Mechanistic studies of a bifurcating flavoprotein
分叉黄素蛋白的机理研究
基本信息
- 批准号:10640091
- 负责人:
- 金额:$ 29.62万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-07-01 至 2024-06-30
- 项目状态:已结题
- 来源:
- 关键词:BiologicalCarbon DioxideCellsCoenzyme AComplexCoupledCryoelectron MicroscopyDefectDependenceDiseaseElectron TransportElectron transfer flavoproteinElectronsEnsureEnzymesEventFamilyFerredoxinFerredoxin-NAD+ reductaseFlavodoxinFlavoproteinsFunctional disorderGoalsHumanIndividualIron-Sulfur ProteinsIsotopesKineticsMetabolismMitochondriaMolecularMonitorMotionMultienzyme ComplexesNADHNADPOxidantsOxidation-ReductionOxidoreductasePathway interactionsPhysiologicalPlayProcessPyrobaculumPyruvateQuinonesReactionRoleSolventsSpectrophotometryStructureSystemThermodynamicsUbiquinoneVitamin K 2Workdeprotonationelectron donorelectron-transferring-flavoprotein dehydrogenaseenzyme modelexperimental studyfatty acid oxidationhuman diseasemembermicroorganismprotonationsample fixation
项目摘要
Project Summary
Electron-transferring flavoproteins (ETFs) are a large and broadly distributed family of
electron carriers that play critical roles in a variety of metabolic processes, including b-oxidation of
fatty acids in humans. Defects in ETFs give rise to several specific disease states. A subset of the
ETF family constitute a recently discovered class of complex flavoproteins that catalyze the coupled
reduction of high- and low-potential electron acceptors using a median-potential electron donor in a
process referred to as electron bifurcation. An example of such a bifurcating ETF is the EtfABCX
system from Pyrobaculum aerophilum. This enzyme accepts reducing equivalents from NADH,
passing the reducing equivalents on to the high-potential menaquinone and the low-potential iron-
sulfur protein ferredoxin in a manner that is overall thermodynamically favorable. The proposed
work will examine in detail the kinetics of electron bifurcation as catalyzed by EtfABCX, critically
evaluating the involvement of protonation/deprotonation processes associated with the primary
electron transfer events.
项目摘要
电子转移黄素蛋白(ETFs)是一个大的和广泛分布的家族,
电子载体,在各种代谢过程中发挥关键作用,包括β-氧化,
人体内的脂肪酸ETF的缺陷会导致几种特定的疾病状态。的子集
ETF家族构成了最近发现的一类复杂的黄素蛋白,其催化偶联的
使用中位电子供体还原高电位和低电位电子受体,
这个过程称为电子分叉。这种分叉ETF的一个例子是EtfABCX
Pyrobaculum aerophilum.这种酶接受来自NADH的还原当量,
将还原当量传递到高电位甲萘醌和低电位铁上-
硫蛋白铁氧还蛋白以总体上有利的方式。拟议
工作将详细研究EtfABCX催化的电子分叉动力学,
评估与初级质子化/去质子化过程相关的参与
电子转移事件
项目成果
期刊论文数量(7)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
A new era for electron bifurcation.
- DOI:10.1016/j.cbpa.2018.07.026
- 发表时间:2018-12
- 期刊:
- 影响因子:7.8
- 作者:
- 通讯作者:
Correlating Conformational Equilibria with Catalysis in the Electron Bifurcating EtfABCX of Thermotoga maritima.
- DOI:10.1021/acs.biochem.3c00472
- 发表时间:2024-01-02
- 期刊:
- 影响因子:2.9
- 作者:Murray, Daniel T.;Ge, Xiaoxuan;Schut, Gerrit J.;Rosenberg, Daniel J.;Hammel, Michal;Bierma, Jan C.;Hille, Russ;Adams, Michael W. W.;Hura, Greg L.
- 通讯作者:Hura, Greg L.
Spectral deconvolution of redox species in the crotonyl-CoA-dependent NADH:ferredoxin oxidoreductase from Megasphaera elsdenii. A flavin-dependent bifurcating enzyme.
- DOI:10.1016/j.abb.2021.108793
- 发表时间:2021-04-15
- 期刊:
- 影响因子:3.9
- 作者:Vigil W Jr;Niks D;Franz-Badur S;Chowdhury N;Buckel W;Hille R
- 通讯作者:Hille R
Characterization of the Membrane-Associated Electron-Bifurcating Flavoenzyme EtfABCX from the Hyperthermophilic Bacterium Thermotoga maritima.
- DOI:10.1021/acs.biochem.3c00473
- 发表时间:2023-12-19
- 期刊:
- 影响因子:2.9
- 作者:Ge, Xiaoxuan;Schut, Gerrit J.;Tran, Jessica;Poole II, Farris L.;Niks, Dimitri;Menjivar, Kevin;Hille, Russ;Adams, Michael W. W.
- 通讯作者:Adams, Michael W. W.
Xanthine Oxidase-A Personal History.
- DOI:10.3390/molecules28041921
- 发表时间:2023-02-17
- 期刊:
- 影响因子:4.6
- 作者:Hille, Russ
- 通讯作者:Hille, Russ
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Russ Hille其他文献
Russ Hille的其他文献
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{{ truncateString('Russ Hille', 18)}}的其他基金
Mechanistic studies of a bifurcating flavoprotein
分叉黄素蛋白的机理研究
- 批准号:
10410411 - 财政年份:2020
- 资助金额:
$ 29.62万 - 项目类别:
Mechanistic studies of a bifurcating flavoprotein
分叉黄素蛋白的机理研究
- 批准号:
10387414 - 财政年份:2020
- 资助金额:
$ 29.62万 - 项目类别:
Mechanistic studies of a bifurcating flavoprotein
分叉黄素蛋白的机理研究
- 批准号:
10201670 - 财政年份:2020
- 资助金额:
$ 29.62万 - 项目类别:
Structure/activity studies of two molybdenum enzymes
两种钼酶的结构/活性研究
- 批准号:
7892110 - 财政年份:2009
- 资助金额:
$ 29.62万 - 项目类别:
Structure/activity studies of two molybdenum enzymes
两种钼酶的结构/活性研究
- 批准号:
7441263 - 财政年份:2005
- 资助金额:
$ 29.62万 - 项目类别:
Studies of environmentally relevant molybdenum enzymes
环境相关钼酶的研究
- 批准号:
7117987 - 财政年份:2005
- 资助金额:
$ 29.62万 - 项目类别:
Structure/activity studies of two molybdenum enzymes
两种钼酶的结构/活性研究
- 批准号:
6961309 - 财政年份:2005
- 资助金额:
$ 29.62万 - 项目类别:
Structure/activity studies of two molybdenum enzymes
两种钼酶的结构/活性研究
- 批准号:
7437368 - 财政年份:2005
- 资助金额:
$ 29.62万 - 项目类别:
Studies of environmentally relevant molybdenum enzymes
环境相关钼酶的研究
- 批准号:
7683053 - 财政年份:2005
- 资助金额:
$ 29.62万 - 项目类别:
Structure/activity studies of two molybdenum enzymes
两种钼酶的结构/活性研究
- 批准号:
7094249 - 财政年份:2005
- 资助金额:
$ 29.62万 - 项目类别:
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