Microscopy

显微镜检查

• 批准号: 10454165
• 负责人: Deanna L Benson
• 金额: $ 11.78万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-01 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10454165
• 关键词: Biological; Calcium; Cancer Center Support Grant; Cell Communication; Cell Count; Cell Lineage; Cells; Characteristics; Collaborations; Computer software; Computers; Consult; Consultations; Cultured Cells; Development; Doctor of Philosophy; Educational workshop; Electron Microscopy; Engineering; Fluorescence; Fluorescence Resonance Energy Transfer; Funding; Grant; Hour; Image; Image Analysis; Imaging Device; Immunofluorescence Immunologic; Institutes; International; Laboratories; Lead; Light; Malignant Neoplasms; Methodology; Microscope; Microscopy; Mission; Modality; Neoplasm Metastasis; Organ; Organoids; Paper; Primary Neoplasm; Productivity; Proteins; Protocols documentation; Publications; Publishing; Quantitative Microscopy; Recording of previous events; Research; Research Personnel; Resolution; Resource Sharing; Resources; Role; Schools; Science; Scientist; Series; Services; Signal Transduction; Site; Specialist; Speed; System; Techniques; Time; Tissues; Training; Training and Education; Transmission Electron Microscopy; Tumor Cell Migration; Tumor-infiltrating immune cells; Vendor; Work; anticancer research; base; cell behavior; cell growth; computerized data processing; cost; design; equipment training; experience; experimental study; gigabyte; imaging approach; imaging study; improved; in vivo; in vivo imaging; instrument; instrumentation; intravital imaging; light microscopy; medical schools; medical specialties; member; neoplastic cell; new technology; operation; particle; programs; protein expression; skills training; training opportunity; tumor diagnostic

PROJECT SUMMARY/ABSTRACT The Tisch Cancer Institute (TCI) Microscopy Shared Resource Facility (MSRF) provides TCI members access to equipment, training, expert consultation, and collaborative opportunities across a full-range of light and electron microscopy applications, including: confocal, multiphoton, super-resolution, in vivo imaging, light sheet, widefield, and transmission electron microscopy (EM). Three high-end workstations equipped with state-of-the- art software accompanied by a local server and high-speed connectivity permit a wide range of image analysis and quantification strategies. Five full time scientists staff the MSRF during regular hours and provide real-time assistance to both novice and experienced users. All of the MSRF scientists can be tapped for implementing advanced approaches. Each holds a PhD or was otherwise trained intensively in a field having particular relevance to their imaging specialty such as super-resolution microscopy or computer engineering. In particular, the EM specialist is a leader in the field and has published over ninety papers covering everything from correlative light-EM (CLEM) to traditional EM techniques, and the scientific advisor is also a TCI member who is expert in intravital imaging approaches and works collaboratively with novice TCI users needing to apply this strategy to cancer-related questions. The MSRF also sponsors monthly seminars featuring scientists using microscopy or vendors developing relevant new technology and its scientists co-direct an annual hands-on microscopy course for graduate school trainees and host workshops throughout the year. The capabilities of the microscope systems together with the skills and training of the team are crucial for supporting TCI studies that include multichannel immunofluorescence for tumor diagnostics, localization of cell signaling and growth regulatory molecules, tumor cell migration and invasion, and cell biological mechanisms of metastasis and dormancy.

项目摘要/摘要 Tisch癌症研究所(TCI)显微共享资源设施(MSRF)为TCI成员提供访问 到设备、培训、专家咨询和全方位的轻型和 电子显微镜应用，包括：共焦、多光子、超分辨率、活体成像、光片、 Widefield和透射电子显微镜(EM)。三台高端工作站，配备最先进的 配有本地服务器和高速连接的ART软件允许进行广泛的图像分析 和量化策略。五名全职科学家在正常时间为MSRF工作，并提供实时 为新手和有经验的用户提供帮助。所有MSRF的科学家都可以被任命来实施 先进的方法。每个人都拥有博士学位或以其他方式在特定领域接受过密集培训 与他们的成像专业相关，如超分辨率显微镜或计算机工程。特别是， 这位EM专家是该领域的领先者，发表了90多篇论文，涵盖了Related Light-EM(Clem)到传统的EM技术，科学顾问也是TCI成员，他是 生命中成像方法，并与需要应用此策略的TCI新手用户协作 与癌症相关的问题。MSRF还每月举办研讨会，让科学家使用显微镜或 开发相关新技术的供应商和它的科学家共同指导每年一次的动手显微镜课程 为研究生院实习生提供培训，并全年举办研讨会。显微镜的性能 系统以及团队的技能和培训对于支持TCI研究至关重要，这些研究包括 多通道免疫荧光在肿瘤诊断、细胞信号定位和生长调节中的应用 分子，肿瘤细胞迁移和侵袭，转移和休眠的细胞生物学机制。