LUMICKS's C-Trap

项目摘要 本提案的目标是获得一种最先进的光镊仪器结合多色 单分子成像，用于最近在Gruss内创建的单分子分析核心（CSMA）， 理柏生物光子学中心（GLBP）在阿尔伯特·爱因斯坦医学院（爱因斯坦）。具体来说，我们建议 制造LUMICKS的C-Trap（双光镊结合三色共焦成像和多通道 微流控技术）提供给爱因斯坦的研究人员通过专家培训，咨询和合作。 由于光镊与多色荧光显微镜的集成，C-Trap允许相关的 力（机械传感）和荧光测量进行，使广泛的调查 一系列生物过程，包括DNA和RNA-蛋白质相互作用的单分子研究 （转录、DNA和RNA复制以及DNA修复）、翻译、蛋白酶体降解、时相 分离和免疫学，仅举几个研究领域。在CSMA中放置C-Trap将极大地 加强和加速NIH资助的爱因斯坦项目，其中许多是理想的单分子研究 并将从这项技术中受益匪浅。特别是，C-Trap将用于研究 微管转运系统的调节，染色质组织和功能，转录调节， DNA复制和修复、RNA编辑和细菌运动性。因此，所要求的技术将打开 与癌症相关的生物学途径研究的新可能性-包括候选人的测试 治疗，并使初级教师写R 01赠款申请，否则不能写。在 这样，C阱将使爱因斯坦站在创新的单分子癌症研究的最前沿， 从而提高人们对爱因斯坦及其教职员工，特别是爱因斯坦癌症中心的认识。短短四 年前，诺贝尔物理学奖授予了亚瑟阿什金，因为他发明了光镊， 强调这项技术的创新性质和这项建议的及时性。在制作C- 陷阱提供给癌症生物学家在爱因斯坦，新的癌症疗法和治疗方法可以开发， 将有益于人类健康。

Project Summary The goal of this proposal is to acquire a state-of-the-art optical tweezers instrument combined with multi-color single-molecule imaging for the recently created Core for Single-Molecule Analysis (CSMA) within the Gruss- Lipper Biophotonics Center (GLBP) at the Albert Einstein College of Medicine (Einstein). Specifically, we propose to make LUMICKS’ C-Trap (dual optical tweezers combined with 3-color confocal imaging and multi-channel microfluidics) available to the researchers of Einstein through expert training, consultation and collaborations. Due to the integration of optical tweezers with multi-color fluorescence microscopy, the C-Trap allows correlative force (mechanosensing) and fluorescence measurements to be performed, enabling investigation of a wide range of biological processes, including single-molecule studies on DNA- and RNA-protein interactions (transcription, DNA and RNA replication, and DNA repair), translation, proteasomal degradation, phase separation, and immunology, to name a few areas of research. Placement of the C-Trap in the CSMA will greatly enhance and accelerate NIH-funded projects at Einstein, many of which are ideal for single-molecule studies and would greatly benefit from access to this technology. In particular, the C-Trap will be used to study the regulation of the microtubule transport system, chromatin organization and function, transcriptional regulation, DNA replication and repair, RNA editing, and bacterial motility. The requested technology will therefore open up novel possibilities for research on biological pathways relevant to cancer—including the testing of candidate therapeutics—and enable junior faculty to write R01 grant applications that could otherwise not be written. In doing so, the C-Trap will place Einstein at the forefront of innovative, single-molecule cancer research and thereby increase the recognition of Einstein, its faculty, and the Einstein Cancer Center in particular. Just four years ago, the Nobel prize for physics was awarded to Arthur Ashkin for the invention of optical tweezers, highlighting the innovative nature of this technology and the timely manner of this proposal. In making the C- Trap available to cancer biologists at Einstein, new cancer therapies and treatments can be developed, which will benefit human health.