Experimental Tumor Model Shared Resource

由于CCSG的战略规划和所提供的服务评估，以前的小鼠肿瘤模型 共享资源(MTM)更名为实验肿瘤模型共享资源(ETM)。ETM有 扩展了其实验模型，包括来自患者的异种移植物、器官模型和球体模型 样本。主要目标是提供高质量、高成本效益的定制服务，以满足以下需求 个人研究人员，并提供实验模型和技术专门知识的综合资源 为活体实验的所有方面提供支持，以支持CCSG研究人员的研究努力。ETM共享 RESOURCE总共为65名罗斯威尔用户服务，其中60名(92%)是CCSG成员。ETM是一项巨大的价值 他们的用户，因为没有商业选择，为活体研究提供全方位的服务， 由ETM提供。用于生产动物的巩固的繁育群体提供了巨大的节省 通过商业渠道购买转基因小鼠。ETM资源有两个主要领域： 焦点：(1)利用癌症模型的实验研究，包括维持模型；组织生物库 并为治疗、反应和组织的评估提供技术支持 采购；和(Ii)小鼠组织的组织学服务。ETM服务包括全频谱启动 随着研究方案的发展，提供实验模型，对组织进行体内治疗 采用标准化小鼠免疫组织化学方法进行组织学染色。提供的组织学服务 ETM的重点是小鼠组织。该资源维护着几个广泛使用的型号的繁育群体 前列腺癌。ETM还维护了肉瘤、肺、胰腺的转基因模型(在 开发)，以及致癌物诱导的膀胱癌模型。ETM建立并维护 各种患者来源的异种移植(PDX)模型。ETM的具体目标是：1)提供一个 利用癌症实验模型进行体内研究的综合资源，包括 为活体研究提供生物标本、实验模型和技术支持；2)提供组织 为涉及实验模型的研究提供采购和组织学支持。ETM计划建立新的 从临床标本中建立PDX模型；用深分子技术建立肺鳞癌PDX 表征；从同一患者样本中建立PDX、器官和球体；提供多个 转基因模型、PDX和三维培养方法；建立KPC转基因模型、生物信息库 胰腺模型和组织，以及患者来源的模型(异种移植、器官和球体)。

As a result of CCSG strategic planning and evaluation of services offered, the former Mouse Tumor Models shared resource (MTM) was renamed the Experimental Tumor Model Shared Resource (ETM). ETM has expanded its experimental models to include xenografts, organoid and spheroid models from patient derived samples. The major objective is to provide high quality, cost-effective, custom services tailored to the needs of individual investigators and to provide a consolidated resource of experimental models and technical expertise for all aspects of in vivo experiments to support the research efforts of CCSG investigators. The ETM shared resource served a total of 65 Roswell users, of which 60 (92%) were CCSG members. ETM is a great value for their users as there are no commercial options that offer the full range of services for in vivo studies that are provided by ETM. The consolidated breeding colonies for the production of animals offer a profound savings over buying the transgenic mice through commercial sources. The ETM resource has two primary areas of focus: (i) experimental studies that utilize cancer models which includes maintaining models; biobank of tissues from each model; and providing technical support for treatment, evaluation of response and tissue procurement; and (ii) histological services for murine tissues. ETM service includes the full spectrum starting with study protocol development, providing experimental models, performing in vivo treatments to tissue procurement and histological staining with standardize murine IHC protocols. The histology services offered by ETM are focused on murine tissues. The resource maintains breeding colonies of several widely used models of prostate cancer. The ETM also maintains transgenic-based models of sarcoma, lung, pancreas (in development), and a carcinogen-induced model of bladder cancer. The ETM establishes and maintains a variety of patient-derived xenograft (PDX) models. The Specific Aims of the ETM are: 1) To provide a consolidated resource for conducting in vivo studies utilizing experimental models of cancer including biospecimens, experimental models and technical support for in vivo studies, and 2) To provide tissue procurement and histological support for research involving experimental models. ETM plans to establish new PDX models from clinical samples; establish lung squamous carcinomas PDXs with deep molecular characterization; establish PDXs, organoids and spheroids from the same patient samples; provide multiple transgenic models, PDX and 3-D culture methods; and establish the KPC transgenic model, biorepository of pancreatic models and tissues, and patient derived models (xenograft, organoids and spheroids).