDL) to perform serology detection and whole genome sequencing of SARS- CoV-2 from animal samples. We are requesting funding for the purchase of reagents and equipment to validate the SARS-CoV-2 Surrogate Virus Neutralization Test kit (GenScript) as a multi-species diagnostic serology assay, with specific focus on the detection of antibodies against SARS-CoV-2 virus in companion animals. As part of a proposed Genomic Consortium, we are requesting funds to purchase a QIAxcel Adnavced System (Qiagen) and a MinION (Oxford Nanopore) Enhanced Pack to validate library preparation and sequencing kits for iSeq (Illumina) and MinION for targeted SARS-CoV-2 sequencing. We will participate in the development and validation of bioinformatics pipelines that will be made accessible to veterinary diagnostic personnel to quickly and efficiently determine genomic lineages, and develop a staff training and development program to maintain proficiency in sequencing and to analyze the SARS-COV-2 genome. Together, we aim to have a validated multi-species serological diagnostic assay and trained staff with streamline workflow for whole genome sequence of the SARS-CoV-2 virus detected in animal specimens, to increase our capacity and capability to support diagnostic activities for the ongoing global SARS-CoV-2 pandemic. In turn, the acquired equipment and training will help building WVDL’s readiness to response to potentially other important future veterinary disease outbreaks that are important to human food safety and animal welfare.