Core C: Myeloid cell and atherosclerosis core

核心C：骨髓细胞和动脉粥样硬化核心

• 批准号: 10642743
• 负责人: Jenny E. Kanter
• 金额: $ 18.87万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-15 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10642743
• 关键词: Aorta; Arterial Fatty Streak; Arteries; Atherosclerosis; Bioinformatics; Biological Assay; Biology; Cardiovascular Diseases; Cell Compartmentation; Cells; Characteristics; Complement; Confocal Microscopy; Data; Data Set; Diabetes Mellitus; Digestion; Ensure; Family; Flow Cytometry; Gene Expression; Gene Expression Profiling; Histologic; Histology; Immunohistochemistry; Individual; Laboratories; Lesion; Lesion by Morphology; Lipoproteins; Macrophage; Measurement; Molecular; Morphology; Myeloid Cells; Procedures; Process; Program Research Project Grants; Protocols documentation; RNA analysis; Regulation; Reproducibility; Research; Research Personnel; Services; Site; Stains; Standardization; Techniques; Technology; Tissues; Triglycerides; Valsalva sinus; analytical method; analytical tool; cell type; cost effectiveness; histological stains; immunocytochemistry; instrumentation; laser capture microdissection; mouse model; novel; programs; single-cell RNA sequencing; tissue preparation; transcriptome sequencing

The Myeloid Cell and Atherosclerosis Core (Core C) within the Program Project “Triglycerides, Diabetes and Cardiovascular Disease” will provide individual projects with analytical techniques for thorough analysis of lesions of atherosclerosis from the different mouse models used within the program. These analyses will include histological staining methods, lesion size measurements, lesion morphology characterization, immunohistochemistry, and laser capture microdissection for analysis of gene expression. The core will also provide expertise and bioinformatics analysis of RNA-sequencing and single cell RNA-sequencing data as a complement to the Program Investigators’ projects. This Core’s repertoire of high-quality analyses of myeloid cells, atherosclerosis and other tissues studied within the Program Project will substantially enhance the scientific progress of the four individual projects.

“甘油三酯、糖尿病和动脉粥样硬化"项目中的髓样细胞和动脉粥样硬化核心（核心C） 心血管疾病”将为个别项目提供分析技术， 动脉粥样硬化的病变，从不同的小鼠模型中使用的程序。这些分析将 包括组织学染色方法、损伤尺寸测量、损伤形态表征 免疫组织化学和激光捕获显微切割用于分析基因表达。核心还将 提供RNA测序和单细胞RNA测序数据的专业知识和生物信息学分析， 补充项目调查员的项目。该核心的高质量骨髓分析库 细胞，动脉粥样硬化和其他组织的研究计划项目将大大提高 四个项目的科学进展。